Navigation Links
96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation

Lynn Jordan1 and Brent Butler2
1Caliper Life Sciences Inc., Applications Laboratory, Hopkinton, Massachusetts, USA
2GlaxoSmithKline, Department of Molecular Screening, Research Triangle Park, North Carolina, USA

Introduction

Initially, work in 96 well microplates was commonly carried out using manual pipetting. As the demand for greater throughput of plate processing has grown, automated liquid handling has become a necessity for most laboratories. The most critical aspect of an automated liquid handler is to understand its performance, typically determined by precision and accuracy.

At Caliper Life Sciences, precision and accuracy testing is achieved by dispensing a tartrazine solution into microplates containing water. This is referred to as a "liquid-to-liquid" dispense. The purpose of this paper is to report the performance obtained by dispensing 0.5 to 2μL aqueous samples containing tartrazine into 96 well plates containing water. Precision and accuracy results are shown for this work.

Materials and Methods

Instrumentation
Testing was performed using a RS-232 RapidPlate 96/384 Workstation (Caliper Life Sciences) using 100μL Automation Certified Pipette TipsTM (Caliper Life Sciences). A Bulk Dispenser (Caliper Life Sciences) was utilized for bulk dispensing across the columns of the microplate (secondary dispensing). Absorbance was measured at 450nm using a Wallac Victor2 Multilabel Counter (PerkinElmer).

Reagents
Tartrazine (Sigma Chemical Company, Catalogue number T-0388) is dissolved in deionized water. Concentrations of 6.0, 1.4, 0.7 and 0.14g/L corresponding to both 1 and 2, 5, 10 and 25μL dispenses respectively are prepared by dissolving the tar
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
3. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
4. Sciclone ALH Bulk Dispense Precision Testing
5. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
6. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
7. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:7/11/2014)... July 11, 2014 /PRNewswire-iReach/ -- Maxim Peptide, a website ... are used solely in scientific experiments, is currently celebrating ... in business, Maxim Peptide, which was created by the ... new and updated website that now features a special ... been updated with fascinating articles about peptide research, including ...
(Date:7/11/2014)... July 11, 2014 BCC Research ( ... DIGITAL POLYMERASE CHAIN REACTION (PCR) TECHNOLOGY: GLOBAL MARKETS , ... technology reached nearly $140 billion in 2013. This is ... a five-year compound annual growth rate (CAGR) of 28.6%. ... of interesting and exciting possibilities within the broader PCR ...
(Date:7/11/2014)... July 11, 2014  Researchers have pioneered a ... The new technology, called Virtual Finger, allows scientists ... like neurons and synapses using the flat surface ... makes 3D imaging studies orders of magnitude more ... unprecedented level across many areas of experimental biology. ...
(Date:7/10/2014)... 10, 2014 Product and process ... low abundance and are often “lost in the ... , Join presenters Dr. Rowel Tobias, Senior Scientist, ... Anders, Head of Quality at Nanotherapeutics, Inc., to ... can speed detection and quantitation while achieving the ...
Breaking Biology Technology:Maxim Peptide Celebrates Second Anniversary Online with an Updated Website and Special Deals Section 2Global Market for Digital Polymerase Chain Reaction Technology to Reach $490 Billion by 2018; Equipment Sector Accounts for the Fastest Growing Segment With 33% CAGR 2Global Market for Digital Polymerase Chain Reaction Technology to Reach $490 Billion by 2018; Equipment Sector Accounts for the Fastest Growing Segment With 33% CAGR 3Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 3Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2
... 14, 2011 Isis Pharmaceuticals, Inc. (Nasdaq: ISIS ... Results and HighlightsWhen: , Monday, February 28, 2011 at 4:30 ... on the Internet.  Simply log onto our Web site listed ... event, a replay of the webcast will be available for ...
... YORK and SAN DIEGO, Feb. 14, 2011 Polaris Group ... announced the enrollment of the first patient in a Phase ... small cell lung cancer (SCLC).  The trial, sponsored by LICR, ... University Medical Center and will soon commence at St. Bartholomew,s ...
... NEW YORK, Feb. 11, 2011 Even with radical ... the Quick Extender Pro penile extender system ... penis enlargement , says QuickExtenderPro.com, the leading provider ... professionals worldwide.   With the emergence of ...
Cached Biology Technology:Polaris Group and The Ludwig Institute for Cancer Research Ltd Initiate Phase 2 Trial of Amino-Acid Degrading Enzyme, ADI-PEG 20, in Patients with Small Cell Lung Cancer 2Polaris Group and The Ludwig Institute for Cancer Research Ltd Initiate Phase 2 Trial of Amino-Acid Degrading Enzyme, ADI-PEG 20, in Patients with Small Cell Lung Cancer 3QuickExtenderPro.com Penis Extender Device Engineered for Quick, Safe and Effective Alternative to Penis Enlargement Surgery 2
(Date:7/10/2014)... Pa. , July 2, 2014  Unisys Corporation,s (NYSE: ... , Unisys Nederland N.V., today announced that it has ... deliver, implement and manage a new Basic Provision Biometrics solution ... Unisys won the contract with a solution based ... management software. The term of the contract is up to ...
(Date:7/10/2014)... , July 3, 2014 ... Market by Type (Swipe And Area), Material (Optical Prism, ... Healthcare, Commercial Security and Banking & Finance), and Geography ... published by MarketsandMarkets, the global Fingerprint Sensors Market is ... a high CAGR of 16.8% from 2014 to 2020. ...
(Date:7/10/2014)... July 7, 2014 According to ... "Electronic Access Control Systems Market Global Forecast, Market Share, ... global Electronic Access Control systems market was valued at ... grow at a CAGR of 12.6% from 2014 to ... million in 2019. Browse the full Electronic ...
Breaking Biology News(10 mins):Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 3Fingerprint Sensors Market worth $14.35 Billion by 2020 2Fingerprint Sensors Market worth $14.35 Billion by 2020 3Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 2Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 3Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 4Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 5
... Solenosteira macrospira . He does all the work of raising ... the baby snails are his own. The surprising new finding ... S. macrospira in a small club of reproductive outliers ... cannibalism, and the species has one of the most extreme life ...
... YORK, August 28, 2012 Researchers at Polytechnic Institute ... biosensor capable of identifying the smallest single virus particles ... detection in a point-of-care setting and shrink test result ... Arnold, university professor of applied physics and member of ...
... the aminopyridine class not only has the potential to become ... but might also be able to block transmission of the ... involving the Medicines for Malaria Venture (MMV), based in Switzerland, ... University of Cape Town, South Africa. On the basis of ...
Cached Biology News:Male snails babysit for other dads 2Male snails babysit for other dads 3By detecting smallest virus, researchers open possibilities for early disease detection 2By detecting smallest virus, researchers open possibilities for early disease detection 3African research identifies strong candidate for possible single-dose malaria cure 2African research identifies strong candidate for possible single-dose malaria cure 3
... Antibody, Unconjugated Applications: ... dilution of 1/100. IP: Use at ... at an assay dependent dilution. Predicted ... in other applications. Optimal dilutions/concentrations ...
Kit containing MACS equipment and reagents for immunopurification of proteins....
Membrane Proteins from over 50 different human Alzheimer, Diabetic, Liver Cirrhosis, and Lupus diseased tissues are available....
... Successful biomarker discovery projects require ... samples to distinguish clinically relevant data ... CiphergenExpress Data Manager is now available ... sample tracking and data flood inherent ...
Biology Products: