Navigation Links
A New C-Terminal GST Vector for Protein Production in S. pombe


Versatile eukaryotic expression and protein purification vector

Quinn Lu Tanya Hosfield
Stratagene

Stratagene has constructed and tested the new pESP-3 vector, which can be used for C-terminal fusion with glutathione-S-transferase (GST) and for protein production in Schizosaccharomyces pombe. After expression and purification of the GST fusion protein using the ESP yeast protein expression and purification system, the GST tag can be removed by proteolytic cleavage with thrombin or enterokinase.

Expression and purification of heterologous genes often involve fusion of the gene of interest with a purification tag at either the N-terminus or C-terminus. The Schistosoma japonicum GST gene product has been popularly used as a protein fusion tag for affinity purification of GST fusion proteins.1 Stratagenes ESP yeast protein expression and purification system includes the pESP-1 and pESP-2 vectors for N-terminal tagging with GST.2-4 The fission yeast S. pombe, which is used in the ESP system, possesses characteristics that closely resemble those of higher eukaryotic organisms regarding chromosome structure and function, cell cycle control and RNA splicing.5 Stratagenes ESP system features high-level protein production in S. pombe, thereby retaining the posttranslational modifications of eukaryotic proteins that may be critical for their structure and function.

The availability of an expression vector for C-terminal tagging with GST is desirable since the stability and activity of fusion proteins may vary with the location of the tag.6-8 In addition, by tagging a protein at the C-terminus, only fully translated proteins will have the GST affinity tag, ensuring purification of full-length fusion proteins. Stratagene has constructed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. A New Lambda Vector for Mammalian Expression
9. Versatile Reporter Vectors for Monitoring Viral Transduction
10. High-Titer Retroviral Vectors for Gene Delivery
11. Improved Vectors for PathDetect Trans-Reporting Systems
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Terminal GST Vector for Protein Production pombe

(Date:12/24/2014)... Dec. 24, 2014  United Therapeutics Corporation (NASDAQ: ... MDT ) has submitted a pre-market approval application ... the use of Medtronic,s SynchroMed ® II implantable ... use with United Therapeutics, Remodulin ® (treprostinil) Injection ...
(Date:12/24/2014)... 2014  Vermillion, Inc. (Nasdaq:   VRML), a bio-analytical ... that the Company closed its previously announced private ... Jack W. Schuler , Birchview Fund LLC and ... before offering expenses.  The proceeds will be used ...
(Date:12/24/2014)... December 23, 2014 “Preparative & ... HPLC, Flash), Consumables (Columns, Solvents, Buffers, Valves, Guages, ... & Agriculture) - Forecasts to 2019” provides a ... opportunities, current market trends, and strategies impacting the ...
(Date:12/24/2014)... Boston, MA (PRWEB) December 23, 2014 Earlier ... James L. Sherley, director of the Adult Stem Cell Technology ... overlooked and under appreciated unique property of adult tissue stem ... Misunderstood in the Past, Important for the Future,” embodied the ...
Breaking Biology Technology:United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 3United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 4Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4
... 2011  Synergy Pharmaceuticals, Inc. (Nasdaq: SGYPD, SGYPU), a developer of ... today that it has filed a lawsuit in New York ... IRWD ) and its Chief Scientific Officer and Senior VP ... lawsuit alleges that after European patent 1,379,224 was granted to ...
... (Nasdaq: SGMO ) announced the presentation of new ... in an adult mouse model of hemophilia B using systemic ... Meeting of the American Society of Hematology (ASH). ... the clotting factor, Factor IX, with a single, systemic administration ...
... The Pharmabiotic Research Institute (PRI) brings together industrial and ... is a unique scientific and regulatory association in Europe. ... traditional food processing applications, probiotics can also be active ... why PRI uses the terminology "Pharmabiotics", relating to "LBP ...
Cached Biology Technology:Synergy Pharmaceuticals, Inc. Files Suit Against Ironwood Pharmaceuticals, Inc. and its Chief Scientific Officer and Senior VP of R&D, Dr. Mark G. Currie 2Sangamo BioSciences Announces Presentation of New Data from ZFP Therapeutic® Program in Hemophilia B at American Society for Hematology Meeting 2Sangamo BioSciences Announces Presentation of New Data from ZFP Therapeutic® Program in Hemophilia B at American Society for Hematology Meeting 3Sangamo BioSciences Announces Presentation of New Data from ZFP Therapeutic® Program in Hemophilia B at American Society for Hematology Meeting 4The Pharmabiotic Research Institute (PRI) is Preparing to Publish its First Works on the Therapeutic and Medicinal Potential of Probiotics 2The Pharmabiotic Research Institute (PRI) is Preparing to Publish its First Works on the Therapeutic and Medicinal Potential of Probiotics 3The Pharmabiotic Research Institute (PRI) is Preparing to Publish its First Works on the Therapeutic and Medicinal Potential of Probiotics 4
(Date:1/22/2015)... 15, 2015  BellBrook Labs, a leader in high ... launch of a TR-FRET (time resolved Forster resonance ... Assay, a high throughput screening assay for glycosyltransferases ... allow for sensitive detection of hundreds of human ...
(Date:1/22/2015)... , Jan. 20, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... market, reports on the recent success of the Wocket™ smart wallet at ... Wocket smart wallet was named as one of the "11 Hot Products ... Best Products Launched At CES So Far" by Newseveryday.com and "The top ...
(Date:1/22/2015)... Issued by Small Cap IR. In 2011, Nilson Report,s annual ... merchants on the leading payment cards rose to $135.3 billion ... prepaid cards reached 6.54 billion.  Eight million credit card users ... 2012, and credit and debit card fraud resulted in $11.27 ...
Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 2
... Finding cures for hearing loss, breast cancer and childhood ... for tuberculosis are goals of the first recipients of ... Collaborative Research Fund. The fund, a $3 million initiative ... supports collaboration among researchers at Rice University, Texas Children,s ...
... three undergraduates set off on an expedition in 1965 to ... realise that they were establishing the groundwork for a study ... led by the University of York has repeated the survey ... moved uphill by about 67 metres over the intervening years ...
... COLUMBIA, Mo. When it is head versus heart, ... organ to develop and is critical in supplying blood ... known about the complex processes that regulate the heartbeat. ... has identified certain proteins within the heart muscle that ...
Cached Biology News:Texas Medical Center researchers win collaborative grants 2Texas Medical Center researchers win collaborative grants 3Texas Medical Center researchers win collaborative grants 4The global impact of climate change on biodiversity 2Researchers examine developing hearts in chickens to find solutions for human heart abnormalities 2
Human Norrin Biotinylated Affinity Purified PAb Protein Family: Other Growth Factors, Other Wnt-related Molecules...
...
Recombinant Mouse Matrilin-4, CF Protein Family: Extracellular Matrix Molecules, Chondrogenesis Markers...
Request Info...
Biology Products: