Navigation Links
A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

Diana Ji, Stacy L. Deeds, and Edward J. Weinstein


RNA interference methodologies have been utilized in small to large scale screening projects. The technology has allowed researchers to perform gene-silencing experiments in timeframes and target cells previously not possible. While siRNA screens have become fairly common, large scale screening with shRNA is still evolving. Advantages of shRNA-based experiments include long-term knockdown and viral delivery to non-transfectable cell types.

We set out to develop strategies for using lentiviral-based shRNA libraries in larger scale silencing projects (see Figure 1 for library vector map). Pilot screens using a tumor suppressor gene family set were performed to address biologically relevant questions while simultaneously developing screening strategies that could be used by a variety of researchers in the field.

A topic that has been extensively investigated is the effectiveness of chemotherapy for treating various carcinomas. A major cause of mortality in the United States, lung cancer, accounts for approximately one third of cancer-related deaths and has a low 5-year survival rate with few patients responding effectively to chemotherapy.

One potential treatment for lung carcinoma is paclitaxel. This anti-cancer agent (produced by Bristol-Meyers Oncology) functions by stabilization of microtubules so they cannot depolymerize. It effectively shifts the equilibrium in cells towards microtubule assembly, disrupting the normal operation of the microtubule network and thereby arresting the mitotic process.

Treatment with paclitaxel alone, however, is effective in only a fraction of the population. Approximately 21% to 24% of patients with non-small cell lung carcinoma (NSCLC) will respond to a regimen with this single agent. As a result, first line therapy may involve multiple chemothera
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Screening of Beer-Spoilage Bacteria Using the LightCycler PCR Workflow System
3. LightCycler foodproof Beer Screening Kit
4. Perform RNAi Library Screens on Any Budget
5. Recommendations for Successful siRNA Library Screens
6. Setting up Successful siRNA Library Screens
7. Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
8. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
9. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
10. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
11. A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT 8100 HTS System
Post Your Comments:
(Date:9/1/2015)... WI (PRWEB) , ... September 01, 2015 , ... In ... Venice expertly transports the reader to a not-so-distant future faced with an Ebola pandemic ... beautiful professional who overcomes personal tragedy to enter the world of counterterrorism, and who ...
(Date:9/1/2015)... ... 2015 , ... Knowledgent , the data and analytics firm, today announced ... data lakes in the healthcare industry. , As the Internet of Things (IoT) continues ... the last few years as more entities become technology enabled through Electronic Medical Records ...
(Date:9/1/2015)... ... September 01, 2015 , ... GenoCAD, a ... groundbreaking new version of its flagship DNA design platform, which has been enhanced ... sequences into numerous genetic parts, which in turn can be recombined using specific ...
(Date:8/31/2015)... SAN DIEGO , Aug. 31, 2015 ... drug development company announces that the Company,s lead antibody, ... subject of five separate presentations during the upcoming World ... Hawaii September 2-5, 2015. Researchers from the ... (MSK) will present results on the use of MabVax,s ...
Breaking Biology Technology:Alexi Venice’s Debut Novel Takes on the Human Testing of a Vaccine for Ebola and Revenge by an Anti-Big Pharma Terrorist Group in ‘Ebola Vaccine Wars' 2Alexi Venice’s Debut Novel Takes on the Human Testing of a Vaccine for Ebola and Revenge by an Anti-Big Pharma Terrorist Group in ‘Ebola Vaccine Wars' 3New White Paper Explores Data Lake Opportunities in the Healthcare Industry 2GenoCAD Releases Combinatorial Design Tool to Optimize Protein Expression and Streamline DNA Synthesis Orders 2MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 2MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 3MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 4
... 2012   Spherix Incorporated (NASDAQ: SPEX ... diabetes, metabolic syndrome and atherosclerosis, and provider of technical ... pharmaceutical companies, today announced that it has entered into ... its common stock and warrants to purchase shares of ...
... Cambrex Corporation (NYSE: CBM ) announced ... on Monday, February 6, 2012 after the market closes. ... call to discuss the fourth quarter 2011 financial results.  ... Tuesday, February 7, 2012 at 8:30 a.m. Eastern Time ...
... strength of spider silk, the robust nature of the ... tears and winds that exceed hurricane strength. Now, ... with complex computer simulations has shown that web durability ... the overall web design compensates for damage and the ...
Cached Biology Technology:Spherix Announces Registered Direct Offering of $1.15 Million 2Spherix Announces Registered Direct Offering of $1.15 Million 3A spider web's strength lies in more than its silk 2A spider web's strength lies in more than its silk 3A spider web's strength lies in more than its silk 4
(Date:8/20/2015)... , Aug. 20, 2015 The ... consumers that are active and healthy. However, wearable ... the hospital environment to help improve diagnostic capabilities ... video from the Wearable Technologies Conference 2015 ... new applications for wearables in healthcare.    ...
(Date:8/18/2015)... , Aug. 17, 2015 Research ... addition of the "Global Biometric Authentication & ... - Estimation & Forecast, 2015-2020" report to ... authentication and identification systems market is expected to ... 2015 to 2020 and generate over $25 billion ...
(Date:8/12/2015)... , Aug. 12, 2015  Synaptics Inc. (NASDAQ: ... interface solutions, today announced that four of its ... solution, have officially been named FIDO Certified™ by ... of the certification, Synaptics, Natural ID™ fingerprint solutions ... Authentication Framework (UAF) standard and are interoperable among ...
Breaking Biology News(10 mins):Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 2Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 3Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3
... 26 The Peter and Patricia Gruber Foundation International Prize ... with an event to be held at Rockefeller University in ... and Neuroscience Prize recipients will be announced live at the ... Brain, and Society." The discussion will feature a panel ...
... at the way that dolphins, whales and porpoises scythe ... maximum aquatic agility. However, no one had ever analysed ... lift that they generate, the drag that they experience ... from Duke University teamed up with Mark Murray from ...
... investigators in the Vanderbilt Center for Structural Biology have ... structure of the largest membrane-spanning protein to date. , ... pictures" of small proteins, large proteins and particularly ... been reluctant to smile for the camera. , In ...
Cached Biology News:NYC science symposium marks Gruber Prize program 10th anniversary 2Dolphins get a lift from delta wing technology 2Structural biology scores with protein snapshot 2Structural biology scores with protein snapshot 3
... , CapSure HS and CapSure Macro LCM ... of populations of pure cells from tissue ... Microdissection., Ensure the Integrity of Microdissected ... CapSure LCM Caps have a patented transfer ...
Opticon continuous fluorescence detector; includes optical base, analysis software, computer and monitor (this is an accessory for the DNA Engine thermal cyclers which must be ordered seperately)...
... on one plate. The new ArraySlide 96 ... (7mm x 7mm) individual printing surfaces for ... analysis studies. Unlike other systems, this device ... presence of contaminating adhesives. The chamber itself ...
... chambers for sealing hybridization reactions on slides. The ... on a glass slide. Reaction solution is added ... are then sealed with plastic sealing dots. Dots ... of reaction fluid. These chambers enclose a 20 ...
Biology Products: