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A membrane-lid tube for bacteria cultivation and freeze-drying

ted in the Eppendorf Thermomixer R at 1,400 rpm and 37C. The Erlenmeyer flasks were sealed with cellulose stoppers and incubated in a linear oscillating water bath (Kttermann, approx. 200 strokes/min, at 37 C). The growth kinetics of the bacteria were determined by means of absorption measurements at 620 nm.

Evaporation:

Evaporation experiments were carried out with water since no significant differences were expected from experiments with culture medium containing growth.

1 ml water was transferred into each 1.5 ml LidBac tube. The tubes were sealed with their membrane-lid and then incubated in the Thermomixer R at 37 C and 1,400 rpm (room temperature 22-23 C, relative humidity 45-50 %). As a positive control, 5 ml water was transferred into each 50 ml Erlenmeyer flask, which was then sealed with a cellulose stopper and incubated in a linear oscillating water bath at 37 C. The weight of the tubes was determined in five parallel measurements, carried out after 3, 6, 9,18, and 24 hours. Cell morphology:

Since the cell morphology of bacteria often changes when insufficient amounts of oxygen are supplied for example, under sub-optimal air conditions, long, thin nutrient-deprived phenotypes are formed in bacteria that normally grow in short, rodlike shapes an analysis of cell morphology of cultivated bacteria enables the tubes to be assessed in regard to their suitability for bacteria cultivation.

Following overnight cultivation, the bacteria strains E. coli HB101 (facultatively
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