Navigation Links
A Better Way to Isolate RNA AND Protein From the Same Sample

ed siRNA sequence. (B) 5 x 105 Schneider's Drosophila cells were treated for 48 hours in a 6 well plate with 10 nM of the indicated dsRNA prepared with Ambion's MEGAscript RNAi Kit. Total RNA and protein were isolated with the PARIS Kit and analyzed by Northern blot and Western blot.

Figure 5. Efficient Cellular Fractionation. (A) RNA and protein were isolated from 106 HeLa cells using the PARIS Kit. To allow direct comparison between RNA isolated from different cellular compartments, the same volume of each fraction (~5%) was analyzed by denaturing agarose gel electrophoresis, Northern blot, or Western blot. (B) 3 x 105 HeLa cells were transfected in duplicate in 6 well plates with 1 g of luciferase mRNA and the relative luciferase activity in each protein fraction was measure 18 hours after transfection. Luciferase mRNA was prepared with Ambion's mMESSAGE mMACHINE and Poly(A) Tailing Kits.


back to top


Ordering Information Cat# Product Name Size 1921 PARIS Kit 50 purifications
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Better RNA Amplification
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
5. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. A New C-Terminal GST Vector for Protein Production in S. pombe
10. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better Way Isolate RNA AND Protein From the Same Sample

(Date:1/14/2014)... 2014 EquitiesIQ, a leading informational research ... Alliqua is an emerging biomedical company acquiring, developing, manufacturing, ... market. , Free report download: http://equitiesiq.com/reports/alliqua/ , ... management team and Board, which launched the company’s new ...
(Date:1/14/2014)... Boston, MA (PRWEB) January 14, 2014 iLab ... Michelle Detwiler as the new Director of Product Strategy. In ... as well as iLab sub-teams to guide in the development ... that iLab provides the maximum possible benefit to the scientific ...
(Date:1/14/2014)... Global Record Systems, LLC, (GRS), ... solutions for patients, physicians, the biopharmaceutical industry, regulators, ... the signing of a three-year Research Collaboration Agreement ... (FDA). This initiative is designed to generate ...
(Date:1/14/2014)... January 14, 2014 Kerr Corporation, a leading ... and additional how-to information about dual arch impressions on its ... Dual Arch Impressions,” the blog entry serves up a list ... houses a step-by-step demonstration by Dr. David Little as he ...
Breaking Biology Technology:EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2
... 9 The results of a new study on immune-based ... sites around the country, will be published in the December ... MD, MS, director of the Butler Hospital Memory and Aging ... Warren Alpert Medical School of Brown University, says that this ...
... , , QUEBEC CITY, Dec. 9 /PRNewswire-FirstCall/ ... AEZ) (the "Company"), a global biopharmaceutical company focused on ... appointment of Pierre Lapalme to its Board of Directors. ... has over the course of his career held numerous ...
... , OXFORD, England, December ... Recent Recruitment of Senior Antibody Industry,Leaders and Highlights OBT,s Growth ... Oxford BioTherapeutics (OBT) has today announced the appointment of ... experience to OBT at an exciting phase in the Company,s,development, ...
Cached Biology Technology:Butler Hospital Neurologist Lead Author of Ground Breaking Bio-Engineered Treatment Study for Alzheimer's Disease 2AEterna Zentaris Announces Appointment of New Board Member 2Oxford BioTherapeutics Appoints Michael Moore as Chairman 2Oxford BioTherapeutics Appoints Michael Moore as Chairman 3Oxford BioTherapeutics Appoints Michael Moore as Chairman 4
(Date:7/9/2014)... one of the amphibians with the highest distribution in ... of water where it comes into contact with the ... carried out by the Spaniard Germn Orizaola from the ... these frogs have developed a defensive response to the ... bodies if they co-exist with the crayfish. , Numerous ...
(Date:7/9/2014)... function and increase the survival rate of naturally ... School of Pharmaceutical Sciences, Southwest University, China performed ... administered with low dose of levothyroxine for 3 ... exhibited an obvious improvement in cognitive and an ... The underlying mechanism was demonstrated that levothyroxine treatment ...
(Date:7/9/2014)... vitamin D in their blood are more likely to survive ... highest levels of vitamin D have half the risk of ... reveal. , The study is the first to correlate total ... their diagnosis which includes that produced after exposure to ... long term survival prospects. , The University of Edinburgh ...
Breaking Biology News(10 mins):Frogs have developed rapid defences against the red swamp crayfish 2Frogs have developed rapid defences against the red swamp crayfish 3
... is helping to reduce the drug cravings of addicted rats. ... some parts of the brain lose glutamate receptors from the ... neurons. The researchers have sidestepped this problem by crafting a ... glutamate receptor and, once inside a neuron, serves as a ...
... discovered in tiny snow fleas by Queen's University researchers ... transplantation. , Drs. Laurie Graham and Peter Davies, from ... produced by the fleas to protect themselves against freezing ... Celsius degrees. This would allow organs to be stored ...
... zebrafish may be able to play a critical role in ... that can lead to it - and identified one gene ... , The findings were published in a professional journal, Proceedings ... State University and two Boston hospitals, the Brigham and Women's ...
Cached Biology News:Grabbing addiction by the tail 2Grabbing addiction by the tail 3New antifreeze protein found in fleas may allow longer storage of transplant organs 2Zebrafish may hold key to improved cancer research 2Zebrafish may hold key to improved cancer research 3
Anti-CD74 Monoclonal Antibody Description: 200 l Research Focus: cell surface antigens Storage: 4C Shipping Temperature: 4C...
POU domain, class 2, transcription factor 2,...
HSPBP1 Antibody...
golgi reassembly stacking protein 2, 55kDa...
Biology Products: