Navigation Links
A Better Way to Isolate RNA AND Protein From the Same Sample

he same experimental sample (Figure 1).

Figure 1. Schematic of PARIS Procedure.


Using PARIS, RNA and protein can be isolated simultaneously from whole cell lysates. Alternatively, RNA and protein can be isolated from separate nuclear and cytoplasmic fractions (see "Application: Nuclear vs. Cytoplasmic Fractionation" at right). Tissue or cultured cells are first homogenized in ice-cold Cell Disruption Buffer to prepare a total cell lysate. Since the homogenization is performed quickly on ice and in the presence of detergent, both protein and RNA can be purified directly from this lysate. For RNA isolation, a part of the total cell lysate is immediately mixed with an equal volume of Lysis/Binding Solution. This solution contains a high concentration of guanidinium thiocyanate, a strong chaotropic denaturant that rapidly inactivates cellular ribonucleases. Total RNA is then purified from the mixture using an RNA binding glass fiber filter. After three rapid washing steps, high quality RNA is eluted in a concentrated form. The entire procedure can be completed in less than 20 minutes. Note: This kit is not recommended for tissues with high levels of ribonucleases, such as pancreas.


Compatible with Most Downstream Applications
The RNA isolated from total, nuclear, or cytoplasmic fractions with the PARIS procedure can be used in a variety of downstream applications, including blot hybridization, in vitro translation, cDNA synthesis, and RT-PCR. A DNase I treatment is recommended for RNA that will be used for RT-PCR experiments, especially if using primers that do not flank introns, or for genes that have processed pseudogenes. Ambion's

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Better RNA Amplification
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
5. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. A New C-Terminal GST Vector for Protein Production in S. pombe
10. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better Way Isolate RNA AND Protein From the Same Sample

(Date:9/2/2015)... ... September 02, 2015 , ... Whitehouse Laboratories is excited ... Pharma Ed Resources upcoming educational seminar and training session on Extractable & Leachable ... conference will provide Updates & Case Studies on the Latest Compliance Implications of ...
(Date:9/2/2015)... ... September 02, 2015 , ... The TS5-Q is designed for ... content, to analysis of athletic performance. Producing high-resolution slow-motion video , the ... to process with the naked eye. , Slow motion sequences are commonly ...
(Date:9/1/2015)... , September 2, 2015 ... auf die Forschung und Entwicklung neuartiger Therapien ... chronisch-entzündliche Darmerkrankungen und Reizdarm konzentriert, erhält vom ... dass sein neues Medikament patentiert wird. Das ... Allergien bei Kindern verhindert und Entzündungen vorbeugt.   ...
(Date:9/1/2015)... The American Brain Tumor Association (ABTA) is now accepting ... program. LOIs are due no later than Wednesday, October ... Discovery Grants are one-year, $50,000 grants that fund high risk/high ... or treatment paradigms for adults and children with brain ... earliest stages, in the hopes that it spurs additional ...
Breaking Biology Technology:Whitehouse Laboratories to Participate in Pharm Ed Resources Extractables, Leachables, & Elemental Impurities Educational Seminar 2Whitehouse Laboratories to Participate in Pharm Ed Resources Extractables, Leachables, & Elemental Impurities Educational Seminar 3Fastec Introduces the Quad-HD TS5-Q High-Speed Camera 2Fastec Introduces the Quad-HD TS5-Q High-Speed Camera 3Das Patent von Swecure zur Verhinderung von Allergien bei Kindern wird in den USA zugelassen 2American Brain Tumor Association Seeks Innovative Concepts For Discovery Research Grants 2
... RNA later Tissue Collection: RNA ... Tissue and Cells. , ... processing or appropriate storage of the tissue or cell , ... followed by immediate disruption in lysis ...
... Isolation , , ... different tissues. A comparison of total RNase activities for 8 different , ... from pancreas to brain (Figure 1), which highlights the level of RNase ... , Figure 1. ...
... RNA interference, the biological mechanism ... (dsRNA) induces gene silencing by , ... revolutionizing the way researchers study gene function. For , ... expression of a particular gene in mammalian cell systems, ...
Cached Biology Technology:Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation 2Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation 3Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation 4RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control 2RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control 3RNAi: A How To for New Users 2RNAi: A How To for New Users 3RNAi: A How To for New Users 4RNAi: A How To for New Users 5RNAi: A How To for New Users 6RNAi: A How To for New Users 7RNAi: A How To for New Users 8RNAi: A How To for New Users 9
(Date:8/31/2015)... BURNABY, Canada , August 31, 2015 ... use of biometrics technology in government digitization projects to drive ... According to a recently published TechSci Research report, " India ... India is projected to grow at ... in the market is anticipated on account of extensive use ...
(Date:8/25/2015)... LAKE CITY and BELLEVUE, Wash. ... global leader in advanced robotic systems, announced today it ... robotic unmanned ground vehicle (UGV), at the National Tactical ... City.  The Guardian S is the ... the culmination of years of research and in-field trials ...
(Date:8/20/2015)... Aug. 20, 2015   Sensory, Inc ., ... the user experience and security of consumer electronics ... announced that its TrulySecure™ is the ... to be FIDO Certified™. The FIDO ... with the FIDO UAF (Universal Authentication Framework) 1.0 ...
Breaking Biology News(10 mins):India Biometrics Market to Grow at Over 35% Through 2020, Says TechSci Research 2India Biometrics Market to Grow at Over 35% Through 2020, Says TechSci Research 3Sarcos to Debut First-of-its-Kind Snake Robot 2Sarcos to Debut First-of-its-Kind Snake Robot 3TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 2TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 3TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 4TrulySecure From Sensory Becomes First Face and Voice Biometrics Technology to be FIDO UAF Certified 5
... 13, 2010) Before Alzheimer,s patients experience memory loss, the ... A new study in mouse models by researchers at ... -- the powerhouses of the cell -- are one of ... in the online Early Edition of PNAS, also found that ...
... Ind. -- The red, metal-laden sludge that escaped a containment ... with the help of carbon sequestration, says an Indiana University ... The bauxite residue now covers 40 square kilometers south ... eight Hungarians and injured at least 150. The residue also ...
... , SALT LAKE CITY, Oct. 13, 2010 - Microbubbles are ... during a demonstration project in eastern China, microbubble technology developed ... a wide range of environmental cleanup efforts around the world. ... organics and heavy metals from industrial sites, and removing harmful ...
Cached Biology News:Early role of mitochondria in AD may help explain limitations to current beta amyloid hypothesis 2Can Hungary's red sludge be made less toxic with carbon? 2University of Utah microbubbles clean dirty soil in China 2University of Utah microbubbles clean dirty soil in China 3
... a direct antibiotic-based selection ... Recombinant selection depends solely ... the need for costly ... and inexpensive process provides ...
These stopping screens are for the biolistic PDS-1000 He and Hepta systems. Supplied as 500 screens....
... Geneious does sequence alignment, 3D structure ... and more; all in a single application. ... several NCBI databases, Uniprot, sort and filter ... how you want it, rapidly searching, filtering ...
... a comprehensive suite of advanced statistics ... to reliably extract biological signals from ... containing thousands of samples, Partek Genomics ... will analyze large data sets on ...
Biology Products: