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A membrane-lid tube for bacteria cultivation and freeze-drying

tocols. It is possible to freeze-dry 500 l medium completely within 12 -13 hours and 200 l within 8 hours (see Fig. 3). Drying in the open standard tubes was quicker than in LidBac tubes. Discussion The experiments mentioned here were carried out to test the suitability of the membrane-lid tube LidBac both for bacteria cultivation as well as for freeze-drying.

The results show virtually identical growth behavior of the bacteria cultivated in LidBac in comparison to conventional cultivation methods in Erlenmeyer flasks. The same bacterial densities were achieved within the usual times. The slight concentration of the culture during incubation, caused by the vapor permeability of the membrane, had no adverse effect on the growth behavior of the bacteria.

The experiments showed that LidBac is also suitable for lyophilization. Although freeze-drying in the membrane-lid tube is slower than in open tubes, it still meets the requirements of standard protocols. It is possible to dry biological samples within one working day or overnight. The main advantage is the sterile condition achieved by the bacteria-proof membranelid when LidBac is used. Conversely, when open tubes are used, the chemical sterilization of the drying chamber of the freeze-dryer requires a lot of time and effort. For this reason, sterile conditions are rarely achieved in laboratory freezedrying units, and are not necessary when LidBac is used. Summary LidBac is a quick and easy way to eliminate several work steps, reduce the quantities of media required, save
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