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A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7,,,Cell Lines


LipoTAXI reagent is effective with a wide variety of cells

Leslie S. Arrington
Genetic Applications LLC

LipoTAXI transfection reagent can be used for simple and reproducible delivery of foreign DNA into mammalian cells. In this study, we used varying amounts of LipoTAXI transfection reagent to optimize conditions for transfecting a reporter plasmid in three common cell lines. In addition, the efficacy of LipoTAXI reagent was compared to four other leading transfection reagents. In these studies, LipoTAXI reagent outperformed other transfection reagents and was less toxic to cells.

Researchers can improve the efficiency of introducing foreign DNA into different mammalian cell lines by optimizing transfection reagents. Lipid-mediated transfection reagents surpass other types of transfection reagents because they are easy to use, gentle to cells and provide consistent reproducible results.1,2 However, among cell types, lipid-mediated transfection reagents can differ in transfection efficiency and proper dosage. Therefore, the first step to achieving high transfection efficiencies is to optimize the combination of lipid and DNA for each cell line.3 In this study, we examine the transfection efficiency of optimized LipoTAXI transfection reagent in A549, COS-7 and NIH 3T3 cell lines and compare this reagent to four other popular transfection products.

Optimization

For the initial optimization step, the signal strength of a reporter gene is measured as the amount of lipid is varied. The reporter plasmid (1.0 g) encoding the b-galactosidase gene was transfected into COS-7 cells with volumes of LipoTAXI transfection reagent that ranged between 6 and 15 l. The data demonstrate (Figure 1'"/>

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