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A membrane-lid tube for bacteria cultivation and freeze-drying

The membrane-lid tube is suitable for virtually all stages of the experiment whenever ventilation, drying, or a similar step is necessary. This is underlined by a comparison of the results obtained by freeze-drying in LidBac with those produced in a standard tube. Material and methods
LidBac
LidBac consists of a polypropylene (PP) membrane-lid with an integrated PP hydrophobic membrane and a Safe-Lock microcentrifuge tube. The membrane is gas-permeable, hydrophobic,and impermeable to bacteria (0.2 m pore size). The membrane lid is compatible with all 1.5 ml and 2 ml Eppendorf Tubes. After the tube has been filled, the membrane-lid is simply pressed into the tube. As there is no hinged connection to the tube, the lid can be removed easily at any time to enable the tube to be used for follow-up applications. Bacteria cultivation Growth kinetics:
Four parallel experiments were carried out in order to investigate the growth behavior of Escherichia coli HB101 and Bacillus subtilis 168 when cultivated in LidBac. A positive control was carried out in parallel in the form of bacteria cultivation in Erlenmeyer flasks. To ensure an identical initial bacteria concentration in the different test tubes, LB (Luria Bertani) medium was given a 1% inoculation of overnight culture. After being mixed thoroughly, 1 ml bacteria culture each was transferred to 1.5 ml membrane-lid tubes (Eppendorf LidBac)and 5 ml each was transferred to a 50 ml Erlenmeyer flask with cellulose stopper.

The membrane-lid tubes were then incuba
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