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A membrane-lid tube for bacteria cultivation and freeze-drying

C over a period of 12 hours. The sample temperature was monitored continuously using a Pt100 sensor. After 4, 8,12, and 24 hours, the samples were removed from the drying process and the residual volume in the tubes was determined. The drying process had to be interrupted briefly each time the samples were removed. Results of bacteria cultivation Growth kinetics:
The time required for the cultivation of E. coli HB101 / B. subtilis 168 in LidBac corresponds to the usual times for 5 ml cultures in Erlenmeyer flasks (see Figs. 1 and 2). Undelayed growth was possible with both tube types and bacteria species up to an absorption of 1.8.

Sealed Safe-Lock microcentrifuge tubes (1.5 ml filled with 1ml culture) were selected as a negative control. The cell concentrations obtained in these tubes were much lower. Evaporation:
The assumption that evaporation phenomena are independent of the medium used (LB or water) was confirmed by the results of the growth of E. coli HB101 (results not shown).

Whereas there was virtually no evaporation with Erlenmeyer flasks that had been sealed with cellulose stoppers, the membrane-lid tubes displayed negligible evaporation values (< 7 % in 6 hours; approx. 20 % in 16 hours) (results not shown).

Cell morphology:
During both cultivation processes, no differences in cell morphology were noted with E. coli HB101 or with B. subtilis 168. Results of freeze-drying The speed at which solutions are freezedried in membrane-lid tubes corresponds to the requirements of standard pro
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