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A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)

determination of ID50 values using limited amounts of reagents.


REFERENCES
1. Bush, K., Jacoby, G. A. and Medeiros, A. A. A functional classification scheme for β -lactamases and its correlation with molecular structure. Antimicrobial Agents and Chemotherapy 39:1211-1233 (1995).

2. Holt, J., Simpson, I.N. and Harper, P.B. Quantitative methods for assessing the stability of β -lactam antibiotics to cell-free b -lactamase extracts. Antibiotics: Assessment of Antimicrobial Activity and Resistance (Russell, A.D. and Quesnel, L.B., Eds.) pages 127-139 (1983).

3. Khushi, T., Payne, D.J., Fosberry, A. and Reading. Production of metal dependent β -lactamases by clinical strains of B.fragilis isolated before 1987. Journal of Antimicrobial Chemotherapy 37:345-350 (1996).

4. Neu, H.C., Wilson, A.P.R., Gruneberg, R.N. Amoxycillin/clavulanic acid: a review of its efficacy in over 38,500 patients from 1979-1992. Journal of Chemotherapy 5:67-93 (1993).

5. Payne, D.J., Cramp, R., Winstanley, D.J. and Knowles, D.J.C. Comparative activities of clavulanic acid, sublactam and tazobactam against clinically important β -lactamases. Antimicrobial Agents and Chemotherapy 38, 767-772 (1994).


ACKNOWLEDGMENTS
We thank Dr. David Livermore of the Department of Medical Microbiology, London Hospital Medical College, for the bacterial strain producing SME-1.


AUTHORS
Dr. David J. Payne and Sarbendra Pradhananga
Department of Molecular Microbiology, SmithKline Beecham Pharmaceuticals, 1250 S. Collegeville Rd., PO Box 5089, Collegeville PA 19426-0989, USA


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