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A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

and drug effects in humans, is increasingly being used for testing in drug discovery. Zebrafish have all of the equivalent organs found in mammals, and most human genes have homologues in zebrafish.60-62

Figures 10 - 13 are inverted fluorescence images of coronal sections of zebrafish larvae stained with H & E, plastic sectioned at 1 μm thickness. Figure 10 is an overview image of the slide taken using a green (535 nm) laser and a 700/75 nm bandpass emission filter. There are six larva sections in it. Figure 11 is an overview of the inverted fluorescence image of one of the zebrafish larva sections in Fig. 10, depicting entire organism in a coronal section. It is recorded with green laser (532 nm) excitation and a 570/50 nm bandpass emission filter, at 0.5/0.25 μm optical/pixel resolution. Figure 12 is a transposed and magnified part of the fluorescence image in Fig. 11. This section view depicts lower coronal section of the transition region from pharyngeal portion to abdomen of the zebrafish larva. Figure13 is the actual pixel image of an eye of the zebrafish larva shown in Fig. 11. The overlaying cornea, round lens and retinal layers are observed clearly.

3.3. Whole Mouse Section Imaging

Figures 14 - 17 are inverted fluorescence images of or a part of a sagittal whole mouse section stained with Thioflavin-S. The images were recorded using green (535 nm) laser excitation and 700/75 nm bandpass emission filter. Figure 14 is an overview of a 20 by 65 mm whole mouse section on a standard microscope slide (25.4 by 76.2 mm) at 2.0/1.0 μm optical/pixel resolution, showing the entire saggital section with the anterior (head) end on the right and the (posterior) tail end on the left s
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