Navigation Links
A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

ectives must acquire a large number of small images of the specimen, and then tile them together, which is tedious, inefficient and error-prone.

Microscopic imaging of large specimens is of considerable interest to life science, partly because of the high throughput efficiency it offers. However, there are only a few academic investigations of wide field-of-view microscopes reported elsewhere, e.g., microphotography with a thin white light slice,45 using single spatial frequency grid pattern,46 and objective lens array based large field-of-view imaging.47, 48 The sizes of specimen that can be imaged with conventional widefield microscopes are limited by the field-of-view of the microscope objectives. With the increase of power (magnification) and resolution (numerical aperture), the field-of-view becomes smaller and smaller. When using standard microscope objectives for scanning laser imaging, except for stage-stage scanning, in both the beam-beam and beam-stage scan methods, the scan size is limited by the field-of-view of the objective lenses. In many clinical studies, it is desirable to acquire images of whole diseased tissue sections, such as those of tumors, while retaining microscopic resolution.49

The wide field-of-view confocal microscopy imaging system reported here can be used for the examination of morphology and molecular biology images of clinical tissue specimens, and high throughput screening in drug discovery through efficient imaging of microarray slides. It is capable of operating in brightfield, reflection and epi- fluorescence imaging modes. A red, green and blue laser is used to produce fluorescence, brightfield and reflection images. This new confocal system makes fluorescence examination of large specimens with microscopic resolution possible for the first time without tiling. The wide field-of-view will help fully realize the high throughput po
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals
2. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
3. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
4. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
5. Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system
6. Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system
7. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
8. Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system
9. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
10. A technique for increasing yields in bioreactors and disposable cell-culture systems
11. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
Post Your Comments:
(Date:8/27/2014)... Guggenheim Securities, the investment banking and capital ... of veteran equity analyst Charles "Tony" Butler as a ... focus on the biotech/biopharma sector. " Tony ... and a broad network of relationships in his industry," ... Head of Equities at Guggenheim Securities. "His insights will ...
(Date:8/27/2014)... Research and Markets has announced ... & Paper Industry Report 2014" report to their ... & Paper Industry Report 2014 is a professional and ... enzyme for pulp & paper industry. The ... definitions, classifications, applications and industry chain structure. The enzyme ...
(Date:8/27/2014)... 27, 2014 Tris Pharma, a specialty pharmaceutical ... announced that it has been selected as an award ... 2014 Awards for Excellence competition in the ... panel of judges from SCORE (Counselors to America,s Small ... be honored at a gala event to be held ...
(Date:8/27/2014)... After the boom of the energy drink ... craze is going to be the stress relief drinks. ... Stress Relief energy pattern which can be applied to ... beverage, including minerals, herbs and liquids. Stress Relief generates ... into rapid recovery and helping to adapt more quickly. ...
Breaking Biology Technology:Guggenheim Securities Hires Analyst Charles "Tony" Butler to Expand Healthcare Coverage 2Global Enzyme for Pulp & Paper Industry Report 2014 2Tris Pharma Selected as an award winner in the New Jersey Business & Industry Association's 2014 Awards for Excellence 2Stress Relief: 1 Way to De-Stress Your Beverage Formula 2Stress Relief: 1 Way to De-Stress Your Beverage Formula 3Stress Relief: 1 Way to De-Stress Your Beverage Formula 4
... Data Generated From Both mRNA ... , Clustering analysis is ... a visual representation of complex data and a method for measuring ... for clustering , microarray data are: Hierarchical, K-means and Self-organizing ...
... now offers a complete Gene Expression Profiling Service* for Affymetrix , ... Full Service offering, , no project is ... consultation with Ambion's team of experienced array and ... Ambion's long history of providing innovative, , ...
... Isolate total RNA and protein from cultured cells or tissues ... nuclear and cytoplasmic RNA and protein from cultured , cells ... , Ambion's new PARIS Kit uses a fast, ... both RNA and native protein , from the same experimental ...
Cached Biology Technology:Analysis of Microarray Data 2Analysis of Microarray Data 3Analysis of Microarray Data 4Analysis of Microarray Data 5Analysis of Microarray Data 6Analysis of Microarray Data 7Ambion Offers Gene Expression Profiling Service 2Ambion Offers Gene Expression Profiling Service 3A Better Way to Isolate RNA AND Protein From the Same Sample 2A Better Way to Isolate RNA AND Protein From the Same Sample 3A Better Way to Isolate RNA AND Protein From the Same Sample 4A Better Way to Isolate RNA AND Protein From the Same Sample 5
(Date:8/27/2014)... Thousands of fishing traps are lost or abandoned each ... as derelict traps, which continue to catch fish, crabs, ... in losses to habitat, fisheries, and the watermen who ... to a newly published NOAA study. , The report, ... the first of its kind to examine the derelict ...
(Date:8/27/2014)... Happy Camp Complex of fires had consumed 24,939 acres ... consumed 35,530 as of eight hours ago. , ... Ranger District of the Klamath National Forest when a ... All but three of those fires are now 100 ... fires and their size at containment: Delta, 150 acres; ...
(Date:8/26/2014)... over a colony of harmful bacteria, biofilms make ... protected in a biofilm pose a significant health ... to treatment, and biofilm-protected bacteria account for some ... and are 50 to 1,000 times more resistant ... essence, we may have stumbled onto a magic ...
Breaking Biology News(10 mins):NOAA's Marine Debris Program reports on the national issue of derelict fishing traps 2Happy Camp and July Fire Complexes in California 2Breakthrough antibacterial approach could resolve serious skin infections 2Breakthrough antibacterial approach could resolve serious skin infections 3
... According to a new report on regional marine research ... research about fisheries, ocean health, coastal hazards and climate ... protect the region,s annual $32-billion ocean-related economy, according to ... Grant programs. The report, West Coast Regional Marine ...
... German . , Several thousand test fields are ... They permit the rapid analysis of substances, e.g. for diagnosing ... use for DNA testing. When it comes to proteins, such ... have a defined three-dimensional structure by which they can interact ...
... Davis, CA Researchers from the University of California, Davis ... of the fragile X mental retardation 1 (FMR1) protein (FMRP), ... report by Iwahashi et al, "A quantitative ELISA assay for ... July 2009 issue of the Journal of Molecular Diagnostics ...
Cached Biology News:West Coast needs more research on fisheries, marine science, climate change 2Proteins in gel 2Measuring intellectual disability 2
Multidrop Micro high speed 1 l bulk reagent dispenser rapidly, precisely and accurately dispenses microvolumes of 1-50 l into 384- and 96-well microplates....
...
...
Buckets for tube holder inserts...
Biology Products: