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A membrane-lid tube for bacteria cultivation and freeze-drying

Wilhelm Bockelmann, Institut fr Mikrobiologie, Bundesanstalt fr Milchforschung; Kiel, Germany
Susanne Wagner, Eppendorf AG Introduction Miniaturization plays an ever increasing role in everyday lab routines. Ongoing developments of tubes, pipettes, and pipette tips enable drastic reductions in the amount of material required. More economical use of reagents, the opportunity of using smaller sample quantities, and the elimination of bottlenecks thanks to reduced space requirements, save time and money and thus form the ideal prerequisites for increasing sample throughput.
The membrane-lid tube LidBac from Eppendorf is a significant contributory factor in the miniaturization process to the microbiological laboratory. For the first time ever, it is now possible to cultivate miniprep bacteria cultures in exactly the format required for the preparation. Following cultivation, the bacteria no longer have to be pipetted into a new tube since the culture is grown in the tube of the subsequent preparation. The automated production process enables the membrane-lid tube to be used directly, without need for prior sterilization. The cleaning process before and after cultivation, as required when conventional glass tubes are used, is no longer necessary. Target The experiments described in this article demonstrate the suitability of the membrane-lid tube LidBac for bacteria cultivation on a miniprep scale (1 to 2 ml). A comparison is made between the growth behavior of bacteria in LidBac and in conventional Erlenmeyer flasks.

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