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A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders

assware washing can contaminate samples, resulting in spurious peaks. The chromatographic process used in this method helped to prevent false attributions of VLCFA.) Finally, the method presented in this study was cost-effective, as a result of performing calibration using a homologue as an internal standard rather than using isotopically-labelled standards.

References

1D.W. Johnson, J. Inherit. Metab. Dis.(2000); 23:475-486.

Acknowledgements

Applied Biosystems/MDS SCIEX aknowledges our collaborators, Hlne Belva-Besnet and Bruno Casetta, and Maryse Gravier, Yves Tavet, Isabelle Cuvelier and Didier Olichon from Laboratoire PASTEUR-CERBA, Cergy (France) for providing the method, samples and data for this application note.


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