Navigation Links
A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

Gang Lia, Savvas Damaskinosa, Arthur E. Dixona and Lucy E. J. Leeb

a Biomedical Photometrics Inc., A12-550 Parkside Dr., Waterloo, Ontario N2L 5V4, Canada;
b Dept. of Biology, Wilfrid Laurier University, 75 University Ave. W., Waterloo, Ontario N2L 3C5, Canada

ABSTRACT

Conventional widefield light microscopy and confocal scanning microscopy have been indispensable for pathology and drug discovery research. Clinical specimens from diseased tissues are examined, new drug candidates are tested on drug targets, and the morphological and molecular biological changes of cells and tissues are observed. High throughput screening of drug candidates requires highly efficient screening instruments. A standard bio- medical slide is 1 by 3 inches (25.4 by 76.2 mm) in size. A typical tissue specimen is 10 mm in diameter. To form a high resolution image of the entire specimen, a conventional widefield light microscope must acquire a large number of small images of the specimen, and then tile them together, which is tedious, inefficient and error-prone. A patented new wide field-of-view confocal scanning laser imaging system has been developed for tissue imaging, which is capable of imaging an entire microscope slide without tiling. It is capable of operating in brightfield, reflection and epi-fluorescence imaging modes. Three (red, green and blue (RGB)) lasers are used to produce brightfield and reflection images, and to excite various fluorophores. This new confocal system makes examination of large biomedical specimens more efficient, and makes fluorescence examination of large specimens possible for the first time without tiling. Description of the new confocal technology and applications of the imaging system in pathology and drug discovery research,
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals
2. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
3. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
4. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
5. Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system
6. Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system
7. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
8. Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system
9. Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
10. A technique for increasing yields in bioreactors and disposable cell-culture systems
11. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
Post Your Comments:
(Date:7/30/2014)... (PRWEB) July 30, 2014 While ... in health services research for a relatively long ... in interest regarding the use of these ‘big ... conditions. , By combining today’s powerful computer technology ... digital era, ‘big data’ holds significant promise for ...
(Date:7/30/2014)... With the accelerated aging of ... expenditure as well as the improved medical care ... reagent industry has been developing quickly, especially in ... Chinese in vitro diagnostics market size reached RMB22.98 ... vitro diagnostic reagents. , Read complete report ...
(Date:7/30/2014)... AZ (PRWEB) July 30, 2014 ... State-of-the-art facility and complementary therapies brings comprehensive cancer ... foot, state-of-the-art Yuma Regional Cancer Center has officially ... 2012, the program includes services such as open, ... massage therapy, American Cancer Society Resource Center, hematology, ...
(Date:7/30/2014)...   Epic Sciences, Inc. ("Epic"), a private ... personalize and advance the treatment and management of cancer, ... as chairman of Epic,s board of directors. Mr. ... (formerly Invitrogen). Mr. Lucier joins Epic,s board of directors ... cell analysis platform with special focus on the development ...
Breaking Biology Technology:Leveraging ‘Big Data’ - Methodological Considerations in Health Services Research, New Webinar Hosted by Xtalks 2China Diagnostic Reagent Industry Report, 2013-2016 | Researchmoz 2China Diagnostic Reagent Industry Report, 2013-2016 | Researchmoz 3Sate-of-the-Art Yuma Regional Cancer Center Officially Opens 2Epic Sciences Appoints Greg Lucier as Chairman of the Board of Directors 2Epic Sciences Appoints Greg Lucier as Chairman of the Board of Directors 3
... the University of Helsinki, Finland, has succeeded in producing ... is reduced to a nano-scale (one nanometre being one ... undergo substantial changes. Unlike in bulk materials, in nanoparticles ... the number of atoms inside the material, which, among ...
... Corporation (Amex: ETC ) ("ETC" or ... will be exhibiting at the upcoming APWCA 2009 ... (APWCA) 2009 National Conference will be held April ... Philadelphia, Center City. The APWCA provides cutting-edge education ...
... Portfolio LAKE FOREST, Ill., March 16 ... equity firm focused exclusively on the healthcare ... CorePharma LLC ("CorePharma") has completed the acquisition ... Leucovorin Calcium tablets are the generic equivalent ...
Cached Biology Technology:Paper electrified by copper particles 2Environmental Tectonics Corporation Announces Participation at APWCA National Conference 2Environmental Tectonics Corporation Announces Participation at APWCA National Conference 3RoundTable Healthcare Partners Facilitates Product Acquisition for CorePharma 2
(Date:7/30/2014)... potential effects of climate change on the world,s ... wrong factors, according to a new paper by ... University of Queensland, and other organizations. The authors ... missing the point when it comes to climate ... change scientists focus on the "direct" threats of ...
(Date:7/30/2014)... Society of America (ESA) has elected ten new Fellows ... Fellow acknowledges outstanding contributions to entomology in one or ... The following Fellows will be recognized during Entomology 2014 ... held November 16-19, 2014 in Portland, Oregon. , ... the Department of Plant, Soil and Entomological Sciences at ...
(Date:7/30/2014)... joint venture between The University of Texas at San ... Center at San Antonio, has selected the winners of ... from UTSA and Lei Huang from the Health Science ... engineered ferritins for tracking and protecting neural stem cells ... focused on finding a treatment for stroke through stem ...
Breaking Biology News(10 mins):Conservation scientists asking wrong questions on climate change impacts on wildlife 2Entomological Society of America recognizes 2014 fellows 2Entomological Society of America recognizes 2014 fellows 3Entomological Society of America recognizes 2014 fellows 4Entomological Society of America recognizes 2014 fellows 5Entomological Society of America recognizes 2014 fellows 6Entomological Society of America recognizes 2014 fellows 7Entomological Society of America recognizes 2014 fellows 8Entomological Society of America recognizes 2014 fellows 9Entomological Society of America recognizes 2014 fellows 10Entomological Society of America recognizes 2014 fellows 11Entomological Society of America recognizes 2014 fellows 12Entomological Society of America recognizes 2014 fellows 13Entomological Society of America recognizes 2014 fellows 14Entomological Society of America recognizes 2014 fellows 15Entomological Society of America recognizes 2014 fellows 16Entomological Society of America recognizes 2014 fellows 17
... March 1 issue of G&D, Dr. Christopher Fasano (Memorial ... into the effective generation of neural stem cells outside ... demonstrates that the polycomb group protein, Bmi-1, maintains forebrain ... and embryonic forebrain neural progenitor cells increases stem cell ...
... since insects developed a taste for vegetation, plants have ... their neighbors for light to grow, or, invest directly ... of scientists at the Salk Institute for Biological Studies ... a la Agronoma (IFEVA) has discovered how plants weigh ...
... immunodeficiency virus (HIV) adapts so well to the body,s ... pace with the ever-changing immunological profile of the virus, ... Birmingham (UAB) and the University of Oxford in England. ... adapt by spelling out at least 14 different "escape ...
Cached Biology News:Understanding natural crop defenses 2Understanding natural crop defenses 3HIV adapts to 'escape' immune response 2
Request Info...
Form: Ready to use Applications: ELISA...
... supports that retain stiffness in liquids, protect ... segments on each strip have chemically bonded ... charts provide clear comparison. Full-range strips (0 ... packs of 100; each strip is approximately ...
... Bench-Top Open System Chemistry Analyzer for General Chemistries, Specialty Chemistries, QC, Reagent Development, Pharmaceutical ... grating, diode spectrophotometer over 30 user-selectable wavelengths. ... ... ...
Biology Products: