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A Positive Selection Assay for Mutation Analysis in Big Blue,,,Animals


Cost-effective and easy-to-use l Select-cII mutation assay kit

A Positive Selection Assay for Mutation Analysis in Big Blue Animals

Mark J. Dycaico Gabriela M. Tobal
Stratagene

Stratagene now offers an alternative way to perform mutational analysis in the Big Blue transgenic rodent mutagenesis assay system that selects for mutations in the cII gene of coliphage lambda. The l Select-cII mutation assay kit uses an hfl E. coli strain and selective temperature conditions to provide an environment where only lambda cII mutants form plaques. This assay not only provides a less expensive means for studying mutations in Big Blue rodents* but also complements the original Big Blue (lacI) assay by focusing on a second genetic region for studying tissue-specific mutant frequencies and mutational spectra.

figure 1

Stratagenes Big Blue transgenic rodent mutagenesis assay system has been used extensively for studying mutations sustained in vivo in mammals.1,2 Big Blue transgenic rodents harbor the Big Blue lLIZ shuttle vector (figure 1), which can easily be rescued from the genome for mutation analysis in an E. coli host. In the established version of the Big Blue assay, the lacI gene from E. coli, located within the lLIZ shuttle vector, functions as a mutational test sequence. Rescued shuttle vector phage containing lacI mutations are scored as blue plaques against a background of colorless wild-type plaques.3

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