Navigation Links
A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7,,,Cell Lines

line, the more transfection is achieved. To maximize the amount of lipid, we compared the concentration of cell proteins after transfections (data not shown). The A549 cell line seemed extremely tolerant to LipoTAXI transfection reagent as determined by the measurement of the total protein for the various volumes used. Even for 20 l of LipoTAXI transfection reagent, the concentration of cell protein was constant. For the other transfection agents, toxicity to cells increased as their amounts increased. Similar results were found when COS-7 and NIH 3T3 cell lines were transfected using 12 and 18 l of LipoTAXI transfection reagent, respectively. As shown in Figure 2, for the three volumes used, the other reagents demonstrated lower transfection efficiencies in all three cell lines.

Conclusions

These transfection studies first optimize LipoTAXI transfection reagent and then compare its efficacy to four other popularly used transfection reagents. Our comparison shows the superior efficiency and low toxicity of LipoTAXI reagent, features that make it the best transfection reagent for transfections in A549, COS-7 and NIH 3T3 cells.

REFERENCES
  1. Ausubel, F.M., et al. (1994) In Current Protocols in Molecular Biology. Wiley & Sons, New York.

  2. Jordan, M., Schallhorn, A., and Wurn, F.M. (1996) Nucleic Acids Res. 24: 596-601.

  3. Pingerelli, P., Petre, M., and Karmiol, S. (1997) Strategies 10: 8-9.

  4. Eustice, D.C., et al. (1991) Biotechniques 11: 739-743.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
2. Performance Comparisons of Commercial RT-PCR Systems
3. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
4. TripleMaster PCR System: Direct Comparison with Competitors
5. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
6. Comparison of different protein quantitation methods
7. Comparison of Different Methods of mRNA Quantitation
8. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
9. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
10. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
11. Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Comparison Study Lipid Tranfection Reagents NIH and COS Cell Lines

(Date:8/31/2015)... LabStyle Innovations Corp. (OTCQB: DRIO), developer ... it recently closed a round of funding totaling ... support the global rollout and initial U.S. commercial ... management solution that includes novel software applications combined ... device, and for working capital purposes. The Dario ...
(Date:8/31/2015)... Redwood City, CA (PRWEB) , ... August 31, 2015 , ... ... Ablation System. Minerva is the first commercially available new FDA approved system ... high treatment Success rate of 92%, the high Amenorrhea (zero bleeding) rate of 66%, ...
(Date:8/28/2015)... 2015 According to a new ... Storage Systems, Thawing Equipment, Alarms), by Biospecimen (Human Tissue, ... End User - Global Forecast to 2020", published by ... USD 3,731.03 Million by 2020 from USD 2,150.48 Million ... 2015 and 2020. Browse 74 Tables ...
(Date:8/28/2015)... ... August 28, 2015 , ... ... Wade McKenna, DO, announced today that the use of sterile, dehydrated amniotic tissue ... healing of an otherwise non-healing surgical knee wound. , The case involved a ...
Breaking Biology Technology:LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 2LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 3LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 4U.S. FDA Approves Minerva Endometrial Ablation System 2U.S. FDA Approves Minerva Endometrial Ablation System 3Biopreservation Market Worth 3,731.03 Million USD by 2020 2Biopreservation Market Worth 3,731.03 Million USD by 2020 3Biopreservation Market Worth 3,731.03 Million USD by 2020 4Biopreservation Market Worth 3,731.03 Million USD by 2020 5Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 2Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 3Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 4
... Royalty LP 2 (DRC) announced,today the purchase of ... in PEG-INTRON for $92.5M. DRC will also provide ... recognition levels be met.,PEG-INTRON (peginterferon alpha-2b), is approved ... worldwide by Schering-Plough Corporation (SGP). Speaking on ...
... YORK, Aug. 27 The American Stock Exchange(R),(Amex(R)) ... under the ticker,symbol CUR. Neuralstem, Inc. is ... neural stem cell technology in the field of,regenerative ... American Stock Exchange," said,John McGonegal, Senior Vice President ...
... LANSING, Mich., Aug. 27 Neogen Corporation,(Nasdaq: ... leading manufacturer,and marketer of animal health products. ... products through a,network of more than 200 distributors ... products of exceptional quality, including its,well-regarded AG-TEK(R) veterinary ...
Cached Biology Technology:Drug Royalty Corporation (DRC) Announces Acquisition Regarding PEG-INTRON(R) From Enzon Pharmaceuticals, Inc. (ENZN) 2Neogen Acquires Kane Enterprises 2
(Date:8/12/2015)... , Aug. 12, 2015  Synaptics Inc. ... human interface solutions, today announced that four of ... Match-in-Sensor solution, have officially been named FIDO Certified™ ... part of the certification, Synaptics, Natural ID™ fingerprint ... Universal Authentication Framework (UAF) standard and are interoperable ...
(Date:8/6/2015)... and TELTOW, Germany , ... 2015 Siggraph Conference, SensoMotoric Instruments (SMI) shows ... reality , based on Epson,s Moverio BT-200 ... tracking platform. With this new solution, unprecedented quality and ... hands-free interaction with context-sensitive displays. For the first time, ...
(Date:8/4/2015)... 2015 Research ... the addition of the "Digital Forensics ... Sub-Segment (Computer Forensics, Network Forensics, Mobile Device ... Vertical and Region - Global Forecast to ... By Component (Hardware, Software, and Service), ...
Breaking Biology News(10 mins):Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3Digital Forensics Market - The Market Represents a Compound Annual Growth Rate (CAGR) of 11.3% From 2015 to 2020 2
... Walter Leal, professor of entomology at the University ... a major award from the Entomological Society of ... Biochemistry and Toxicology. Leal, former chair of ... his innovative and creative research involving insect communication. ...
... and metal rods to complex virtual humans made of muscle, ... evolving and researchers at Wake Forest University School of Medicine ... new generation of human body models will represent live humans ... The models will contain detailed representation of the bones and ...
... be smarter than we thought. Faced with a dire shortage ... may be able to cross-breed with certain other coral species ... This finding, released by scientists at the ARC Centre ... the ability of the world,s corals to withstand the rigors ...
Cached Biology News:UC Davis chemical ecologist wins major award 2Wake Forest plays integral role in effort to revolutionize vehicle safety 2Wake Forest plays integral role in effort to revolutionize vehicle safety 3Rare corals breed their way out of trouble 2
... TProfessional Thermocycler combines top performance with exquisite ... high speed and unparalleled temperature uniformity. The ... programming in a spreadsheet or alternatively in ... Lid opens on the push of a ...
Preparation TW--Compatibility: no end cap seals -Hybridization Tube for Hybrigene -Comp Dim: 240 80 mm -Material: borosilicate glass -Mfr Desig: Techne FHB-15...
... is Tecans state-of-the-art software for all your ... scheduling in a single, scalable software package. ... consistent, simplifying the control of all Freedom ... Freedom EVO 100 with one liquid handling ...
... Vesicles Isolation Kit provides all the ... synaptic vesicle fraction. The isolation procedure ... the synaptosomes, and differential centrifugation followed ... kit also contains an antibody specific ...
Biology Products: