| HOME >> BIOLOGY >> TECHNOLOGY |
ssDNA mutagenesis
yes
yes
yes
yes
T3/T7 RNA polymerase promoters
T7 only
yes
yes
yes
exo-mung deletions
no
yes
yes
yes
The Lambda ZAP vectors (Table
1) are available for prokaryotic expression (the Lambda ZAP II vector),
two-hybrid screening in yeast (the HybriZAP
2.1 vector), both prokaryotic and eukaryotic expression (the ZAP Express
vector), and now, the new Lambda ZAP-CMV vector. The Lambda ZAP II vector
is the most widely used vector for constructing cDNA libraries. Libraries
cloned in this vector can be functionally screened with nucleic acid probes
or antibodies. Alternatively, the library can be quickly and easily mass
excised into the pBluescript SK(-) plasmid vector for analysis
of transcripts via expressed sequence tag (EST) sequencing or subtraction
and normalization procedures. The HybriZAP 2.1 vector offers the convenience
of lambda library technology; after library construction, excision of
the pAD-GAL4-2.1 plasmid vector allows cloned DNA to be screened for protein-protein
interactions in vivo. The ZAP Express vector offers library construction
with the capability for both prokaryotic a
'"/>
Source:
Related biology technology :
1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Lambda DNA Extraction Protocol
3. Lambda DNA Extraction Protocol
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
| Copyright © 2003-2012 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |