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A Positive Selection Assay for Mutation Analysis in Big Blue,,,Animals

t-effective and less labor-intensive to perform due to its selective nature and small target region. This new assay can also serve to complement plaque color screening through mutation analysis of a second genetic region within the same animal. The l Select-cII mutation assay kit includes the G1250 selective host strain, enough Transpack lambda packaging extract** for 50 packaging reactions and lcII control mutants.

REFERENCES
  1. Mirsalis, J.C., Monforte, J.A., and Winegar, R.A. (1995) Annu. Rev. Pharmacol. Toxicol. 35: 145-164.

  2. Gorelick, N.J., and Mirsalis, J.C. (1996) Environ. Mol. Mutagen. 28: 434-442.

  3. Dycaico, M.J., et al. (1994) Mutat. Res. 307: 461-478.

  4. Jakubczak, J.L., et al. (1996) Proc. Natl. Acad. Sci. USA 93: 9073-9078.

  5. Schwarz, E., et al. (1978) Nature 272: 410-414.

  6. Place, N., et al. (1984) J. Mol. Biol. 180: 865-880.

  7. Rogers, B.J., et al. (1995) Mutat. Res. 327: 57-66.

  8. Young, R.R., et al. (1995) Mutat. Res. 327: 67-73.

* U.S. Patent No. 5,347,075 and patents pending. European Patent No. 289121, Japanese Patent No. 2618973
** U.S. Patent No. 5,188,957


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