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A Simple and Rapid Technique to Purify High-Quality Midiprep DNA

es endonucleases from the preparation, eliminating non-specific degradation of the plasmid DNA due to the bacterial strain used.

Pre-qualified pyrogen-free 50-ml conical tubes are also available for the preparation of endotoxin-free DNA for sensitive experiments. New plasticware is also considered endotoxin-free.

Conclusions

Use the fast, easy StrataPrep EF plasmid midiprep kit to isolate high-purity endotoxin-free plasmid DNA without resin slurries, toxic chemicals, or specialized equipment. With its convenient format, perform multiple preparations simultaneously, and generate yields similar to maxiprep scale purification. StrataPrep midiprep purified DNA results in accurate automated fluorescent sequence data with high intensity and low background, and the endotoxin-free DNA is transfected into mammalian cells with high efficiency.

REFERENCES

  1. Braman, J. and Basehore, S. (1997) Strategies 10: 84-86.

  2. Cotton, M., et al. (1994) Gene Therapy 1: 239-246.

  3. Pingerelli, P., Petre, M., and Karmiol, S. (1997) Strategies 10: 8-9.

  4. Stratagenes LipoTAXI Transfection Reagent Manual.

  5. Spector, D.L., Goldman, R.D., and Leinwand, L.A. (1998) In Cells: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. p. 86.2.


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