Navigation Links
A New Lambda Vector for Mammalian Expression

restriction sites in the multiple cloning site, and the pCMV-Script-EX phagemid vector.

Figure 2

The pCMV-Script-EX vector contains 15 unique sites for cloning (Figure 2) and offers the same features as its parental pCMV-Script vector;6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines;### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b-lactamase promoter, to provide kanamycin resistance in bacteria, as well as the SV40 early promoter, to provide G418 resistance in mammalian cells. The pCMV-Script-EX vector varies from its parental pCMV-Script vector by a 29-bp sequence downstream of the f1 origin; however, this additional sequence is in a nonfunctional region of the vector. Thus, the new pCMV-Script-EX vector is functionally equivalent to the pCMV-Script vector.

The Lambda-ZAP CMV vector is available in kits of varying components to accommodate different research needs. The Lambda ZAP-CMV XR library construction kit includes the directional cDNA synthesis kit, the Lambda ZAP-CMV XR vector, Gigapack III Gold packaging extract, host strains, and helper phage. Lambda ZAP-CMV vector kits are available that include the Lambda ZAP-CMV vector predigested with either EcoR I only or EcoR I and Xho I, host strains, and helper phage. In addition, the Lambda ZAP-CMV vector is available in undigested form with host strains and helper phage.

cDNA Library Construction in the Lambda ZAP-CMV Vector

Figure 3

Libraries constructed in the Lambda ZAP-CMV vector are characterized by high titers and robu
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Lambda DNA Extraction Protocol
3. Lambda DNA Extraction Protocol
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Lambda Vector for Mammalian Expression

(Date:6/16/2013)... This training is ideal for those who will ... software industry or those who want to explore Agile and ... The topics that will be covered in the 1-day Agile ... scrum artifacts, and scrum simulation (optional). , The trainings ... June 28, 2013 , Click here to register for a ...
(Date:6/14/2013)... June 14, 2013 These couplings are ... come equipped with a new and improved “Easy Grip ... more secure grip and reliable connection every time. , ... for use on medical applications such as ... pressure cuff manufacturers with better, more reliable, high quality, ...
(Date:6/14/2013)... Rosacea is said to affect 16 million Americans, eczema, ... it seems that most of the population have one or ... reports, often deciphering the difference between one skin condition ... have similar symptoms. Probiotic Action explains how these skin conditions ... effectively. , Rosacea, Acne and Eczema are conditions that react ...
(Date:6/14/2013)... , June 14, 2013  The Pittsburgh Life Sciences ... levels of investment that were necessitated by delays in ... investment climate and the uncertainty of state funding. ... said, "As the region,s only pure life sciences investment ... by the PLSG Board of Directors, were necessary to ...
Breaking Biology Technology:Affordable Scrum Trainings in Denver, CO – 8 PDUs (June 28, 2013) by Conscires Agile Practices 2LinkTech Inc. Launches Ideal Replacement for a Luer Fitting 2LinkTech Inc. Launches Ideal Replacement for a Luer Fitting 3Rosacea, Acne, or Eczema? Probiotic Action Offers a Topical Probiotic to Treat the Most Common Skin Conditions 2Pittsburgh Life Sciences Greenhouse Makes Significant Reductions Due to Uncertain Funding 2
... ... investment bank KTA Capital,s client Neuren Pharmaceuticals Ltd (ASX:NEU) has agreed an AU$6.7 mil ... by KTA Capital as Neuren,s exclusive placement agent and advisor. , ... New York (PRWEB) November 23, 2009 ...
... ... field remains the only one to show consistent growth during the U.S. recession. However, clinics ... actually cut medical personnel, instead of just administrators. , ... (Vocus) November 24, 2009 -- Health care employment continued growing in ...
... 24 Blue Marble Energy Corp. announced today that ... were recently awarded $2 million by Washington State,s Community ... construct the state,s first specialty biochemical biorefinery in Lincoln ... speed the development of our biorefinery and bring over ...
Cached Biology Technology:KTA Capital Arranges an AU$6.7 Million Private Placement by Neuren Pharmaceuticals 2The MedZilla Report for October 2009 - Health Care Employment Grows Again in October Even As Clinics, Specialty Centers Close 2The MedZilla Report for October 2009 - Health Care Employment Grows Again in October Even As Clinics, Specialty Centers Close 3The MedZilla Report for October 2009 - Health Care Employment Grows Again in October Even As Clinics, Specialty Centers Close 4Blue Marble Energy, OPDA Awarded $2M by WA's Community Economic Revitalization Board 2
(Date:6/16/2013)... HIV prevention programs for female sex workers in India ... infections (STIs), a University of Toronto study has found. ... with HIV, mostly in the southern states of Andhra ... by Professor Prabhat Jha from U of T,s Dalla ... Centre for Global Health Research (CGHR), examined the impact ...
(Date:6/16/2013)... snap" 116 million years ago triggered a similar marine ... a result of global warming, according to research published ... involving experts from the universities of Newcastle, UK, Cologne, ... and a crash in the marine ecosystem during the ... the first time the amplitude and duration of the ...
(Date:6/14/2013)... Oklahoma researchers will collaborate with researchers from Oklahoma ... the University of Tulsa to advance understanding of ... climate change and variability in the state. , ... receive a $20 million National Science Foundation Experimental ... strategically aligned, innovative research over the next five ...
Breaking Biology News(10 mins):HIV prevention among female sex workers in India reduces HIV and syphilis 2Global cooling as significant as global warming 2OU researchers collaborate on $20 million NSF EPSCoR grant 2OU researchers collaborate on $20 million NSF EPSCoR grant 3
... An international team of scientists, including botanists from the University ... be used to catalogue the world,s plants using a technique ... method that uses a short genetic marker in an organism,s ... "Barcoding provides an efficient means by which we can ...
... promising data from a clinical study showing patient-specific ... and immune cells were well tolerated and resulted ... metastatic melanoma whose disease had been minimized by ... "Phase II Trial of Dendritic Cells Loaded with ...
... Mitochondrial diseases disrupt the power generating machinery within cells ... in the lungs or respiratory tract. A new study ... shows that infection with the pneumonia causing bacteria ... a signaling protein that is associated with mitochondrial disease. ...
Cached Biology News:University of Toronto helps to 'barcode' the world's plants 2Study shows cancer vaccines led to long-term survival for patients with metastatic melanoma 2Stress signals link pre-existing sickness with susceptibility to bacterial infection 2
Immunogen: Synthetic peptide (aa sequence is considered to be commercially sensitive) Storage: -20 C, Avoid Freeze/Thaw Cycles...
...
TRIzol Reagents are ready-to-use monophasic solutions of phenol and guanidine isothiocyanate suitable for isolating total RNA DNA and proteins (16)....
...
Biology Products: