Navigation Links
A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

solution wellplate reader. In combination with the capability of imaging large area specimens, automated image acquisition and analysis, the wide field-of-view system can be equipped with multiple slide holders to realize a higher degree of imaging automation. In addition, more efficient methods of image archiving and large image handling and browsing will be developed.

The wide field-of-view confocal imaging system presented in this paper has three excitation lasers and six detection channels, three for fluorescence and reflected-light imaging, and three for bright field. In applications where multiple fluorescence probes and a wider variety of dyes are used, separation of the spectra on the basis of emission wavelengths may not be specific enough, and excitation wavelength must also be selected. Therefore, methods such as the use of an acousto-optic tunable filter (AOTF) for the selection of laser lines may need to be adopted.64 AOTF simultaneously selects the wavelength and controls the degree of attenuation applied.64 When using an AOTF as a tunable dichroic beamsplitter, multiple specific wavelengths can be deflected towards the objectives simultaneously or in sequence. A polarizing beamsplitter can also be used in place of the pri- mary dichroic beamsplitter, which preferentially reflects polarized light (the laser light), while transmitting the nonpolarized fluorescence light to the detection optics.64 When using dyes with overlapping spectra, selection of excitation wavelengths rather than emission wavelengths must also be made. In most cases this can be adjusted sufficiently rapidly to allow "line by line" sequential scanning, where each line is scanned twice - once using one excitation wavelength and one detector, the next using another excitation wavelength and detector.64

Fluorescence detection with dichroic mirrors and barrier filters can be highly specif
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals
2. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
3. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
4. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
5. Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system
6. Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system
7. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
8. Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system
9. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
10. A technique for increasing yields in bioreactors and disposable cell-culture systems
11. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
Post Your Comments:
(Date:12/24/2014)... 23, 2014 Pipette.com announces their ... followers can submit their #HolidayInTheLab pictures for a chance ... mates. , For those struggling to think of a ... Holiday in the Lab Contest provides the ideal holiday ... to send pictures of their chemistree, holiday sweaters, ...
(Date:12/24/2014)... Dec. 23, 2014 Sigma-Aldrich Corporation (NASDAQ: ... waiting period under the Hart-Scott-Rodino Antitrust Improvements Act ... on December 22, 2014, thereby completing the U.S. ... the acquisition of the Company by Merck KGaA, ... satisfies another condition to closing the transaction, which ...
(Date:12/22/2014)... , Dec. 22, 2014   Synthetic ... developer of pathogen-specific therapies for serious infections ... the microbiome, today announced positive topline safety ... clinical trial of SYN-004, the Company,s investigational ... Clostridium difficile (C. difficile) infection, ...
(Date:12/22/2014)... , Dec. 22, 2014  ( www.competitivehealth.com ) ... an online medical bill review and advocacy service, has ... the FREE WellCard Savings discount health services marketplace. ... that was more than they expected to pay. As ... health care costs, WellCard Savings is pleased to offer ...
Breaking Biology Technology:Major Pipette Distributor Pipette.com Announces their Holiday in the Lab Contest 2Acquisition of Sigma-Aldrich by Merck KGaA, Darmstadt, Germany, Receives U.S. Antitrust Clearance 2Acquisition of Sigma-Aldrich by Merck KGaA, Darmstadt, Germany, Receives U.S. Antitrust Clearance 3Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 2Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 3Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 4Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 5WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2
... and CEO of San Diego-based Bio-Matrix Scientific Group Inc. (OTC ... cell cord blood banking is a "huge business opportunity for ... the world are now eagerly starting their own stem cell ... are naturally interested in providing cord blood banking facilities to ...
... /PRNewswire-Asia-FirstCall/ -- China-Biotics, Inc.,(Nasdaq: CHBT ) ("China-Biotics," ... development, manufacture, marketing and,distribution of probiotics dietary supplements, today ... EDT on Wednesday, July 15, 2009, to discuss the,Company,s ... ended March,31, 2009. , To ...
... 9 Schering-Plough Corporation (NYSE: SGP ... country operation in Japan, has received marketing approval ... treatment of major depressive disorder.(1) The product was ... Schering-Plough and Meiji Seika will market the product ...
Cached Biology Technology:Stem Cell Cord Blood 'Banking' Is Huge Global Business for Hospitals, Says CEO of Bio-Matrix Scientific Group Inc. 2China-Biotics, Inc. Announces Conference Call to Discuss Fourth Quarter and Full Year 2009 Financial Results 2REMERON(R) Now Approved in Japan for the Treatment of Depression in Adult Patients 2REMERON(R) Now Approved in Japan for the Treatment of Depression in Adult Patients 3REMERON(R) Now Approved in Japan for the Treatment of Depression in Adult Patients 4REMERON(R) Now Approved in Japan for the Treatment of Depression in Adult Patients 5
(Date:12/3/2014)... IRVINE, Calif. , Dec. 2, 2014 /PRNewswire/ ... of globally deployed, innovative test solutions for military, ... the newest version of its successful TS-900 ... the performance and features of high-end systems to ... with outstanding value compared to traditional ATE. ...
(Date:11/21/2014)... Nov. 20, 2014 Strict laws against distracted ... piloting the North American and European automotive sector towards ... gesture recognition systems that are intuitive and able to ... the industry. New analysis from Frost ... Market in Europe and ...
(Date:11/18/2014)... 2014   News Highlights: ... develop The Partners Data Lake, an agile data ... Lake will allow researcher and clinicians to explore ... the lives of patients , The Partners ... across the Partners system, breaking down physical barriers ...
Breaking Biology News(10 mins):Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 2Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 3Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 5
... Yale School of Medicine have developed a simple urine ... but serious hypertensive complication of pregnancy. Dubbed the ... test accurately predicted preeclampsia in a study of 347 ... preventive care to pregnant women. The research will be ...
... , BLUE BELL, Pa. , Feb. 3 ... its Mexican subsidiary has been awarded a contract by the ... Affairs/National Citizen Registry) to create and manage an advanced citizen ... database with iris, fingerprint and facial biometric data on up ...
... the Himalayan mountains are thinning is not disputed. However, ... the causes. Lawrence Berkeley National Laboratory scientist Surabi Menon ... commonly blamed culpritgreenhouse gases, such as carbon dioxidefrom other ... melting. Menon and her collaborators found that airborne black ...
Cached Biology News:Yale-developed test can help predict and diagnose preeclampsia 2Unisys Awarded Contract to Provide Advanced Biometric Citizen Identification Solution to Government of Mexico 2Unisys Awarded Contract to Provide Advanced Biometric Citizen Identification Solution to Government of Mexico 3Black carbon a significant factor in melting of Himalayan glaciers 2Black carbon a significant factor in melting of Himalayan glaciers 3
... Molecular Imaging's proprietary Focus™ detector technology, while ... the R4. The microPET Focus 120 ... avaiable PET scanner. Focus scanners also ... scinitallor based PETsystems. The combination of ...
... The Freedom EVO series offers versatile ... to successfully automate a wide variety of ... in four different base sizes (75, 100, ... personnel access to a platform that will ...
... This product has been developed to significantly ... labeled proteins. Stability is increased at ... 2-8 centigrade. This product is subjected ... mercury or azide. This formulation insures ...
... The StatMatic I provides a convenient and ... culture work. Used with an eight-port disposable ... not possible with multi-channel pipettes. The eight-port ... in volumes of 100, 200, 250, or ...
Biology Products: