A Versatile Power Supply for All Electrophoresis Applications ( ty and resolution were obtai...)

A Versatile Power Supply for All Electrophoresis Applications

ty and resolution were obtained.

figure 4

Vertical Protein Gel Electrophoresis. The SmartPower 4000 power supply was used for protein gel electrophoresis in figure 4, which shows an image of an SDS-PAGE gel run at 130 volts (constant) for 1.5 hours. The SmartPower 4000 power supply has the low wattage control that is necessary to run protein gels. Typically, protein gels require low-wattage conditions (1 to 10 watts) because low-ionic-strength buffers are used.

figure 5

DNA Electroelution. Electroelution is a common technique for purifying DNA, RNA and protein from agarose and polyacrylamide gels. Stratagenes Quik-Pik electroelution capsules (figure 5) are designed for excising and eluting biomolecules from gel polymers. In figure 6, a Kb DNA Ladder molecular weight marker was electrophoresed through a 1% agarose gel in 1X TAE buffer and stained with ethidium bromide. The 2-kb band was excised from the gel and purified using the protocol provided with Quik-Pik electroelution capsules. The purified DNA band fragment was then electrophoresed next to an aliquot of the Kb DNA Ladder marker using the SmartPower 4000 power supply. The conditions for electroelution can be saved as individual files within the SmartPower 4000 power supply and readily recalled for a new experiment.

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