Navigation Links
A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

ectives must acquire a large number of small images of the specimen, and then tile them together, which is tedious, inefficient and error-prone.

Microscopic imaging of large specimens is of considerable interest to life science, partly because of the high throughput efficiency it offers. However, there are only a few academic investigations of wide field-of-view microscopes reported elsewhere, e.g., microphotography with a thin white light slice,45 using single spatial frequency grid pattern,46 and objective lens array based large field-of-view imaging.47, 48 The sizes of specimen that can be imaged with conventional widefield microscopes are limited by the field-of-view of the microscope objectives. With the increase of power (magnification) and resolution (numerical aperture), the field-of-view becomes smaller and smaller. When using standard microscope objectives for scanning laser imaging, except for stage-stage scanning, in both the beam-beam and beam-stage scan methods, the scan size is limited by the field-of-view of the objective lenses. In many clinical studies, it is desirable to acquire images of whole diseased tissue sections, such as those of tumors, while retaining microscopic resolution.49

The wide field-of-view confocal microscopy imaging system reported here can be used for the examination of morphology and molecular biology images of clinical tissue specimens, and high throughput screening in drug discovery through efficient imaging of microarray slides. It is capable of operating in brightfield, reflection and epi- fluorescence imaging modes. A red, green and blue laser is used to produce fluorescence, brightfield and reflection images. This new confocal system makes fluorescence examination of large specimens with microscopic resolution possible for the first time without tiling. The wide field-of-view will help fully realize the high throughput po
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals
2. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
3. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
4. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
5. Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system
6. Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system
7. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
8. Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system
9. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
10. A technique for increasing yields in bioreactors and disposable cell-culture systems
11. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
Post Your Comments:
(Date:12/19/2014)... iLab Solutions, the global leader in core facility ... the new Director of Institutional Implementations and Sarah Becker ... new leadership positions were created to support iLab’s mission ... continues to meet the needs of its growing customer ... been deployed at over 450 core facilities across more ...
(Date:12/19/2014)... CA (PRWEB) December 18, 2014 With ... limited time special on Sartorius Biohit products . ... on mLINE pipettes, Picus Electronic Pipette Trade-in Program, and ... Health and Safety officers around the world. They are ... pipetting. Other features include:, ,     Full volume ...
(Date:12/19/2014)... , Dec. 18, 2014   ISN ... & Safety Performance Indicators publication series. With ... of activity reported in 2013 within its online contractor ... and safety key performance indicator (KPI) statistics. ... Clients, U.S. publication is available in two ...
(Date:12/17/2014)... Based on the revenues gained from surgical ... dominated by four major players contributing to approximately ... include GE Healthcare (U.K.), Siemens AG (Germany), Philips ... Report Copy at http://www.marketsandmarkets.com/Market-Reports/surgical-imaging-market-210534462.html , The ... to its strong product portfolio, which includes its ...
Breaking Biology Technology:iLab Solutions Announces Two New Leadership Positions to Meet the Needs of Its Growing Customer Base 2Major Pipette Distributor Pipette.com Announces Sartorius Year End Specials 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 4
... ASSAYS, , , , , ... DATA , , HIGHER THROUGHPUT ... , Every major pharmaceutical company uses the,Molecular Devices FLIPR ... Calcium Assay Kit from Molecular,Devices provides the easiest way to ...
... Su, M.S., ... , , INTRODUCTION , ... Of thespectrophotometric methods available, UV absorbance measurement at 260 nm ... microplateformat. 1 However, A 260 measurements do not ...
... INTRODUCTION ... of a dissolution apparatus is necessary to produce accurate results ... comparison between batches and between laboratories. USP requirements for calibration ... Dissolution. 1 Each individual apparatus (i.e. spindle ...
Cached Biology Technology:FLIPR Calcium Assay Kit Datasheet 2FLIPR Calcium Assay Kit Datasheet 3DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37) 2DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37) 3DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37) 4Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 2Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 3Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 4Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 5Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 6Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 7Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 8Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 9
(Date:12/17/2014)... 2014  HITLAB SM announced today its ... to confirm its adherence to current U.S. Food ... HITLAB to conduct regulated smart device and smart ... patient safety and research quality. "HITLAB ... and delivery with innovative technology," said Laura ...
(Date:12/15/2014)... --Research and Markets ( http://www.researchandmarkets.com/research/87pph8/global_facial ) ... Facial Recognition Market 2015-2019" report to their ... Facial recognition is a technology used ... measures the overall facial feature of a person ... distance between eyes. Facial recognition is used for ...
(Date:12/11/2014)... 10, 2014  Data Sciences International (DSI), the ... a new series of digital telemetry implants to ... M series, part of the PhysioTel™ Digital platform, ... possible physiologic data when incorporating functional endpoints into ... to toxicology studies has evolved from short ECG ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3New telemetry implants expected to change how large animal toxicology studies are conducted 2
... -- Yoho National Park,s 505-million-year-old Burgess Shale home ... very primitive human relative is one of the ... century after its discovery, a compelling sequel has been ... new Burgess Shale fossil site has been located that ...
... cancer, also kills a fungus that causes a deadly brain ... to new treatments for a disease that kills more HIV/AIDS ... open-access journal of the American Society for Microbiology (ASM.) ... see if tamoxifen can be used as a drug in ...
... The Translational Genomics Research Institute (TGen) has uncovered possible ... according to a first-of-its-kind study published in the scientific journal ... uncovered by TGen now can be used to identify potential ... estimated 45,000 patients in the U.S. each year whose cancer ...
Cached Biology News:Researchers discover 'epic' new Burgess Shale site in Canada's Kootenay National Park 2Breast cancer drug fights fungal disease 2TGen study uncovers possible genetic markers in breast cancer that spreads to the brain 2
... Immunogen: Synthetic Peptide: M(1) E F ... G P P T M D L E(19) ... mouse cells.,PA1-072 has been successfully used in Western ... detects an ~86 kDa protein representing Mint3 from ...
... 3.5 is a user-friendly statistical software package ... to perform statistical analysis without being a ... your data and research and SigmaStat suggests ... against the tests underlying assumptions and suggests ...
... a chemiluminescent signal enhancing reagent that ... the signal obtained with all ECL ... Plus is derived from a powerful, ... or chemiluminescent substrate enhancers, ChemiLucent™ Plus ...
... Control at its Best: DASGIPs Process ... Online Changes & comprehensive documentation for ... parameters auch as pH, dissolved oxygen, ... and changeable online. Profiles, e.g. constant ...
Biology Products: