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A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7,,,Cell Lines

line, the more transfection is achieved. To maximize the amount of lipid, we compared the concentration of cell proteins after transfections (data not shown). The A549 cell line seemed extremely tolerant to LipoTAXI transfection reagent as determined by the measurement of the total protein for the various volumes used. Even for 20 l of LipoTAXI transfection reagent, the concentration of cell protein was constant. For the other transfection agents, toxicity to cells increased as their amounts increased. Similar results were found when COS-7 and NIH 3T3 cell lines were transfected using 12 and 18 l of LipoTAXI transfection reagent, respectively. As shown in Figure 2, for the three volumes used, the other reagents demonstrated lower transfection efficiencies in all three cell lines.

Conclusions

These transfection studies first optimize LipoTAXI transfection reagent and then compare its efficacy to four other popularly used transfection reagents. Our comparison shows the superior efficiency and low toxicity of LipoTAXI reagent, features that make it the best transfection reagent for transfections in A549, COS-7 and NIH 3T3 cells.

REFERENCES
  1. Ausubel, F.M., et al. (1994) In Current Protocols in Molecular Biology. Wiley & Sons, New York.

  2. Jordan, M., Schallhorn, A., and Wurn, F.M. (1996) Nucleic Acids Res. 24: 596-601.

  3. Pingerelli, P., Petre, M., and Karmiol, S. (1997) Strategies 10: 8-9.

  4. Eustice, D.C., et al. (1991) Biotechniques 11: 739-743.


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