A New C-Terminal GST Vector for Protein Production in S. pombe ( gene was expressed using the pESP-3...)

A New C-Terminal GST Vector for Protein Production in S. pombe

gene was expressed using the pESP-3 vector. The luciferase-GST fusion protein was purified to near homogeneity with a yield of 12.5 mg/liter (data not shown).

Stratagenes FLAG Western Detection Kit can be used to monitor expression and purification processes. With this kit, recombinant fusion proteins that are tagged with the FLAG epitope at N-terminal, C-terminal and internal positions can be detected. As shown in figure 3, the FLAG epitope contained in the calmodulin-GST fusion protein can be detected before and after purification of the fusion protein.

figure 3

Other pESP Vectors

figure 4

Stratagene previously introduced the pESP-1 and pESP-2 vectors (figure 4), which feature N-terminal tagging with GST for purification.^2-4 In addition, the pESP-2 vector offers the option of cloning PCR products through ligation-independent cloning (LIC).¹² When the LIC method is used, polypeptides without extraneously added amino acid residues can be obtained after removing the GST tag by enterokinase cleavage.^3,4 Both the pESP-1 and pESP-2 vectors are available separately and as components of the ESP system and ESP LIC systems, respectively. The ESP system has been extensively tested for inducible expression of a variety of eukaryotic proteins, including human MEK kinases and rat c-Jun N-terminal kinase (JNK). In these tests, up to 12.5 mg/liter of recombinant proteins has been obtained (table 1).

<
'"/>

Source:

Page: All 1 2 3 4 5 6 7 8 9
Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. A New Lambda Vector for Mammalian Expression
9. Versatile Reporter Vectors for Monitoring Viral Transduction
10. High-Titer Retroviral Vectors for Gene Delivery
11. Improved Vectors for PathDetect Trans-Reporting Systems

Post Your Comments:

( gene was expressed using the pESP-3...)A New C-Terminal GST Vector for Protein Production in S. pombe

figure 3

Other pESP Vectors