The membrane-lid tube is suitable for virtually all stages of the experiment
whenever ventilation, drying, or a similar step is necessary. This is underlined
by a comparison of the results obtained by freeze-drying in LidBac
with those produced in a standard tube.
Material and methods
consists of a polypropylene (PP) membrane-lid with an
integrated PP hydrophobic membrane and a Safe-Lock microcentrifuge tube.
The membrane is gas-permeable, hydrophobic,and impermeable to bacteria (0.2
m pore size). The membrane lid is compatible with all 1.5 ml and
2 ml Eppendorf Tubes. After the tube has been filled, the membrane-lid
is simply pressed into the tube. As there is no hinged connection to the
tube, the lid can be removed easily at any time to enable the tube to be
used for follow-up applications.
Four parallel experiments were carried out in order to investigate the growth
behavior of Escherichia coli HB101 and Bacillus subtilis 168 when cultivated
. A positive control was carried out in parallel in
the form of bacteria cultivation in Erlenmeyer flasks. To ensure an identical
initial bacteria concentration in the different test tubes, LB (Luria Bertani)
medium was given a 1% inoculation of overnight culture. After being mixed
thoroughly, 1 ml bacteria culture each was transferred to 1.5 ml membrane-lid
tubes (Eppendorf LidBac
)and 5 ml each was transferred to a 50
ml Erlenmeyer flask with cellulose stopper.
The membrane-lid tubes were then incuba
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