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A New Way to Assess siRNA Delivery Efficiency


Assess GAPDH siRNA delivery in less time for a fraction of the cost of real-time PCR

Analyze from one to 96 samples simultaneously

Measure both GAPDH siRNA-induced knockdown AND transfection-induced toxicity

Use with Silencer GAPDH siRNA Controls and Silencer CellReady Transfection Optimization Kit for a complete transfection optimization solution


Efficient and reproducible transfection is critical for any siRNA experiment. Transfection conditions should be optimized to provide the highest levels of gene silencing while minimizing toxicity associated with the transfection process. Ambions Silencer GAPDH siRNA is an ideal positive control for transfection optimization studies because it efficiently silences GAPDH--a ubiquitously and highly expressed gene--in human, mouse, and rat cells. Now Ambion has developed a new assay to rapidly measure this controls efficiency of delivery and induced level of GAPDH knockdown: the KDalert GAPDH Assay Kit.

Many researchers are currently using quantitative RT-PCR or Western blotting to assess siRNA-induced knockdown. While accurate, these techniques can be time-consuming and labor intensive. Another means to monitor siRNA delivery is use of fluorescently labeled siRNA. This method has the advantage of speed, but it can be unreliable since siRNAs can be trapped in endosomes or other sub-cellular compartments that keep them from directing target mRNA cleavage.


One Simple Assay for Two Critical Readouts<
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