( 59 and fluorescence microscopy is cruci...)A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

Figures 2 - 4 show RGB brightfield images of proliferating cells in a tumor tissue section 10.5 by 10 mm in size, immunostained for the proliferation marker MIB-1 (Ki67). These images were taken at a pixel resolution of 1 μm, using the laser scan lens with an optical resolution of 2 μm. Fig. 3 is a magnified view of part of Fig. 2, in which the round dark spots are nuclei of cancerous cells immunostained with the MIB-1 marker. Figure 4 is a screen capture of part of the image at the actual pixel size with 1 μm pixel resolution. Cancerous cells immunostained with the proliferation marker MIB-1 (Ki67) are clearly shown.

Figures 5 - 7 display fluorescence images of a tissue microarray, recorded with a blue (488 nm) laser excitation and a 570/50 nm emission filter at 2 μm optical (1 μm pixel) resolution. The fluorescence images are intentionally inverted in greyscale, which makes bright fluorescence object appear dark, for ease of printing in black and white. The slide label part of the image is not shown. Figure 6 is a magnified image of a part of Fig. 5, with nine tissue core sections in it. Figure 7 is the actual pixel image of one of the tissue core sections in Fig. 5 and Fig. 6.

Figure 8 shows the fluorescence image of a DNA microarray slide, with the excitation lasers being green (535 nm) for Cy3 fluorophore and red (633 nm) for Cy5. The spots are 150 μm in diameter. Figure 9 is a fluorescence image of a Cy3 DNA calibration biochip with features of 15 μm squares, detected with green (532 nm) laser excitation and a 570/50 nm emission filter.

3.2. Imaging Zebrafish Slide

Zebrafish, as a vertebrate model organism for studying gene function
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