Navigation Links
A DELFIA Assay for Inhibition of PARP

may actually be a suicide response, since it causes rapid NAD and ATP depletion and leads to cell death before the cell can repair the DNA damage.

METHODS

The DNA used in the polymerization reaction was fragmented by hydrodynamic shearing. The DNA was passed through the orifice of a 28-gauge hypodermic needle. This method of shearing generates 1.5-2.0 kb DNA fragments5. The polymerization reaction was performed in an uncoated 96-well microtiter plate. To the well was added: 2.5 μg histones (Trevigen/Upstate U.C. Biotechnology), 1 μg DNA (Sigma), 15 μM NAD + Bio-NAD (Trevigen) in a 17:1 or 1:1 ratio, 1 mM 3- aminobenzamide6 (inhibitor of PARP, Sigma) 0.5 U PARP enzyme (Trevigen) and PARP buffer (500 mM Tris-HCl, 250 mM MgCl2). The reaction was performed at room temperature, for 30 minutes. The plate was then washed 2 times using the DELFIA Platewash. Eu-labelled streptavidin was diluted in DELFIA Assay Buffer to 200 ng/ well and incubated for 30 minutes at room temperature. The plate was washed 4 times using the DELFIA Platewash, prior to addition of 200 μL/well DELFIA Enhancment Solution. The fluorescence was measured using VICTOR2 TM plate reader.

The data shows that the DELFIA timeresolved fluorescence assay for inhibition of PARP allows for the measurement of the incorporation of biotinylated NAD. This technology is therefore suitable for the screening of inhibitors of PARP where the formation of poly(ADP-ribose) chains is inhibited.

REFERENCES:

1. Satoh, M. S. and Lindahl, T. 1994. Role of poly(ADP-ribose) formation in DNA repair. Nature 356, 356-358

2. Lautier, D., Langeux, J., Thibodeau, L., Menard, L. and Poirier, G. G. 1993. Molecular and biochemical features of poly(ADP-ribose) metabolism. Mol Cell Biochem '"/>

Source:


Page: All 1 2 3

Related biology technology :

1. DELFIA Protein Kinase assays.
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
4. Organize Your Data from the Big Blue Mutation Assay
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
7. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Using Validated siRNAs in Functional Genomic Assays
10. Nuclease Protection Assays: The Basics
11. RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: DELFIA Assay for Inhibition PARP

