ted in the Eppendorf Thermomixer
R at 1,400 rpm and 37C. The Erlenmeyer flasks were sealed with cellulose
stoppers and incubated in a linear oscillating water bath (Kttermann,
approx. 200 strokes/min, at 37 C). The growth kinetics of the bacteria
were determined by means of absorption measurements at 620 nm.
Evaporation experiments were carried out with water since no significant
differences were expected from experiments with culture medium containing
1 ml water was transferred into each 1.5 ml LidBac
tubes were sealed with their membrane-lid and then incubated in the Thermomixer
R at 37 C and 1,400 rpm (room temperature 22-23 C, relative humidity
45-50 %). As a positive control, 5 ml water was transferred into each 50
ml Erlenmeyer flask, which was then sealed with a cellulose stopper and
incubated in a linear oscillating water bath at 37 C.
The weight of the tubes was determined in five parallel measurements,
carried out after 3, 6, 9,18, and 24 hours.
Since the cell morphology of bacteria often changes when insufficient amounts
of oxygen are supplied for example, under sub-optimal air conditions,
long, thin nutrient-deprived phenotypes are formed in bacteria that normally
grow in short, rodlike shapes an analysis of cell morphology of cultivated
bacteria enables the tubes to be assessed in regard to their suitability
for bacteria cultivation.
Following overnight cultivation, the bacteria strains E. coli HB101 (facultatively
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. A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase