Navigation Links
A Positive Selection Assay for Mutation Analysis in Big Blue,,,Animals


Cost-effective and easy-to-use l Select-cII mutation assay kit

A Positive Selection Assay for Mutation Analysis in Big Blue Animals

Mark J. Dycaico Gabriela M. Tobal
Stratagene

Stratagene now offers an alternative way to perform mutational analysis in the Big Blue transgenic rodent mutagenesis assay system that selects for mutations in the cII gene of coliphage lambda. The l Select-cII mutation assay kit uses an hfl E. coli strain and selective temperature conditions to provide an environment where only lambda cII mutants form plaques. This assay not only provides a less expensive means for studying mutations in Big Blue rodents* but also complements the original Big Blue (lacI) assay by focusing on a second genetic region for studying tissue-specific mutant frequencies and mutational spectra.

figure 1

Stratagenes Big Blue transgenic rodent mutagenesis assay system has been used extensively for studying mutations sustained in vivo in mammals.1,2 Big Blue transgenic rodents harbor the Big Blue lLIZ shuttle vector (figure 1), which can easily be rescued from the genome for mutation analysis in an E. coli host. In the established version of the Big Blue assay, the lacI gene from E. coli, located within the lLIZ shuttle vector, functions as a mutational test sequence. Rescued shuttle vector phage containing lacI mutations are scored as blue plaques against a background of colorless wild-type plaques.3

In addition to
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
2. Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS
3. Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS
4. Plasmid Miniprep Kit Selection
5. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
6. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
7. Organize Your Data from the Big Blue Mutation Assay
8. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
9. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
10. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
11. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Positive Selection Assay for Mutation Analysis Big Blue Animals

(Date:1/15/2014)... , "The interface is the device," Nobel laureate ... properties to be found at the junctures where ... world of nanotechnology, the interfaces between layers of ... in such high-tech favorites as spintronics, high-temperature superconductors, ...
(Date:1/15/2014)... 15, 2014 Cynvenio, a cancer diagnostics ... the genomic analysis of tumor cells in the bloodstream, ... web-based cancer decision support application powered by CollabRx, Inc. ... ClearID™ genomic test, Therapy Finder will provide breast cancer ...
(Date:1/15/2014)... 2014 More than 5 million Americans ... in 3 seniors will die with Alzheimer’s or another ... figures have shocked many Americans into looking for ways ... these tragic age-related cognitive disorders. Jonathan Weisman, president of ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 Freeslate, ... solutions, today announced that Lupin Limited, one of India’s ... CM Protégé PharmD System for high throughput ... India, is focused on a wide range of quality, ...
Breaking Biology Technology:A deeper look at interfaces 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3
... Wis. - NameProtect , a Madison-based supplier ... Wednesday it has entered a partnership with Corillian ... state of Oregon. , ,The companies will jointly market ... streamlining and improving both companies' anti-fraud solutions. The pairing ...
... is taking the business world by storm, or so it ... outsourcing topics featured in business and IT publications and websites. ... two recently released studies on outsourcing published by leading consulting ... accentuate the negative aspects of this management practice. , ,Both ...
... Madison, Wis. - Alfalight, Inc. , a ... and telecommunications markets, has been awarded $1.4 million in ... Diode Sources program, sponsored by the Defense Advanced ... the project is to increase a laser's power conversion ...
Cached Biology Technology:Is outsourcing reaching its limits or simply pausing for breath? 2Is outsourcing reaching its limits or simply pausing for breath? 3Is outsourcing reaching its limits or simply pausing for breath? 4Alfalight garners $1.4 million defense funding for diode program 2
(Date:7/9/2014)... children with traumatic injuries have identified an immune ... develop a hospital-acquired infection. The study, led by ... in June in the journal Shock , ... lead to the clinical implementation of quick-turnaround immune ... immune system damage following critical illness or injury ...
(Date:7/9/2014)... Ill. With over 100 diseases that can attack ... top of the most wanted list? University of Illinois ... that more research is needed on the fungus that ... with cool, damp growing conditions but Macrophomina phaseolina ... and dry drought conditions. , "As the climate continues ...
(Date:7/9/2014)... Adlie penguins shows that the population is 3.79 ... previously estimated. Adlie penguins have long been considered ... the effects of climate change and fishing in ... imagery, researchers from Stony Brook University and University ... permits regular monitoring of Adlie penguins across their ...
Breaking Biology News(10 mins):Immune function predicts infection risk among child trauma patients 2Immune function predicts infection risk among child trauma patients 3Climate change provides good growing conditions for charcoal rot in soybeans 2Climate change provides good growing conditions for charcoal rot in soybeans 3New study finds that Adélie penguin population is on the rise 2New study finds that Adélie penguin population is on the rise 3
... CLEVELAND, Nov. 21, 2011 Already partnering with 43 ... world,s Top 100 Innovators, NineSigma today announced a record ... innovation is critical to their long-term growth. ... that never hesitate in their quest to innovate," said ...
... to measure their abundance has never been simple. A new ... and eight other scientists shows that expanding ocean dead zones ... wrinkle to that science. In the December 4 paper ... these scientists sound an alarm that expanding ocean dead zones ...
... Virginie Stevens ( CNRS ), Jean Clobert (CNRS), Michel ... show that interactions between dispersal and life-histories are complex, ... open access paper in the journal Ecology ... role in gene flow among populations, its evolutionary dynamics ...
Cached Biology News:Surging Demand for Innovation Services Leads to Record Expansion for NineSigma 2Surging Demand for Innovation Services Leads to Record Expansion for NineSigma 3Expanding dead zones shrinking tropical blue marlin habitat 2Expanding dead zones shrinking tropical blue marlin habitat 3Butterflies: 'Twice-punished' by habitat fragmentation and climate change 2