Navigation Links
A New C-Terminal GST Vector for Protein Production in S. pombe

the GST gene. Cleavage of the fusion protein with enterokinase results in a protein with the FLAG epitope tag attached on its C-terminus; cleavage by thrombin removes both the GST sequence and the FLAG epitope.

Expression and Purification of Chicken Calmodulin

The chicken calmodulin gene was inserted into the Nde I and BamH I sites of the pESP-3 vector, serving as a test gene for protein expression and purification. The calmodulin gene primers were designed such that the calmodulin gene would be fused in frame at its C-terminus to the GST gene. Recombinant clones were identified by colony PCR analysis using vector-specific primers (the pESP-3 vector forward and reverse primers) that flank the MCS. A positive clone was chosen for further study.

Figure 2 Panel A

figures 2 Panel B

figures 2 Panel C

The pESP-3 vector containing the chicken calmodulin gene was transformed into the S. pombe strain SP-Q01 using a standard protocol.11 Figure 2 Panel A shows the expression of the calmodulin-GST fusion protein and purification by one-step chromatography using the glutathione-agarose beads1 contained in the ESP yeast protein expression and purification kit. The level of induced expression of the calmodulin-GST fusion protein is estimated to be 10% to 15% of the total soluble protein. Approximately 12.5 mg of the calmodulin-GST fusion protein was obtained from 1 liter of induced culture. After purification of the fusion protein, the GST tag was removed by incubating the fusion protein with either enterokinase or thrombin (figures 2 Panel B and Panel C). In similar experiments, the firefly luciferase
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. A New Lambda Vector for Mammalian Expression
9. Versatile Reporter Vectors for Monitoring Viral Transduction
10. High-Titer Retroviral Vectors for Gene Delivery
11. Improved Vectors for PathDetect Trans-Reporting Systems
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Terminal GST Vector for Protein Production pombe

(Date:12/19/2014)... 18, 2014  Decision Resources Group finds that the ... will grow moderately through 2023 as the aging population, ... of dental biomaterials all spur procedure volumes. Growth will ... China and India ... within reach for a larger proportion of the population. ...
(Date:12/19/2014)... 18, 2014   ISN has released its ... Indicators publication series. With data aggregated from ... in 2013 within its online contractor management platform, ISNetworld, ... performance indicator (KPI) statistics. The ... publication is available in two industry specific volumes: ...
(Date:12/17/2014)... New York, NY (PRWEB) December 16, 2014 ... undergone significant changes since 2013, which is why IBISWorld ... one, this industry continues to benefit from an intensified ... 2014, the proposal for new emission standards for power ... affected industry operators. According to IBISWorld Industry Analyst Sarah ...
(Date:12/17/2014)... Bellingham, Washington, USA (PRWEB) December 16, 2014 ... Kirstin Baum, manager at Philipps- Universität Marburg’s Department ... optics and photonics, in the newly released ... 2015-2016. “And never be too shy to ask ... STEM (science, technology, engineering, and mathematics) occupations ranging ...
Breaking Biology Technology:Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 3ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 3Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 2Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 3Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 2Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 3
... molten steel as the representative symbol of a growing ... cubicle farm. Instead of coke and iron ore, information ... and information into knowledge and ideas: An invisible production ... is knowledge. Every physical product has a knowledge component ...
... manufacturer of diode lasers, announced today the appointment of ... executive officer. Warrior brings Fortune-50 semiconductor manufacturing and operational ... company. , , Mr. Warrior is an experienced ... Hoke , chairman of the board for Alfalight. interim ...
... that allow AIDS viruses to escape detection by the ... grow after transmission to new hosts, scientists at the ... Nature Medicine. , ,The discovery may help researchers design ... training the immune system to attack the virus where ...
Cached Biology Technology:Transforming Information to Knowledge 2Studies Offer New Insight into HIV Vaccine Development 2
(Date:12/10/2014)... Dec. 9, 2014  Wake Forest Baptist Medical Center today ... its School of Medicine. Funding for this $50 million ... that will be publicly launched next summer. ... the former 60 series R.J. Reynolds Tobacco Company complex, ... will begin immediately with plans to be ready to ...
(Date:12/5/2014)... -- Tute Genomics, a leader in cloud-based solutions for genome ... led by UK-based Eurovestech. Peak Ventures and a number ... "We are at a pivotal time in ... seeks new approaches for the diagnosis, treatment and prevention ... and CEO of Tute Genomics. "One of the main ...
(Date:11/21/2014)... -- Earlier this year Donald Spector , Chairman of ... the most prolific inventors in the world, got a ... to third party agencies. Spector envisioned this technology even ... earliest known patents in this area. Now major technology ... care, elder care and hospital applications. This technology has ...
Breaking Biology News(10 mins):Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4
... December 15, 2009 Signal Hill, CA Tom ... public and president of Bowman Global Change, has developed ... the fourth Intergovernmental Panel on Climate Change (IPCC) report ... to the authentic scientific information they need to make ...
... , REDWOOD CITY, Calif., Dec. ... and authentication solutions, today announced an agreement with IBM ... fingerprint technology on IBM SurePOS 500 retail systems. The ... easily and quickly identify users so that specific transactions ...
... ... Dec. 8, 2009 National Instruments (Nasdaq: NATI ), ... a leader and pioneer in manufacturing automation using robotics technology, ... technology with DENSO robotic arms. The collaboration increases productivity and ...
Cached Biology News:Bowman creates graphic translation of climate change data 2DigitalPersona Fingerprint Sensor Technology Ships on IBM SurePOS 500 Retail Systems 2National Instruments and DENSO Robotics Collaborate to Address New Applications in Industrial Robotics 2National Instruments and DENSO Robotics Collaborate to Address New Applications in Industrial Robotics 3National Instruments and DENSO Robotics Collaborate to Address New Applications in Industrial Robotics 4National Instruments and DENSO Robotics Collaborate to Address New Applications in Industrial Robotics 5