Navigation Links
A Positive Selection Assay for Mutation Analysis in Big Blue,,,Animals

onditions (37C overnight) to determine the total number of rescued phage screened (figure 2, path B). Because the lLIZ shuttle vector contains the temperature-sensitive cI 857 mutation, both lcII + and lcII shuttle vector phages grow as plaques at 37C. Mutant frequency is determined as the number of lcII plaque-forming units (pfu) observed divided by the total pfu screened. The lcII selection scheme has been used to measure mutant frequency inductions in bladder tissue from mice treated with p-cresidine, with results comparable to lacI plaque color screening.4

The Big Blue Cyclist DNA sequencing kit can be used to rapidly sequence the lcII mutants identified with the l Select-cII assay. The cII target region is approximately 300 base pairs in length, including the cII mRNA ribosome binding site and the cII protein-activated PRE promoter.5,6 The relatively short length of the cII target region simplifies sequencing and expedites the analysis of mutational spectra.

Conclusions

The lSelect-cII mutation assay kit offers an alternative to the lacI plaque color-screening assay for mutation analysis in Big Blue transgenic rodents. Both assays feature unique advantages. The traditional plaque color-screening assay makes use of the larger, highly characterized lacI target region and benefits from the extensive database of experimental literature published over several years. By comparison, the lSelect-cII mutation assay is more cos
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
2. Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS
3. Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS
4. Plasmid Miniprep Kit Selection
5. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
6. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
7. Organize Your Data from the Big Blue Mutation Assay
8. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
9. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
10. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
11. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Positive Selection Assay for Mutation Analysis Big Blue Animals

(Date:9/23/2014)... (PRWEB) September 23, 2014 For the fifth ... Inc. 500|5000 list of the fastest-growing companies. , “We’re ... acknowledged yet again by Inc. Magazine,” ExakTime CEO Tony Pappas ... list by posting a three-year growth rate of 61 percent ... that time by more than 40 percent. , Pappas cited ...
(Date:9/23/2014)... 23, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the ... Q3 orders to date to $5 million. The orders are ... . "We continue to see new orders coming ... these orders, our backlog has climbed to over $11 million." ... a leading clean technology company that designs, manufactures and distributes ...
(Date:9/23/2014)... TORONTO , Sept. 23, 2014 /PRNewswire/ - Portage ... Canadian Securities Exchange: PBT.U), is pleased to announce ... (PPL) has added two more  consultants to its ... uveitis. PPL-003 uses a new proprietary cell permeable ... deliver its anti-inflammatory cargo into the eye. ...
(Date:9/23/2014)... CAMBRIDGE, Mass. , Sept. 23, 2014   ... developed a comprehensive suite of tools to enable the ... care, today announced that it has been awarded Phase ... Grant (SBIR) from the National Institutes of Health (NIH) ... biorepositories. Logo - http://photos.prnewswire.com/prnh/20140922/147649 ...
Breaking Biology Technology:ExakTime Makes Inc. 5000 List for the Fifth Time 2Portage Announces New Consultants for its PPL-003 Development Programs 2Portage Announces New Consultants for its PPL-003 Development Programs 3Portage Announces New Consultants for its PPL-003 Development Programs 4GenoSpace Awarded Fast Track SBIR Grant to Enable Data Access and Cohort Construction for Biorepositories 2
... ... biotechnology, ... Sciences today announced a partnership under which,Metahelix will assist in ... expert technological guidance,regarding usage. The efforts of this collaboration will ...
... Market Research, a,global provider of pharmaceutical competitive ... report providing critical strategic insight,for pharma and ... market for Rheumatoid,Arthritis (RA) therapies., In ... Analysis: Rheumatoid,Arthritis Q2 2008, MedPredict,s global Thought ...
... Arete Therapeutics Inc., a,privately-held biopharmaceutical company developing first-in-class ... of,James Sabry, M.D., Ph.D., as president and chief ... and member of the board of directors,since March ... position of,president and chief executive officer, who will ...
Cached Biology Technology:Sigma-Aldrich Announces Collaboration With Metahelix Life Sciences 2Sigma-Aldrich Announces Collaboration With Metahelix Life Sciences 3New MedPredict Report Highlights Trend Toward Early Diagnosis and Treatment of Rheumatoid Arthritis, Impact on Pharmaceutical Development 2Arete Therapeutics Names James Sabry, M.D., Ph.D., President and Chief Executive Officer 2
(Date:9/23/2014)... organisms, including humans, have parasitic DNA fragments called "jumping ... instructions in the process. And that phenomenon can result ... the University of Rochester now report that the "jumping ... when a multi-function protein stops keeping them in check ... In a study published today in Nature Communications ...
(Date:9/22/2014)... Yale University researchers are studying a potential new ... respiratory disease in which scars develop in the lungs ... microRNA mimic, miR-29, which is delivered to lung tissue ... fibrosis, it reversed fibrosis after several days. , ... EMBO Molecular Medicine . , "The mimic, when ...
(Date:9/22/2014)... by-products such as straw could be made quicker and ... UK and France. , Researchers funded by the ... variant straw plants whose cell walls are more easily ... significantly smaller or weaker than regular plants. , ... security as biofuels from non-food crops become easier and ...
Breaking Biology News(10 mins):A multi-function protein is key to stopping genomic parasites from 'jumping' 2Reversing the effects of pulmonary fibrosis 2Plant variants point the way to improved biofuel production 2
... protein 'wiring' that differs markedly from the cellular circuitry ... to the development of antimalarial drugs that exploit that ... parasite responsible for an estimated 1 million deaths per ... markedly from the cellular circuitry of other higher organisms, ...
... discovery in basic plant biology that may set the stage ... scientists have identified for the first time a protein that ... toxin to kill the cell. It had been known that ... plant cells, but never before has a protein been found ...
... University have successfully cultured in a laboratory a microorganism with ... advance that may ultimately help shed light on the ecology ... smallest free living cell known and probably the most abundant ... time to study the SAR11 "proteorhodopsin" gene in a laboratory, ...
Cached Biology News:Researchers develop new method to help find deadly malaria parasite's Achilles heel 2Researchers develop new method to help find deadly malaria parasite's Achilles heel 3Biologists discover new pathway into plant cells 2Biologists discover new pathway into plant cells 3Odd energy mechanism in bacteria analyzed 2