(Date:5/21/2015)... SQI Diagnostics Inc. ("SQI" or the "Company") (TSX-V: SQD; ... proprietary technologies and products for advanced microarray diagnostics, today ... fiscal quarter ended March 31, 2015. ... with our existing customers and to develop new opportunities," ... SQI. "The delivery of a fully automated sqidlite™ system ...
(Date:5/21/2015)... May 21, 2015 uBiome, the ... a partnership with PicnicHealth, a healthcare company that ... diagnosed with Inflammatory Bowel Disease (IBD) will receive ... complementary uBiome research kit. Both companies were funded ... , For more information on this partnership ...
(Date:5/21/2015)... , May 21, 2015  CytRx Corporation ... development company specializing in oncology, today announced positive ... trial with aldoxorubicin for the treatment of unresectable ... cancer.  The open-label, multisite trial is designed to ... in patients whose tumors have progressed following prior ...
(Date:5/21/2015)... PA (PRWEB) May 21, 2015 ... has joined the firm as senior statistician and Principal, ... years of experience in the management and analysis of ... pharmaceutical and chemical industries. , “We’re delighted to have ... capabilities in data analytics,” said Dr. Philippe Cini, Group ...
Breaking Biology Technology:SQI Diagnostics Reports Second Quarter Financial Results 2SQI Diagnostics Reports Second Quarter Financial Results 3SQI Diagnostics Reports Second Quarter Financial Results 4SQI Diagnostics Reports Second Quarter Financial Results 5SQI Diagnostics Reports Second Quarter Financial Results 6SQI Diagnostics Reports Second Quarter Financial Results 7SQI Diagnostics Reports Second Quarter Financial Results 8uBiome Partners with PicnicHealth 2uBiome Partners with PicnicHealth 3CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 2CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 3CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 4CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 5CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 6CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 7Julia O’Neill Joins Tunnell Consulting’s Operational and Process Improvement Practice 2
... student teams with high-tech diagnostic and surgical devices ... annual BMEidea awards at MD&M East Conference in ... June 10 Today three collegiate biomedical engineering ... The winners in the annual Biomedical Engineering Innovation, ...
... ... gene synthesis has long been misunderstood. DNA2.0 has cracked the code on ... technology. DNA2.0,s approach goes beyond codon arrangement, so the company is introducing ... patent has been proven to increase protein expression yields by up to ...
... Ongoing , , DALLAS, June 10 ... (OTC Bulletin Board: ACCP) , announced today ... Patent and Trademark Office that two US patents relating to ... the management of mucositis will issue in June as US Patent ...
Cached Biology Technology:NCIIA Honors Top Collegiate Biomedical Engineering Teams 2NCIIA Honors Top Collegiate Biomedical Engineering Teams 3DNA2.0 Awarded US Patents for Breakthrough Gene Optimization Technology -- Company's Unique Algorithm Enables Expression Yields up to 50 Times Greater than Competing Approaches 2DNA2.0 Awarded US Patents for Breakthrough Gene Optimization Technology -- Company's Unique Algorithm Enables Expression Yields up to 50 Times Greater than Competing Approaches 3Access Pharmaceuticals Announces Granting of Two US Patents for MuGard(TM) 2Access Pharmaceuticals Announces Granting of Two US Patents for MuGard(TM) 3
(Date:5/8/2015)... JOSE, Calif. , May 8, 2015 Synaptics ... interface solutions, today announced that members of the executive management ... J.P. Morgan 43rd Annual Technology, Media and Telecom Conference ... Waterfront Hotel in Boston, MA ... May 27, 2015 Time: 2:45pm ET Location: The New York ...
(Date:4/27/2015)... , April 27, 2015  For more than ... trade show has been the premier worldwide event for ... 2015 is a must-attend event for any local, national, ... into the emerging commercial markets and current applications of ... innovations of this projected $48 billion industry, and how ...
(Date:4/20/2015)... PASADENA, Calif. , April 20, 2015 ... San Gabriel Valley to implant a new miniaturized, wireless ... HF System is the first and only FDA-approved heart ... reduce hospital admissions when used by physicians to manage ... a sensor that is implanted in the pulmonary artery ...
Breaking Biology News(10 mins):Synaptics to Present at Upcoming Investor Conferences 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3
... School in Plymouth, UK, have carried out research that suggests ... children from health experts may not be enough to tackle ... been published in the most recent issue of the journal ... the EarlyBird study, which has followed the development of over ...
... Austrian and Swiss scientists conducted intensive studies of the ... mountains and valleys. Around the same time, the Swiss ... at locations above 5000 metres, studying and photographing the ... black and white photographs taken by these scientists are ...
... be the focus of the Society for Nutrition Education,s ... one small step in fighting world hunger. Food security ... immediate attention in order to combat the issues of ... Nutrition Education (SNE) encourages its members and the public ...
Cached Biology News:Recommendations for children's exercise lacking say experts 2'Himalaya -- Changing Landscapes' photo exhibition draws attention to the impacts of climate change 2'Himalaya -- Changing Landscapes' photo exhibition draws attention to the impacts of climate change 3World Food Day brings attention to food security around the globe 2
tracking dye in electrophoresis; free acid, C 19 H 10 BrO 5 S; MW: 669.96...
... Feature Extraction Software reads ... files to prepare microarray data ... * one 1-year license ... commercial accounts , * ...
... Radiochemical Purity is >95%NEN Radiolabeled Ligands\n\nReceptor-related ... continual availability of new radiolabeled ligands selected ... offers a wide range of products and ... including over 400 state-of-the-art radioligands. If you ...
... system provides excellent performance with isocratic ... suppression, and column heater all standard, ... sensitivity and stability. The vacuum degas ... trace-level work. Operate in manual or ...
Biology Products: