Navigation Links
A Positive Selection Assay for Mutation Analysis in Big Blue,,,Animals


Cost-effective and easy-to-use l Select-cII mutation assay kit

A Positive Selection Assay for Mutation Analysis in Big Blue Animals

Mark J. Dycaico Gabriela M. Tobal
Stratagene

Stratagene now offers an alternative way to perform mutational analysis in the Big Blue transgenic rodent mutagenesis assay system that selects for mutations in the cII gene of coliphage lambda. The l Select-cII mutation assay kit uses an hfl E. coli strain and selective temperature conditions to provide an environment where only lambda cII mutants form plaques. This assay not only provides a less expensive means for studying mutations in Big Blue rodents* but also complements the original Big Blue (lacI) assay by focusing on a second genetic region for studying tissue-specific mutant frequencies and mutational spectra.

figure 1

Stratagenes Big Blue transgenic rodent mutagenesis assay system has been used extensively for studying mutations sustained in vivo in mammals.1,2 Big Blue transgenic rodents harbor the Big Blue lLIZ shuttle vector (figure 1), which can easily be rescued from the genome for mutation analysis in an E. coli host. In the established version of the Big Blue assay, the lacI gene from E. coli, located within the lLIZ shuttle vector, functions as a mutational test sequence. Rescued shuttle vector phage containing lacI mutations are scored as blue plaques against a background of colorless wild-type plaques.3

In addition to
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
2. Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS
3. Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS
4. Plasmid Miniprep Kit Selection
5. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
6. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
7. Organize Your Data from the Big Blue Mutation Assay
8. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
9. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
10. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
11. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Positive Selection Assay for Mutation Analysis Big Blue Animals

(Date:5/5/2015)... , May 5, 2015 Reprints Desk, ... in providing on-demand access to scientific, technical and medical ... with the Association of Southeastern Research Libraries (ASERL) ... research libraries located across 11 southeastern states. Founded in ... and collaborative programs to address the continually changing needs ...
(Date:5/5/2015)... D.C., USA (PRWEB) May 05, 2015 ... regulations covering a wide range of key photonics technologies ... hampering global competitiveness of U.S. industry. To help the ... webinar on 12 May on the proposed changes ... the commodities covered by International Traffic in Arms Regulations ...
(Date:5/5/2015)... 2015 Research and Markets ( ... "Clinical Laboratory, Molecular Diagnostics and Genomic Testing ... - Europe Version" report to their offering. ... area of molecular diagnostics and pharmacogenics hold the ... market that is moving out of the national ...
(Date:5/5/2015)... 5, 2015   Tocagen Inc. , a ... R. Parkinson , M.D., a venture partner at ... the board of directors of Tocagen. Dr. Parkinson ... oncology clinical development, including leading clinical activities at ... of the cancer therapeutics Gleevec®, Femara®, Zometa®, Kepivance® ...
Breaking Biology Technology:Association of Southeastern Research Libraries Partners with Reprints Desk to Improve Access for Hard-to-Find Materials 2Association of Southeastern Research Libraries Partners with Reprints Desk to Improve Access for Hard-to-Find Materials 3Association of Southeastern Research Libraries Partners with Reprints Desk to Improve Access for Hard-to-Find Materials 4Comments needed now from photonics industry on revisions of U.S. export rules 2Comments needed now from photonics industry on revisions of U.S. export rules 3Comments needed now from photonics industry on revisions of U.S. export rules 4European Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services Market 2015-2019 2Tocagen Appoints David R. Parkinson, M.D., to the Board of Directors 2Tocagen Appoints David R. Parkinson, M.D., to the Board of Directors 3Tocagen Appoints David R. Parkinson, M.D., to the Board of Directors 4
... 31, 2012  bioTheranostics, Inc., a bioMerieux company that ... treatment, has introduced a new product line of ... pathway interrogation called PRECIS Precision Medicine by bioTheranostics(SM). ... partners accurate diagnosis of primary tumor site and ...
... rap music can be harnessed to power a new type ... body. Acoustic waves from music, particularly rap, were found ... ultimately help to treat people stricken with aneurisms or incontinence ... a vibrating cantilever, a thin beam attached at one end ...
... The technique builds on previous research in which microbubbles were ... produce an oil which can be processed to create a ... important alternative to fossil fuels and algae, in particular, has ... now, however, there has been no cost-effective method of harvesting ...
Cached Biology Technology:bioTheranostics Launches Precision Medicine Biomarkers for Tumor Pathway Interrogation 2bioTheranostics Launches Precision Medicine Biomarkers for Tumor Pathway Interrogation 3Rap music powers rhythmic action of medical sensor 2Rap music powers rhythmic action of medical sensor 3Microbubbles provide new boost for biofuel production 2
(Date:4/9/2015)... , April 9, 2015 Synaptics Inc. (NASDAQ: ... announced today that it will report financial results for ... 23, 2015, after the close of market. The company ... investors at 2:00 p.m. PT (5:00 p.m. ET), during ... To participate on the live call, analysts and investors ...
(Date:4/2/2015)... , April 2, 2015 ... Q1 2015 attributed to somewhat higher than 125 MSEK. ... the year-end report 2014 that revenues for Q1 2015 ... the company reported for Q4 2014. The operating result ... be negative. The complete interim report will as previously ...
(Date:3/31/2015)... NEW YORK , March 31, 2015   ... announced the launch of the Post-Surgical Pain Management TRACKER, ... data products tracking usage trends in the medical device ... monthly access to data including treatment volumes, market share, ... manage the pain associated with joint surgery. ...
Breaking Biology News(10 mins):Synaptics to Report Third Quarter Results on April 23 2Fingerprint Cards Revises Revenues for Q1 2015 2Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3
... Nanyang Technological University (NTU),s Associate Professor Zhong Guofu ... organic chemistry, in particular the study of using small ... organocatalysis. Such synthesis process takes place for example, during ... Professor Zhong, who is from NTU,s School of Physical ...
... , , , , , ... , AUDIO: When soldiers leave base ... Click here for more information. , ... , , , , , ...
... you can against the surface. Now as hard as you ... Now faster. Now faster." These were the commands for ... ideas about mechanisms to control hand muscles. Complete understanding of ... vulnerable to aging and disease, and even help design more ...
Cached Biology News:NTU professor discovers method to efficiently produce less toxic drugs using organic molecules 2Army study improves ability to predict drinking water needs 2Army study improves ability to predict drinking water needs 3Army study improves ability to predict drinking water needs 4Army study improves ability to predict drinking water needs 51-finger exercise reveals unexpected limits to dexterity 21-finger exercise reveals unexpected limits to dexterity 3
...
... Major Science Major Science MS 300V (Cat. ... supply designed to meet most electrophoresis needs ... unit. Major Science MS 300V power supply ... to instruments used in electrophoresis. 4 pairs ...
...
The Programmable MP-3500P-F Power Supply can be applied for any Microfluidic application....
Biology Products: