Navigation Links
A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

Michael Hepburn and Glenn A. Miller, Ph.D., Molecular Profiling Laboratory, Genzyme, Framingham, MA


Introduction
The search for sequence variations in genomic DNA is important in the study of genes that play a role in the development of cancer and a variety of other single and multiple gene disorders. A wide variety of different methods to detect DNA sequence variations have been developed (Dianzani, 1993). One of these methods, denaturing gradient gel electrophoresis (DGGE), has been shown to be a sensitive, reproducible, reliable technology for use in many research environments (Myers, 1985a; 1985b; 1987).

DGGE involves electrophoresis of double stranded DNA fragments through a polyacrylamide gel containing a linear gradient of denaturant. As the fragment migrates through the denaturant gradient the strands begin to melt. The melting profile of a given DNA duplex is predominantly determined by its base sequence with greater GC content resulting in higher melting temperatures. The ability of DGGE to detect sequence alterations is based on the differential melting characteristics of homoduplex DNA versus heteroduplex DNA. As heteroduplex DNA migrates through the denaturant gradient the areas of non-homology melt at a lower temperature than the comparable homoduplex region. This results in an area of decreased mobility within the fragment, retarding its progress through the gel. This reduction in mobility results in a separation of homoduplex from heteroduplex fragments thereby identifying a region of sequence alteration.

The melting characteristics of a double stranded DNA fragment can be predicted a priori, allowing one to predetermine
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. QIAGEN Multiplex PCR Handbook
3. QIAGEN Multiplex PCR Kit
4. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
5. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
6. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
7. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
8. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
9. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
Post Your Comments:
(Date:4/24/2015)... Telbec/ - Mirego, leader in mobile strategies and solutions, is ... app extensions for the all new Apple Watch. Made in ... and MediaMiser, these extensions will reinvent the way users receive ... wrist use.  Available today in ... tactile smartwatch that makes for an excellent buddy for business, ...
(Date:4/24/2015)... 2015 Seoul Semiconductor announced the ... applications in the lighting market. In recent ... this technology in emerging markets such as Russia, ... range of applications such as streetlight and area ... , First launched in 2005 the Acrich technology ...
(Date:4/23/2015)... Mount Dora, Florida (PRWEB) April 23, 2015 ... announced that it intends to conduct a groundbreaking ... can begin to be reversed in as little ... researchers—using the science of metabolic and nutritional medicine—will ... conditions that cripple and destroy our aging brain. ...
(Date:4/23/2015)... 2015 Six startups from Russia ... 4-17 as part of the U.S.-Russia Innovation Corridor ... of biotechnology startups to participate in the program ... and the University of Maryland (UMD) signed a ... respective universities in the biomedical industry in 2013. ...
Breaking Biology Technology:Familiprix, OMSignal and MediaMiser apps available today for Apple Watch 2Acrich Technology from Seoul Semiconductor Adopted in Installations Worldwide 2New Study Looks to Reverse Alzheimer’s Disease 2New Study Looks to Reverse Alzheimer’s Disease 3U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 2U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 3U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 4
... Mass., March 14, 2011 Advanced Cell Technology, Inc. ... leader in the field of regenerative medicine, announced today ... executive officer, will be presenting at the BIO-Europe Spring ... in Milan, Italy.  Mr. Rabin,s presentation is scheduled for ...
... new measurement technology under a research project funded by ... National Science Foundation is probing the structure of composite ... of the first microscopic insights into a sixty year ... repeated shear strains," said Dr. Daniel Blair, Assistant Professor, ...
... Co., Inc. (NYSE Amex: TPI ), a ... traditional Chinese medicine, branded generics and other pharmaceuticals is ... Growth Stock Conference from March 13 - 16, 2011 ... Dr. James Jiayuan Tong, Chief Financial Officer, Chief ...
Cached Biology Technology:ACT Interim Chairman and CEO Gary Rabin to Present at BIO-Europe Spring 2011 Conference 2New measurement into biological polymer networks 2TPI Attends 23rd ROTH Annual OC Growth Stock Conference from March 13-16, 2011 2
(Date:3/31/2015)... 2015   Guidepoint , a leading global research ... Pain Management TRACKER, part of the Guidepoint TRACKER ... the medical device and therapeutics markets. The Post-Surgical Pain ... volumes, market share, and adoption rates across three major ... surgery. The Post-Surgical Pain Management TRACKER will ...
(Date:3/26/2015)... The Granite Club, Canada,s ... announced it has implemented a state-of-the-art access and security ... and staff, while restricting access to authorized users only. ... IMID Access system because it has the perfect combination ... in addition to unparalleled security," said James DiRenzo ...
(Date:3/24/2015)... Mar. 24, 2015 Research and Markets ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ... Biometrics - Global Strategic Business Report" report to their offering. ... Voice Biometrics in US$ Thousands by the following Segments: Face Biometrics, ... US, Canada , Japan , ... Middle East & Africa , ...
Breaking Biology News(10 mins):Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6
... and Technology (NIST) and the University of Massachusetts Amherst ... are able to accumulate within plants and damage their ... NIST chemist Bryant C. Nelson showed that under laboratory ... plant root cells and generate many mutagenic DNA base ...
... 18, 2012) Autologous (self-donated) mononuclear cells derived from ... vascular growth when transplanted into patients with diabetes who ... artery disease (PAD), a complication of diabetes. The team ... study published their results in a recent issue of ...
... a commitment to the Broad Institute of Harvard and ... that will support some of the most promising and ... The Merkin Family Foundation,s commitment will be used to ... Institute and will provide sustained support for these outstanding ...
Cached Biology News:NIST/UMass study finds evidence nanoparticles may increase plant DNA damage 2Autologous bone marrow-derived mononuclear cell transplants can reduce diabetic amputations 2Merkin Family Foundation to fund next generation of Broad Institute scientists 2
b6-2A12...
... are prepared with pure chemicals according to ... Kit is designed to isolating intact and ... way. One kit is consisted of reagents ... from 10 20 g tissue or ...
... new Enzyme-Linked Immunosorbent Assay (ELISA) analyzer offering ... a dedicated EVO platform for reliableELISA Processing ... incubation units operated by the new sophisticated ... in three sizes - 100, 150, 200 ...
... polypeptide hormones which belong to the ... TGF- superfamily is a large group ... many aspects of development, reproductive function ... incude TGF-s, bone morphogenetic proteins, growth ...
Biology Products: