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A New Lambda Vector for Mammalian Expression

to CHO cells and selected cells with G418 to establish a stable transfected cell line with high levels of luciferase expression (data not shown). These data confirm that the pCMV-Script-EX phagemid vector expresses high levels of protein in mammalian cells.

Lambda ZAP Vectors for Diverse Applications

Table 1
Stratagenes Lambda ZAP cDNA Library Vectors

Comparison of Lambda cDNA Library Vectors

HybriZAP 2.1
two-hybrid vector

ZAP Express
vector

Lambda ZAP-CMV
vector

Lambda ZAP II
vector

# unique cloning sites in l
(# sites in excised plasmid)

2
(7 in pAD-GAL4-2.1)

12
(17 in pBK-CMV)

3
(15 in pCMV-Script)

6
(21 in pBluescript SK- phagemid)

cloning capacity

6 kb

12 kb

6.5 kb

10 kb

eukaryotic expression

yes
(pADH1 promoter)

yes

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Source:


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