Navigation Links
A Positive Selection Assay for Mutation Analysis in Big Blue,,,Animals

providing the lacI plaque color-screening assay, Stratagene is now offering an alternative method of screening for mutations in Big Blue rodents, the l Select-cII mutation assay kit. In this positive selection assay, the region encompassing the cII gene of coliphage lambda functions as the mutational test sequence.4 The cII gene encodes a protein that activates transcriptional promoters in lambda that are essential for lysogenization. Mutations in the cII region that lower the levels of cII protein result in a decreased ability of lambda to lysogenize. When grown under conditions that favor lysogeny, lambda prophages carrying such mutations (lcII ) survive only by entering the lytic pathway of development, forming plaques. Prophages that are wild type for the cII region (lcII +) integrate into the host genome and become part of the developing bacterial lawn.

figure 2

The method of performing the l Select-cII mutation assay is outlined (figure 2). After rescuing the lLIZ shuttle vector from the rodent genomic DNA, the packaged virions are used to infect the E. coli host strain G1250 (a specialized hfl version of Stratagenes XL1-Blue MRA cells). The infected host cells are then plated and grown at 24C for 48 hours, conditions that are selective for lcII mutants (figure 2, path A). In addition, a dilution of infected G1250 cells is plated and grown under nonselective c
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
2. Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS
3. Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS
4. Plasmid Miniprep Kit Selection
5. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
6. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
7. Organize Your Data from the Big Blue Mutation Assay
8. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
9. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
10. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
11. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Positive Selection Assay for Mutation Analysis Big Blue Animals

(Date:9/20/2014)... (PRWEB) September 20, 2014 Entries are ... Prism Awards for Photonics Innovation . The international ... multi-billion dollar optics and photonics industry, and is sponsored ... photonics , and Photonics Media . , The ... versatility of photonics, said SPIE CEO Eugene Arthurs. , ...
(Date:9/20/2014)... HealthTronics, Inc. , a ... products and services, has signed a distribution agreement ... Corporation for its high precision laser technology. ... invasive, mobile, surgical technology—offering more choices for healthcare ... HealthTronics will make available Clinicon’s FDA-cleared SureLase™ CO2 ...
(Date:9/20/2014)... September 20, 2014 MediVet America ... perform a groundbreaking surgery at the specialized Wolvega Horse ... superficial digital flexor tendon was ruptured 90% and her ... injury is a death sentence for a horse. , ... stem cell treatments . This revolutionary regenerative option ...
(Date:9/19/2014)... Sept. 19, 2014  InterMune, Inc. (Nasdaq: ... Memorandum of Understanding to settle the litigation in the ... Plan of Merger dated as of August 22, 2014, ... Inc.  As one term of the Memorandum of Understanding, ... forth in the attached exhibit reflecting the amendment to ...
Breaking Biology Technology:Entry deadline is 10 October for international photonics Prism Awards 2Entry deadline is 10 October for international photonics Prism Awards 3Entry deadline is 10 October for international photonics Prism Awards 4HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 2HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 3World’s first tendon replacement using Nanofiber and activated MediVet Stem Cell Fraction 2World’s first tendon replacement using Nanofiber and activated MediVet Stem Cell Fraction 3InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 2InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 3InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 4InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 5InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 6InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 7InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 8InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 9InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 10InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 11InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 12InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 13InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 14InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 15InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 16InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 17InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 18InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 19
... 25 Scimitar Equity, LLC issues a ... VOQP), entitled,"Creating Value; Turnaround Leverages Product Opportunities ... review is available at website: http://www.scimitarequity.com ... Jersey-based biotechnology company,dedicated to becoming a recognized ...
... 25 Mymetics,Corporation (OTC: MYMX), will be holding an ... PM New York Time to update all shareholders,on the ... Form 10-Q and to,address recent press articles., You ... at +1-866-591-4361 PIN 815,726 and from Europe at +41-800-329-326 ...
... 23 Eisai Corporation of,North America and its ... Food and Drug Administration (FDA) has approved a ... prevention of,chemotherapy-induced nausea and vomiting (CINV). ALOXI Capsules ... prevention of acute nausea and,vomiting following initial and ...
Cached Biology Technology:VioQuest Pharmaceuticals, Inc Q2/08 Review Issued by Scimitar Equity, LLC 2FDA Approves ALOXI(R) (Palonosetron HCl) Capsules for Prevention of Acute Chemotherapy-induced Nausea and Vomiting 2FDA Approves ALOXI(R) (Palonosetron HCl) Capsules for Prevention of Acute Chemotherapy-induced Nausea and Vomiting 3
(Date:9/19/2014)... Durante of dinosaurs a newly discovered hadrosaur ... dinosaur, named Rhinorex condrupus by paleontologists ... University, lived in what is now Utah approximately ... period. , Rhinorex, which translates roughly into "King ... of other Cretaceous hadrosaurs like Parasaurolophus and Edmontosaurus. ...
(Date:9/19/2014)... researchers from several Harvard University labs in collaboration ... aspiring researchers with the intellectual raw materials needed ... soft, flexible materials. , With the advent of ... in manufacturing technology, soft robotics is emerging as ... conventional rigid robot design, but working with pliable ...
(Date:9/19/2014)... the unique bioelectric signaling system of the electric fish, ... The OU researcher is working to understand how these ... used to map the world around them. Electric ... discharges a second throughout their lives. The energetic ... necessary for survival. , "There is evidence that electric ...
Breaking Biology News(10 mins):New hadrosaur noses into spotlight 2Soft robotics 'toolkit' features everything a robot-maker needs 2
... Company Anticipates Acquisition Could More Than Double its Current ... the Medical and Healthcare Industry, SAN JOSE, Calif., ... a leading biometric and security,solutions provider, announced today the ... Medical Systems (OTC: CHSY), a company listed in,the US ...
... The gigantic fossil claw of an 390 million-year-old sea scorpion, ... insects, crabs and the like were surprisingly larger than ... O. Erik Tetlie , postdoctoral associate in the Department of ... of the report online in Royal Society Biology Letters. The ...
... Newly launched nerve cells in a growing embryo must chart ... use to navigate have yet to surface. In a study published ... Salk Institute for Biological Studies have recovered a key signal that ... spinal cord and must find their way down the length of ...
Cached Biology News:Titanium Group Signs Letter of Intent to Acquire Multimilion Dollar Medical Software Company and Its Existing Sales Network 2Titanium Group Signs Letter of Intent to Acquire Multimilion Dollar Medical Software Company and Its Existing Sales Network 3Titanium Group Signs Letter of Intent to Acquire Multimilion Dollar Medical Software Company and Its Existing Sales Network 4Salk scientists identify key nerve navigation pathway 2Salk scientists identify key nerve navigation pathway 3