Navigation Links
A Simple and Rapid Technique to Purify High-Quality Midiprep DNA


High-quality plasmid DNA for all demanding molecular biology applications

Scott Basehore Jeff Braman
Stratagene

A new technique is now available to quickly, easily, and reliably isolate high-purity plasmid DNA. The convenient spin-format eliminates the need for resin suspensions, gravity flow columns, and alcohol precipitations, while easily accommodating simultaneous multiple purifications. The purified DNA is ready for all applications, including sensitive automated sequencing and mammalian cell transfection.

StrataPrep midiprep kit purification of plasmid DNA1 is based on alkaline lysis of bacteria and binding of the liberated plasmid DNA to a glass fiber matrix contained in a midispin cup. Washing the spin cup removes contaminants, and the DNA is eluted in a small volume of buffer. The new midispin cup design and specially formulated alkaline lysis reagents allow up to 350 g of plasmid DNA to be recovered from a single device. The resulting DNA can be used for applications such as restriction digestion, cloning, PCR, site-directed mutagenesis, manual and automated fluorescent sequencing, and mammalian transfection.

Endotoxin, prevalent in plasmid preparations, is difficult to separate from plasmid DNA and results in toxicity to cultured mammalian cells.2 The StrataPrep EF midiprep protocol provides an optional extraction of endotoxins, which are reduced to 0.1 to 0.2 EU/g DNA (endotoxin units per microgram of plasmid DNA); hence, high-efficiency transfection results.

Fast and Easy Method

Fig.1

The StrataPrep midiprep procedure includes a unique color-indicating method that facilitates successful DNA purification (Figure 1). A modified alkaline lysis and centrifugation produces a cl
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
4. Simple Isolation of RNA from Tissue and Cultured Cells
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
8. Simple Approaches for Optimization of RT-PCR
9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
11. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple and Rapid Technique Purify High Quality Midiprep DNA

(Date:8/20/2014)... , Aug. 20, 2014 /PRNewswire-iReach/ -- A ... transformative collaboration between the school,s bioengineering department and ... of the Intel® Software Academic Program, UCSD,s research ... and the human body.  Photo ... study exhibits the work of Dr. Todd ...
(Date:8/20/2014)... 2014 GraphDB™ 6.0 ... improvements to the enterprise replication cluster, faster loading ... SOLR and Elasticsearch. This release happens to coincide ... triplestore – GraphDB™ was formerly known as OWLIM. ... only mature enterprise resilient RDF triplestore will also ...
(Date:8/19/2014)... Research and Markets  has announced the addition of the ... report to their offering. This report analyzes the ... by the following Product Segments: Media, Sera, and Reagents. ... Canada , Japan , Europe ... America . Annual estimates and forecasts are provided for the ...
(Date:8/19/2014)... DUBLIN , Aug. 19, 2014 Research ... Oxide Fuel Cells (SOFCs) - Global Strategic Business Report" ... the worldwide markets for Solid Oxide Fuel Cells (SOFCs) in ... US, Canada , Europe ... Annual estimates and forecasts are provided for the period 2012 ...
Breaking Biology Technology:Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4Global Cell and Tissue Culture Supplies - Strategic Business Report 2014 2Global Solid Oxide Fuel Cells (SOFCs) - Strategic Business Report 2014 2
... game of inches," but biology is a game of nanometers, ... the fate of a cell - whether it lives or ... Department of Energy (DOE),s Lawrence Berkeley National Laboratory (Berkeley Lab) ... impact of spatial patterns on living cells. Chemist Jay ...
... GAITHERSBURG, Maryland, November 2, 2011 - ... -based HPV Testing Increases Screening Coverage and is More Sensitive ... in the Lancet Promising findings published today ... community-based, randomized equivalence trial undertaken by public health authorities in ...
... Epitomics, Inc., a California based biotech company, announced ... Allowance from the United States Patent and Trademark ... Proteins (application 10/705,109).  The Notice allowed 17 claims ... samples using libraries of antibodies.  This method has ...
Cached Biology Technology:Berkeley Lab scientists develop new tool for the study of spatial patterns in living cells 2Berkeley Lab scientists develop new tool for the study of spatial patterns in living cells 3Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 2Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 3Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 4Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 5
(Date:8/19/2014)... Some infants born with neonatal abstinence syndrome ... more difficult time going through withdrawal than others, ... While genetic and epigenetic (when genes are turned ... as potential factors, researchers at Boston University School ... conducted a first of its kind study to ...
(Date:8/19/2014)... students in college biology and other science courses ... quantitative ways, and now they have a new ... the Life Sciences," published this month by Princeton ... Institute for Mathematical and Biological Synthesis (NIMBioS), teaches ... can be used to explore and explain biological ...
(Date:8/19/2014)... of UTSA researchers has been awarded a nearly $40,000 ... Training (NCPTT), an office of the National Park Service ... will fund a one-year study of the energy efficiency ... homes in hot, humid climates. , Radiant barriers are ... that reflect heat back, thereby reducing the amount of ...
Breaking Biology News(10 mins):In-utero methadone, Subutex exposure could alter gene expression, cause severe Neonatal Abstience Syndrome 2New textbook introduces undergraduates to mathematics for the life sciences 2UTSA researcher awarded $38K+ to evaluate energy efficiency options for historic homes 2UTSA researcher awarded $38K+ to evaluate energy efficiency options for historic homes 3
... certain disease mechanisms, illness progression and response to new ... such as nematodes, fruit flies or zebra fish. ... of many individual animals to detect mutants worthy of ... system that uses artificial intelligence and cutting-edge image processing ...
... scientists at the University of California, Berkeley, provide a clearer ... change, and where they go. One study, published ... Biology , finds that changes in precipitation have been underappreciated ... normal range. In the other study, published today (Wednesday, Aug. ...
... Mich. --- Two new species of owls have been discovered ... a key role in confirming their existence. The ... Forktail, the Journal of Asian Ornithology , took years to ... paper,s lead author Pam Rasmussen, MSU assistant professor of zoology ...
Cached Biology News:Artificial intelligence helps detect subtle differences in mutant worms 2Artificial intelligence helps detect subtle differences in mutant worms 3Artificial intelligence helps detect subtle differences in mutant worms 4Studies shed light on why species stay or go in response to climate change 2Studies shed light on why species stay or go in response to climate change 3Studies shed light on why species stay or go in response to climate change 42 new owls discovered in the Philippines 2