Wilhelm Bockelmann, Institut fr Mikrobiologie, Bundesanstalt fr
Milchforschung; Kiel, Germany
Susanne Wagner, Eppendorf AG
Miniaturization plays an ever increasing role in everyday lab routines.
Ongoing developments of tubes, pipettes, and pipette tips enable drastic
reductions in the amount of material required. More economical use of reagents,
the opportunity of using smaller sample quantities, and the elimination
of bottlenecks thanks to reduced space requirements, save time and money
and thus form the ideal prerequisites for increasing sample throughput.
The membrane-lid tube LidBac
is a significant contributory factor in the miniaturization process to the
microbiological laboratory. For the first time ever, it is now possible
to cultivate miniprep bacteria cultures in exactly the format required for
the preparation. Following cultivation, the bacteria no longer have to be
pipetted into a new tube since the culture is grown in the tube of the subsequent
preparation. The automated production process enables the membrane-lid tube
to be used directly, without need for prior sterilization. The cleaning
process before and after cultivation, as required when conventional glass
tubes are used, is no longer necessary.
The experiments described in this article demonstrate the suitability
of the membrane-lid tube LidBac
for bacteria cultivation on a
miniprep scale (1 to 2 ml). A comparison is made between the growth behavior
of bacteria in LidBac
and in conventional Erlenmeyer flasks.
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