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A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

tential provided by TMA technology in drug discovery research by offering the capability of imaging the whole slide efficiently with a single scan and using the same data acquisition condition, so that TMA users will not have to look at the array spots one by one using other imaging techniques.37, 38

This new confocal microscope technology enabling confocal fluorescence and brightfield imaging of large biomedical specimens is described in the next section. Applications of the wide field-of-view confocal imaging system in pathology and drug discovery research, for example, imaging large tissue specimens, tissue microarrays, and zebrafish sections, are also reported in this paper.

2.WIDE FIELD-OF-VIEW CONFOCAL LASER SCANNING SYSTEM

The wide field-of-view confocal imaging system consists of an optics module, an electronics module and a com- puter and software. The optics module is the main part of the system, through which the unique wide field-of-view imaging function of the system can be realized. The electronics module provides system control, signal process- ing and data acquisition. The computer and software coordinate the imaging system through the electronics, providing such functions as user interface, and image display and data archiving.

The optical schematic of the system is shown in Fig. 1. The raster scan of the focused laser spot across the specimen is realized by beam scanning in the X (lateral) direction and stage scanning in the Y (longitudinal) direction of the specimen, to produce images in the standard tagged image file format (TIFF). The main com- ponents of the optical module are the laser scan lens, excitation/illumination optics, and fluorescence and RGB (red, green and blue) detection optics. The system is capable of
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