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A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

VERY AND PATHOLOGY

The imaging applications of the new wide field-of-view confocal imaging system in drug discovery and pathology are described in this section. Selected imaging specimens include a large tissue section, a tissue microarray, a slide of zebrafish larva sections, a whole mouse embryo slide, a DNA microarray slide. Most of the images are presented in three formats, an overview of the whole slide or a major part of a whole slide, a magnified portion of the specimen, and a portion of the actual pixel (original size) image of the specimen. The overview images show the large field of view of the wide field-of-view imaging system; the zoomed in images display some interesting structures in the specimens; and the actual pixel images indicate the resolution limit of the microscopy imaging system. The images shown in this section are all computer screen captures of 1024 by 768 resolution. The pixel resolution is twice that of the optical resolution based on the Nyquist Theorem. For example, a pixel resolution of 1 μm measured at the specimen is required to correctly capture and display an image from a microscope whose optical resolution is 2 μm. Most of the images presented in this Section are taken at 2.0/1.0 μm optical/pixel resolution, except for the images shown in Figure 11 to 13, which are taken at 0.5/0.25 μm optical/pixel resolution.

3.1. Tissue, Tissue Microarray and DNA Microarray Imaging

In pathology and drug discovery, confocal microscopy and conventional widefield light microscopy will continue to play an important role, for both morphological investigations and molecular biological diagnostics. Morphological diagnosis, as the gold standard in soft tissue diagnostic pathology, is facing a challenge from molecular diagnostics which is providing the basis for reclassification of some well-entrenched morphological entities,
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