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A Versatile Power Supply for All Electrophoresis Applications

figure 2

DNA Sequencing. In figure 2, the SmartPower 4000 power supply was used in conjunction with the CastAway system and precast gels to obtain sequence from M13mp18 DNA template using the M13 (-40) primer. Sequencing results obtained using the SmartPower 4000 power supply and the CastAway system exhibit crisp, clear bands. Read lengths of approximately 400 bases per gel with double sample loading are routinely achieved (data not shown). The built-in temperature controller of the SmartPower 4000 power supply coupled with the thin, 0.25-mm format of the CastAway precast gels produce sequencing data of superior quality in a fraction of the time required for conventional sequencing methods.2 Due to the temperature controller of the SmartPower power supply, the maximum desired temperature of the gel can be set, eliminating the need for manual gel monitoring. This feature is especially useful when double loading sequencing gels since the longer run time can cause the gel to become hot, and the bands from the second set of samples to become diffuse (data not shown).

figure 3

Horizontal DNA Gel Electrophoresis. In figure 3, the SmartPower 4000 power supply was used for horizontal gel electrophoresis. The pBluescript vector was digested with EcoR I and serially diluted. These samples were loaded onto a 1% agarose gel and electrophoresed for 1.5 hours at 100 volts in 1X TAE buffer using the SmartPower 4000 power supply. Excellent band clari
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