Navigation Links
A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT ,,, 8100 HTS System

uated independently of the amount of murine IgG secreted into the media. Indeed, reliable data can be obtained for wells containing as high as 22 g/mL IgG to as low as 0.16 g/mL IgG.

Conclusion

The hybridoma screening assay developed for the FMAT system was simple to develop, set-up, and execute. Cells for the fluorometric microvolume assay were plated just minutes prior to the screen, unlike the cell ELISA where cells had to be plated 48 hours prior to the screen. This eliminates any edge effects due to uneven cell growth across the plate and reduces cell culture burden. Because of the mix-and-read format of the assay, all cell types (suspension or adherent) can be used without further manipulations. With a cell ELISA, suspension or weakly adherent cells must be fixed by some means to the well surface and thereby introduces potentially detrimental variables to the screen. Besides cell-surface antigens, the FMAT system can be used to screen for monoclonal antibodies directed against soluble antigens by using polystyrene beads coated against the antigen (Figure 5). With both the cell- and bead-based assays, no wash steps and only one incubation step are required. Moreover, only 5 L of the hybridoma supernatant is required for the assay, enabling parallel multi-specificity screening at the fusion plate stage. In contrast, the cell ELISA typically requires 50 to 100 L of hybridoma supernatant. In addition, the FMAT system allows for multiplexed assays based on bead size or dye color, enabling more than one assay to be performed in a single well. With its ease-of-use, flexibility and sensitivity, the FMAT system is ideally suited for hybridoma screening.
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
4. Organize Your Data from the Big Blue Mutation Assay
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
7. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Using Validated siRNAs in Functional Genomic Assays
10. Nuclease Protection Assays: The Basics
11. RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay
Post Your Comments:
(Date:10/22/2014)... -- Research and Markets  has announced the addition ... Application, Geography - Global Analysis and Forecast (2014 - ... Organic electronics, also called as polymer electronics or plastic ... with small conductive molecules and electrically conductive polymers. It ... are carbon based, made using synthetic strategies developed in ...
(Date:10/20/2014)... Convey Computer , the leader ... Iowa State University won first place in the ... HC-2ex, the team’s solution achieved the highest overall performance ... second place finisher. , Experts from all segments of ... long challenge, using a variety of design tools, hardware ...
(Date:10/20/2014)... -- GenVec, Inc. (NASDAQ: GNVC ) today announced the ... board of directors effective on October 24, 2014.   Dr. Horovitz ... its chairman from June 2006 to November 2013.  During his ... and Audit Committees of the board.  "We ... service to GenVec, and its stockholders," said Wayne T. ...
(Date:10/20/2014)... 20, 2014 Local veterinary surgeon, Dr. ... investigational study of donor stem cells for dogs with ... therapy and has performed clinical stem cell therapy for ... to determine if a single injection of donor stem ... help reduce pain and inflammation in the treated joints. ...
Breaking Biology Technology:Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 2Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 3Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 2Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 3Zola P. Horovitz To Retire From GenVec Board 2Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2
... INDIANAPOLIS, Feb. 8 Semafore Pharmaceuticals Inc.,today announced ... and Ullrich Schwertschlag, M.D., Ph.D., chief medical officer,will ... programs at,2:45 p.m. EST on Wednesday, Feb. 13, ... New York City., The update will include ...
... onTargetjobs, the leading,niche Internet job ... of,RegionalHelpWanted.com, a network of over 350 ... is the parent company of BioSpace.com, ... career services to the biotechnology, pharmaceutical,hospitality, ...
... Remain as Advisor to Company, FRAZER, Pa., Feb. ... Executive Vice President, General Counsel &,Secretary, John E. Osborn, ... March 31. Mr. Osborn has agreed to remain as ... matters., "John has been an invaluable member of ...
Cached Biology Technology:Semafore to Present at BIO CEO & Investor Conference 2onTargetjobs Announces Acquisition of RegionalHelpWanted.com 2Cephalon General Counsel John E. Osborn to Resign Position 2Cephalon General Counsel John E. Osborn to Resign Position 3
(Date:10/15/2014)... Gutenberg University Mainz (JGU) as a full member, ... maintain a significant nationwide standing in the field ... Johannes Gutenberg University Mainz as a full member ... national and international competition for the best minds ... be proud that Mainz University has been recognized ...
(Date:10/15/2014)... linked increased resistance to bacterial pneumonia in female mice ... estrogen. , Females are naturally more resistant to respiratory ... has shown that increased resistance to bacterial pneumonia in ... synthase 3 (NOS3). They also show that this enzyme ... sex hormone estrogen. , The team, lead by Professor ...
(Date:10/15/2014)... Geneticist Fred Alt will be awarded the 44th Rosenstiel ... University for his pioneering research exploring the mechanisms of ... and cancer cells. Alt is the second alumnus to ... the Nobel Prize in 2003. , Alt is the ... Genetics at Harvard Medical School and an investigator at ...
Breaking Biology News(10 mins):Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 3Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2
... bonds that tie a mother to her newborn may be ... deliver by cesarean section, according to a study published by ... Journal of Child Psychology and Psychiatry . ... James Swain, M.D., recruited two groups of parents from postpartum ...
... This press release is available in German . ... checks above all at airports has been increased ... protection of passengers. Possibilities for new and safe methods of ... can be used for this purpose, however, it has to ...
... into the mating and nesting practices of a common ... males sequentially the highest recorded of any vertebrate. ... Biological Sciences, has researched the frog species Bibron,s toadlet ... field trip, discovered a new behaviour undetected in a ...
Cached Biology News:Natural childbirth linked to stronger baby bonding than C-sections 2Checking people at airports -- with terahertz radiation 2Australian frog species chooses not to put eggs in 1 basket 2
A Highly Purified, Cost-Effective Taq DNA Polymerase...
... , Ellman's reagent *M: ... *Storage: RT, When an enzyme-catalyzed reaction ... present in the substrate, it may be possible ... with the product to form a chromophore. The ...
Cag-A (IgG) -Ab EIA Sample Size: 10 l...
... a recombinant human protein produced in E. coli ... RNase A type enzymes. The mode of ... in a 1:1 ratio. The enzyme has been ... and cell types. Addition of the ribonuclease inhibitor ...
Biology Products: