Navigation Links
A 20

ng so, set the cell concentration to 3.8 x 106 cells/ml.
  • Add and mix plasmid DNA (25 g/ml final concentration, in bidistilled H2O).
  • Transfer 800 l cell suspension into electroporation cuvettes (4 mm gap width). The cell suspension must be free of air bubbles.
  • Electroporation:

    Mode Eukaryotes Voltage (V) 600 V Time constant (T) 50 s No. of pulses (n) 2
  • After the pulse, allow the cell suspension to stand in the cuvette for 5-10 minutes at room temperature.
  • Carefully transfer the cell suspension from the cuvette to 5 ml RPMI 1640 / 0.5% FCS, and centrifuge it for 5-10 minutes, 200 x g, at room temperature. Remove supernatant.
  • Resuspend the cells in 3 ml RPMI 1640 / 10% FCS, and cultivate them in a 55 mm culture dish.
  • Detection methods for transfection:
    The expression of the plasmid pEGFP-N1 can be detected clearly after 24-48 hours with the aid of FACS analysis or under a fluorescence microscope. Result: Survival rate: 250% Transfection rate: 75% based on the initial number of cells used for the experiment Results were measured 24 hours after transfection.


    '"/>

    Source:


    Page: All 1 2

    Related biology technology :

    1. 721.221
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG:

    (Date:8/27/2014)... NEW YORK , Aug. 27, 2014 /PRNewswire/ ... report is available in its catalogue: ... http://www.reportlinker.com/p02316662/Global-Chelating-Agents-Market-2014-2018.html About Chelating Agent ... can form covalent bonds with metal ions, thereby ... effect that some metal-ions have on chemical processes, ...
    (Date:8/27/2014)... 27, 2014  Lisa Kulik saw fireworks for the ... holiday, thanks to a groundbreaking retinal implant co-invented by ... clinician-researcher at the University of Southern California (USC) Eye ... Peoria, Ariz. resident who has retinitis pigmentosa, ... sight. On June 2, she became the first person ...
    (Date:8/27/2014)... (PRWEB) August 27, 2014 “We are ... technology vendors,” said Ven Thangaraj, CEO of Radiant Sage. ... that are taking the imaging corelab market to a ... corelabs and research organizations to efficiently and effectively manage ... the data to be analyzed and read by trained ...
    (Date:8/27/2014)... , Aug. 27, 2014 Reportbuyer.com ... The Graphene Opportunity - A Rational View ... Graphene The New Nanotech? A similar amount ... of applications ranging from microelectronics to water treatment, ... announced the availability of the Graphene Opportunity Report, ...
    Breaking Biology Technology:Global Chelating Agents Market 2014-2018 2Global Chelating Agents Market 2014-2018 3Global Chelating Agents Market 2014-2018 4USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 3USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 4USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 5Radiant Sage Named to CIOReview List of 100 Most Promising Technology Companies 2The Graphene Opportunity - A Rational View 2The Graphene Opportunity - A Rational View 3The Graphene Opportunity - A Rational View 4
    ... current science and experience does not support the safety ... therapies. At a public hearing last week, the Plasma ... and Drug Administration (FDA) to make patient safety its ... evaluation of plasma protein therapies for the biosimilars process. ...
    ... An Agreement in Principle with NOVA Home Loans ... ... if (typeof(lpUnit)==,undefined,) var lpUnit=,PRN,; --> ... s = ,; var first_result; // Results are keyed by longUrl, so we need to grab the first one. ...
    ... study from Thomson Reuters released today shows that the US is ... not kept pace with the increased output from European and Asia-Pacific ... , found that research output from the US has been surpassed ... nearly 40 percent of research papers in the most influential journals(1). ...
    Cached Biology Technology:Plasma Protein Therapies Not Suitable for Biosimilars 2Plasma Protein Therapies Not Suitable for Biosimilars 3Plasma Protein Therapies Not Suitable for Biosimilars 4Dynamic Ventures Corp. Reaches An Agreement in Principle with NOVA Home Loans 2Dynamic Ventures Corp. Reaches An Agreement in Principle with NOVA Home Loans 3Dynamic Ventures Corp. Reaches An Agreement in Principle with NOVA Home Loans 4Dynamic Ventures Corp. Reaches An Agreement in Principle with NOVA Home Loans 5Dynamic Ventures Corp. Reaches An Agreement in Principle with NOVA Home Loans 6Dynamic Ventures Corp. Reaches An Agreement in Principle with NOVA Home Loans 7US Dominance in Scientific Research Significantly Challenged by Increased Competition from Europe and Asia, According to Thomson Reuters Study 2
    (Date:8/27/2014)... mysteries of the human genome was unveiled today in ... detailed comparison yet of how the genomes of the ... research, appearing August 28 in in the journal ... three species, genomes is "read out," and how their ... results add billions of entries to a publicly available ...
    (Date:8/27/2014)... Many African-Americans may not be getting effective doses of ... Hopkins suggests. The initial dosing studies, completed before the ... generally lack a protein that is key to removing ... people with maximum levels of the protein including ... maraviroc in their bodies compared to those who lack ...
    (Date:8/27/2014)... SALT LAKE CITY, Aug. 27, 2014 University of ... in his native Turkey, has won that nation,s highest ... Medal of Science. , Sekercioglu is among five researchers ... Scientific and Technical Research Council of Turkey. Three researchers ... won the Special Award, which is equivalent to ...
    Breaking Biology News(10 mins):Encyclopedia of how genomes function gets much bigger 2Encyclopedia of how genomes function gets much bigger 3Encyclopedia of how genomes function gets much bigger 4Dosage of HIV drug may be ineffective for half of African-Americans 2University of Utah biologist wins Turkey's top science prize 2
    ... 1, 2010) The goal of tissue engineering is ... and then transplant them into patients, where they are fully ... of high-throughput imaging systems that allow researchers to directly monitor ... improving the culturing and implantation techniques, as well as the ...
    ... JOLLA, Calif., April 1, 2010 Though heart disease ... little is understood about its genetic underpinnings. Recently, an ... Biotechnology of the Austrian Academy of Sciences (IMBA), Sanford-Burnham ... light on the subject. Studying Drosophila (fruit flies), the ...
    ... 2010 Imagine being able to image life as it ... begins beating. A professor at the University of Houston, ... doing just that. Kirill Larin, assistant professor ... UH, and his colleagues in the Texas Medical Center are ...
    Cached Biology News:Tissue engineering, imaging neuronal circuits featured in Cold Spring Harbor Protocols 2Mapping heart disease 2The embryonic heart: Imaging life as it happens 2
    ... to MLF1 Interacting Protein/PBIP1 (phospho T78) ... applications). Antigen: ... acids 73-83 of Human MLF1 Interacting ... ID: 359815 ...
    ... space and organize gloves, safety glasses, ... are the perfect benchtop storage bins ... processing and manufacturing. Clear static-dissipative PVC ... they attract. Many other sizes and ...
    ... These handy dispensers save space ... wipers, hats, and other garb. ... storage bins for small parts ... manufacturing. Clear acrylic. Many other ...
    ... Microarrays designed for genome-wide microRNA ... flexible and powerful Paraflo microfluidic on-chip ... as part of our comprehensive microRNA ... Content The Fishes microRNA Microarray ...
    Biology Products: