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96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation

Lynn Jordan1 and Brent Butler2
1Caliper Life Sciences Inc., Applications Laboratory, Hopkinton, Massachusetts, USA
2GlaxoSmithKline, Department of Molecular Screening, Research Triangle Park, North Carolina, USA

Introduction

Initially, work in 96 well microplates was commonly carried out using manual pipetting. As the demand for greater throughput of plate processing has grown, automated liquid handling has become a necessity for most laboratories. The most critical aspect of an automated liquid handler is to understand its performance, typically determined by precision and accuracy.

At Caliper Life Sciences, precision and accuracy testing is achieved by dispensing a tartrazine solution into microplates containing water. This is referred to as a "liquid-to-liquid" dispense. The purpose of this paper is to report the performance obtained by dispensing 0.5 to 2μL aqueous samples containing tartrazine into 96 well plates containing water. Precision and accuracy results are shown for this work.

Materials and Methods

Instrumentation
 Testing was performed using a RS-232 RapidPlate 96/384 Workstation (Caliper Life Sciences) using 100μL Automation Certified Pipette TipsTM (Caliper Life Sciences). A Bulk Dispenser (Caliper Life Sciences) was utilized for bulk dispensing across the columns of the microplate (secondary dispensing). Absorbance was measured at 450nm using a Wallac Victor2 Multilabel Counter (PerkinElmer).

Reagents
Tartrazine (Sigma Chemical Company, Catalogue number T-0388) is dissolved in deionized water. Concentrations of 6.0, 1.4, 0.7 and 0.14g/L corresponding to both 1 and 2, 5, 10 and 25μL dispenses respectively are prepared by dissolving the tar
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