Navigation Links
A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders

ollision cell for multi-component analysis at reduced MRM dwell times, maintaining sensitivity and preventing cross talk. This reduces significantly the occurrence of false positive results.

Rugged and reliable triple quadrupole tandem MS system for maximum uptime

Compatibility with the broadest range of ionization sources for the analysis of a wide range of compounds.

Experimental Conditions

Samples were prepared according to the procedure described by Johnson(1) with some modifications. The hardware configuration included an API 3000TM LC/MS/MS System Triple Quadrupole Mass Spectrometer equipped with a TurboIonSpray source. The source operates in positive ion mode at a voltage of +5500 volts and with a turbo gas flow of 8 L/min of air heated at 300 degrees C (nominal heating-gun temperature).

MRM measurements were made using declustering potential (DP) and collision energy (CE) values as optimized automatically by the software for each of the analytes (DP value at 40 V; CE between 30 and 45 eV for the singly charged species measured here).

Chromatography was performed through an Agilent 1100 LC Binary Pump, and separation was accomplished via a Kromasil C8 5μm, 2 x 50 mm column housed in an Agilent 1100 Column Oven at 60C. 3 μL of the reconstituted sample extract were injected into the column using an Agilent 1100 Wellplate Autosampler. All hardware set-up details, solution and reagents requirements, and operating parameters were stored on CD for quick transfer of the proposed methodology.

Results and Discussion

The results of this research study are summarized in the Figures below.

As shown in Figure 1, chromatographic separation and identification of the derivatized pristanic, phytanic, docosanoic, tetracosanoic, and hexacosanoic VLCFAs, and the derivatized intern
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
3. Robust High Throughput RNA Isolation from Blood Samples
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using an Atmospheric Pressure Photoionization Source
6. HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer
7. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
8. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
9. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
10. Detection of nitrofurane metabolites in food with HPLC/MS/MS
11. Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS

Post Your Comments:
(Date:11/19/2009)..., , COPENHAGEN, Nov. 19 Dan... Inbicon cellulosic ethanol plant in Kalundborg, D... one of the world,s first and largest 2nd generati... Accellerase enzymes are used in the plant,s innov...or, a Division of Danisco, was the first to market...
(Date:11/19/2009)..., , BETHESDA, Md., Nov. 19 /PRNewswire-Fir...an innovator in biotechnology for diabetes therapy...lting services to food, supplement, biotechnology ...results of Tuesday,s Annual Meeting of Shareholder...ncorporated (Nasdaq: SPEX ) elected the following...
(Date:11/19/2009)..., World,s Top Gene Synthe...nsortium (IGSC) to Coordinate Best Practices in Ri... Percent of Worldwide Gene Synthesis Capacity , ...- Five of the world’s leading gene synthesis...l apply a common screening protocol to promote bio...
(Date:11/18/2009)..., , Security personnel need to be able to find ...ontact with them. To aid such searches, the Nation...ith support from the Department of Homeland Securi...al, Standard Reference Material (SRM) 2905, Trace ...laboratory assay methods, the SRM will be helpful ...
Breaking Biology Technology:DONG Energy and Inbicon Inaugurate One of the World's Largest Demo Plants for 2nd Generation Biofuel 2Spherix Announces Results of Annual Shareholders Meeting 2Spherix Announces Results of Annual Shareholders Meeting 3Spherix Announces Results of Annual Shareholders Meeting 4World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 2World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 3World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 4New NIST trace explosives standard slated for homeland security duty 2
...ulator that could also help expand the use of virt...monstrated on Wednesday at Chippewa Valley Techni...er, which was co-developed by the visual technolog...s lets students gain hours of welding experience ...llowing detailed critiques. , ,"In a nutshell, it ...
...a local software and consulting firm, has teamed u...w geographic information systems application, the ...ivX Integrator, allows organizations to create and...ork or the Internet. , ,"We understand the challen...distribute data from several different, separate d...
...of anti-spam equipment and services provided by Gr...r small businesses. MailFoundry Hosted starts at $...he service, a company would route its incoming e-m... then sent back to the company,s mail accounts. In...ilFoundry also offers a rack-mounted server that c...
Other Biology Technology:Virtual-reality welding simulator an opportunity for Chippewa Valley college 2
(Date:11/23/2009)...erman . , A bit of imagination on the part o...It could help to add data from areas where the ins...so constructively. In order to infer missing data ... imagination, physicists at the Max Planck Institu...atial perception called information field theory. ...
(Date:11/23/2009)...erman . , Of all global carbon dioxide emiss...where it contributes to global warming. The remain...stems such as forests, grasslands and peat-lands. ...restrial ecosystems is considered one of the main,...ange. However, new greenhouse gas bookkeeping has ...
(Date:11/22/2009)...ts have inventoried an astonishing abundance, dive...ve never known sunlight - creatures that somehow m...00 meters (~3 miles) below the ocean waves. , R...nguard technologies, animals known to thrive in an...rse collection of species ranging from crabs to sh...
Breaking Biology News(10 mins):Visual assistance for cosmic blind spots 2Visual assistance for cosmic blind spots 3Visual assistance for cosmic blind spots 4Intensive land management leaves Europe without carbon sinks 2Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 2Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 3Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 4Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 5Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 6Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 7Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 8Beyond sunlight: Explorers census 17,650 ocean species between edge of darkness and black abyss 9Mercy Health Plans Launches MercyOne Connect 28TM 29 49874 1Mercy Health Plans Launches MercyOne Connect 28TM 29 49874 2TriLink BioTechnologies Inc Announces Sales and License Agreement With Glen Research Corporation for CleanAmp 28TM 29 Amidites 49872 1TriLink BioTechnologies Inc Announces Sales and License Agreement With Glen Research Corporation for CleanAmp 28TM 29 Amidites 49872 2ChromaDex Establishes a Blanket Purchase Agreement With the National Institutes of Health 49869 1ChromaDex Establishes a Blanket Purchase Agreement With the National Institutes of Health 49869 2
... key compounds that stimulate stem cell growth in ...tion for people affected by Parkinson,s disease, s...urological disorders. These findings, which appear... provide important clues as to which compounds may...ns, to regenerate and ultimately restore brain fun...
...rs to prevent tuberculosis in cattle is unlikely t...odroffe, an ecologist at the University ofCaliforn...cientific Group on Cattle TB. , In Britain, farmin... for 30 years over the merits of culling badgers t...ccasionally be transmitted to people. Farming mini...
...men and children the hardest. A joint study betwee...versity in Uganda has now produced some important ...lf in the placenta. , Plasmodium falciparium is th...aria parasites that infect man. It is particularly... pregnant women, with fatal consequences for both ...
Other Biology News:British cattle give TB to badgers, finds UC Davis expert 2New findings could lead to vaccine for severe malaria 2
...ll; Reusable, press-to-seal gasket and cover slip...assembled for hybridizing and imaging thick or fre... 13 mm diameter. Build up to three chambers per sl...tion and leaking even when submerged. RNase-free, ...
Secure-Seal hybridization chambers from Sigma-Aldrich
LC-NMR-MSn system from Bruker Daltonics
...diagnostic assays, all EIA/RIA plates are manufact...iform binding, high optical clarity and low backgr...s are part of the Certified Surface Chemistry Prog...ce to be within QA release specifications. Linkage...
Biology Products: