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A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

ide. Figure 15 is a fluorescence image of a 20 by 30 mm area of the whole mouse section, displaying the thoraco-abdominal region of the mouse embryo from Fig. 14. In Fig. 16, details of cardiac region are observed. The image has been rotated to the left with respect to Fig. 14 and Fig. 15. Heart valves can be seen in the middle of the section, which shows mainly the atrial region of the heart. A chain of thoracic vertebra sections can be seen on the left. Figure 17 is an actual pixel image of a tiny part of Fig. 14 at 2.0/1.0 μm optical/pixel resolution.

4. CONCLUSIONS AND FUTURE DIRECTIONS

The new wide field-of-view confocal microscopy imaging system will have wide application in pathology and drug discovery, because of its capability of imaging large tissue samples and entire slides of tissue and DNA microarrays with microscopic resolution in a single scan, in both confocal fluorescence and reflectance imaging, and in RGB brightfield. The proprietary laser scan lenses, auxiliary optics, electronics, and computer and software for the confocal imaging system make the image acquisition more efficient than other methods. By using the new microscopy system, it is possible to perform high throughput screening of drug effects, e.g., using slides of whole zebrafish embryos. Many researchers have started to use zebrafish embryo models for large scale whole organism screening.63 By using this new confocal imaging system, for example, zebrafish embryos held in microwell plates that have been subjected to various treatments across the wells could be mounted on a single microscope slide and evaluated together in a single scan, to pinpoint differences in gene expressions through fluorescence in situ hybridization, or morphology through routine H & E staining. With a little modification, the wide field-of-view confocal microscopy imaging system can be turned into a high re
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