Navigation Links
A Simple and Rapid Technique to Purify High-Quality Midiprep DNA

eared bacterial lysate. A chaotropic salt is added to the lysate, and the mixture is transferred to a midispin cup. Upon centrifugation, plasmid DNA binds to the glass fiber matrix in the midispin cup. Contaminants are removed by washing the midispin cup, and the plasmid DNA is eluted in a small volume of buffer. No alcohol precipitation is necessary to concentrate the DNA. Endotoxin is efficiently removed by optionally extracting the cleared bacterial lysate prior to adding the chaotropic salt. In one hour, the standard midiprep plasmid purification is complete (only 1.5 hours with endotoxin removal); the standard anion exchange method takes a minimum of 3 hours to complete.

High Yields and Purity

Fig.2

The maximum binding capacity of a StrataPrep midispin cup is 350 g of plasmid DNA (Figure 2). The binding capacity of a single StrataPrep midispin cup was determined by loading various known quantities of purified pBluescript plasmid into midispin cups, then washing and eluting the DNA. The quantity of eluted DNA was measured by UV spectrophotometry. Nearly 350 g of high-copy number pBluescript II SK(+) plasmid DNA was also recovered from a 100-ml overnight bacterial culture using a single midispin cup, while the low-copy number pCMV gal plasmid yield from a 200-ml overnight culture was 250 g (Figure 3). Yields and spectrophotometric values (A260/A280 nm) between 1.8 and 2.0 were identical, using both the standard and endotoxin-free protocol (data not shown).

Figure 3

Quality Automated Sequencing

The quality of StrataPrep midiprep plasmi
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
4. Simple Isolation of RNA from Tissue and Cultured Cells
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
8. Simple Approaches for Optimization of RT-PCR
9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
11. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple and Rapid Technique Purify High Quality Midiprep DNA

(Date:8/28/2014)... Ind. A new research platform uses ... of tiny structures undergoing stress and heating, ... improve designs for microelectronics and batteries., This ... information about how heating and the surface ... properties. Researchers have discussed the merits of ...
(Date:8/28/2014)... SunTrust Robinson Humphrey (STRH) today announced the ... research team in biotechnology and oilfield services.  ... demonstrates our commitment to providing our valued institutional ... decision making," said Biff Woodruff , Head ... significant investments in differentiated content at a time ...
(Date:8/28/2014)... Vt. (PRWEB) August 28, 2014 ... testing around the world is available from Strategic ... diagnostics companies. “ Industrial Microbiology Market Review, Fourth ... Industrial Market ” (IMMR—4) tracks and compares microbiology ... in North America, Europe and Asia, and forecasts ...
(Date:8/27/2014)... 27, 2014 Varian Medical Systems today ... current Salt Lake City manufacturing facility and allow for ... to ultimately create 1,000 new full time jobs in ... “Utah is home to hardworking people with immense engineering ... with Varian’s success in the manufacturing of our state-of-the-art ...
Breaking Biology Technology:New analytical technology reveals 'nanomechanical' surface traits 2New analytical technology reveals 'nanomechanical' surface traits 3SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 2SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 32 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 3Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 2Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 3
... Corporation (NYSE: SPW ) today announced that ... a leading engineering company supplying processing solutions for the ... Auckland, New Zealand. Terms of the acquisition were not ... experience in the New Zealand dairy, beverage and bio-tech ...
... 9, 2011 Palatin Technologies, Inc. (NYSE Amex: ... ROTH 23rd Annual OC Growth Stock Conference on Tuesday, March ... be held at the Ritz Carlton Laguna Niguel in Dana ... Chief Executive Officer of Palatin Technologies, will provide an update ...
... AccessClosure, Inc., the U.S. market segment leader in ... first published study* (Journal of NeuroInterventional Surgery) comparing ... closure devices (VCD) as the primary endpoint.  The ... associated with the Mynx 5F Vascular Closure Device ...
Cached Biology Technology:SPX Announces Acquisition of B.W. Murdoch Ltd, Broadening Process Engineering and Project Management Capabilities in New Zealand and the Asia Pacific Region 2Significant Reduction in Pain With the Mynx Vascular Closure Device Versus Angio-Seal Device 2Significant Reduction in Pain With the Mynx Vascular Closure Device Versus Angio-Seal Device 3
(Date:8/28/2014)... 2014  Privacy Advocate and Senior Staff Attorney at ... joins the lineup of biometric and mobile commerce experts ... Executive Summit in Tampa, Florida ... speakers include Steven Rahman, Director, Technology and Strategy at Samsung, ... The theme of this year,s event is Mobility at ...
(Date:8/28/2014)... Bethesda, MD FASEB MARC (Maximizing Access to ... recipients for the American College of Sports Medicine,s ... 17-20, 2014 in Miami, Florida. These awards ... post doctorates and scientists from underrepresented groups into ... to encourage the participation of young scientists at ...
(Date:8/28/2014)... 2014A new method for measuring and imaging how quickly ... researchers better understand how drug abuse affects the brain, ... engineering, and lead to better treatment options for recovering ... of researchers from Stony Brook University in New York, ... published today in The Optical Society,s (OSA) open-access journal ...
Breaking Biology News(10 mins):Biometrics UnPlugged Welcomes the Electronic Frontier Foundation's Jennifer Lynch to the Mobility at the Crossroads of Commerce and Privacy Summit 2This is your brain's blood vessels on drugs 2This is your brain's blood vessels on drugs 3
... Tech researchers in computer science and biology have used ... algorithms that make it possible to simulate the cell ... have demonstrated that the new mathematical models and numerical ... going on inside living cells. , Biologist John Tyson, ...
... do not drill deep enough to find the living cells ... Mars, according to research led by UCL (University College London). ... once existed on Mars, cellular life could not survive the ... of Mars than a few metres deep ?beyond the reach ...
... and writing a letter whilst thinking about its content: ... This is possible due to the cerebellum. It regulates ... the cerebrum can perform other tasks. However, how the ... Angelique Pijpers reconstructed a part of cerebellar functioning in ...
Cached Biology News:Virginia Tech's System X supercomputer provides super tool for simulation of cell division 2Virginia Tech's System X supercomputer provides super tool for simulation of cell division 3Dig deeper to find Martian life 2Dig deeper to find Martian life 3On automatic pilot 2
... The Bio-Stack is compatible with ... systems. Speed, ease of use and ... routine microplate process. When used with ... reagent dispenser, the Bio-Stacks small footprint ...
... step in the evolution of laboratory automation. ... delivers labware to multiple laboratory instruments, LabLinx ... throughput of ordinary microplate robots. It allows ... configurations to meet any labs specific automation ...
... a fast and versatile automated strip washer ... Dual-ActionTM 16-channel manifold. This breakthrough design allows ... aspiration manifolds for overfill washing and overflow ... The problem of fitting dispense and aspirate ...
... potential applications for the use of small ... expression via the RNA interference (RNAi) pathway ... and molecular biology fields. siRNA for experimental ... chemically or enzymatically, and then transfected into ...
Biology Products: