Navigation Links
96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation

Lynn Jordan1 and Brent Butler2
1Caliper Life Sciences Inc., Applications Laboratory, Hopkinton, Massachusetts, USA
2GlaxoSmithKline, Department of Molecular Screening, Research Triangle Park, North Carolina, USA

Introduction

Initially, work in 96 well microplates was commonly carried out using manual pipetting. As the demand for greater throughput of plate processing has grown, automated liquid handling has become a necessity for most laboratories. The most critical aspect of an automated liquid handler is to understand its performance, typically determined by precision and accuracy.

At Caliper Life Sciences, precision and accuracy testing is achieved by dispensing a tartrazine solution into microplates containing water. This is referred to as a "liquid-to-liquid" dispense. The purpose of this paper is to report the performance obtained by dispensing 0.5 to 2μL aqueous samples containing tartrazine into 96 well plates containing water. Precision and accuracy results are shown for this work.

Materials and Methods

Instrumentation
Testing was performed using a RS-232 RapidPlate 96/384 Workstation (Caliper Life Sciences) using 100μL Automation Certified Pipette TipsTM (Caliper Life Sciences). A Bulk Dispenser (Caliper Life Sciences) was utilized for bulk dispensing across the columns of the microplate (secondary dispensing). Absorbance was measured at 450nm using a Wallac Victor2 Multilabel Counter (PerkinElmer).

Reagents
Tartrazine (Sigma Chemical Company, Catalogue number T-0388) is dissolved in deionized water. Concentrations of 6.0, 1.4, 0.7 and 0.14g/L corresponding to both 1 and 2, 5, 10 and 25μL dispenses respectively are prepared by dissolving the tar
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
3. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
4. Sciclone ALH Bulk Dispense Precision Testing
5. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
6. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
7. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:7/29/2015)... BIRMINGHAM, Ala., July 29, 2015 /PRNewswire/ ... the nation,s largest providers of post-acute healthcare services, ... reported its results of operations for the second ... second quarter was characterized by strong volume growth ... our Adjusted EBITDA," said Jay Grinney, President and ...
(Date:7/29/2015)... MILWAUKEE, Wis. (PRWEB) , ... July 29, 2015 ... ... facility identification solutions, today announced its GHS Label Guide . To align ... Harmonized System (GHS), this guide includes explanations of label components, an example of ...
(Date:7/29/2015)... , July 29, 2015  Indivior PLC (LON: INDV) ... naloxone nasal spray was accepted and received Priority Review ... the treatment of opioid overdose. This naloxone nasal spray ... formulated for optimal absorption into the nasal mucosa. 1 ... training so individuals may be better equipped to help ...
(Date:7/29/2015)... Francisco, CA (PRWEB) , ... July 29, 2015 , ... ... in the morning is quite normal. Although there are a considerable number of terms ... well understood that this post-sleep material is a mixture of mucus, blood cells, skin ...
Breaking Biology Technology:HealthSouth Reports Results for Second Quarter 2015 2HealthSouth Reports Results for Second Quarter 2015 3HealthSouth Reports Results for Second Quarter 2015 4HealthSouth Reports Results for Second Quarter 2015 5HealthSouth Reports Results for Second Quarter 2015 6HealthSouth Reports Results for Second Quarter 2015 7HealthSouth Reports Results for Second Quarter 2015 8HealthSouth Reports Results for Second Quarter 2015 9HealthSouth Reports Results for Second Quarter 2015 10HealthSouth Reports Results for Second Quarter 2015 11HealthSouth Reports Results for Second Quarter 2015 12HealthSouth Reports Results for Second Quarter 2015 13HealthSouth Reports Results for Second Quarter 2015 14HealthSouth Reports Results for Second Quarter 2015 15HealthSouth Reports Results for Second Quarter 2015 16HealthSouth Reports Results for Second Quarter 2015 17HealthSouth Reports Results for Second Quarter 2015 18HealthSouth Reports Results for Second Quarter 2015 19HealthSouth Reports Results for Second Quarter 2015 20HealthSouth Reports Results for Second Quarter 2015 21HealthSouth Reports Results for Second Quarter 2015 22HealthSouth Reports Results for Second Quarter 2015 23HealthSouth Reports Results for Second Quarter 2015 24HealthSouth Reports Results for Second Quarter 2015 25HealthSouth Reports Results for Second Quarter 2015 26HealthSouth Reports Results for Second Quarter 2015 27HealthSouth Reports Results for Second Quarter 2015 28HealthSouth Reports Results for Second Quarter 2015 29HealthSouth Reports Results for Second Quarter 2015 30HealthSouth Reports Results for Second Quarter 2015 31HealthSouth Reports Results for Second Quarter 2015 32HealthSouth Reports Results for Second Quarter 2015 33HealthSouth Reports Results for Second Quarter 2015 34HealthSouth Reports Results for Second Quarter 2015 35HealthSouth Reports Results for Second Quarter 2015 36
... , WALTHAM, Mass., Oct. 20 Inverness Medical Innovations, Inc. ... to take charge of their health at home through the ... it will release its third quarter 2009 earnings on Tuesday, ... call beginning at 10:00 a.m. (Eastern Time) on that date ...
... Transdel Pharmaceuticals, Inc. (OTC Bulletin Board: TDLP), a specialty ... its proprietary transdermal delivery platform, today announced that Dr. ... will present a company overview at two upcoming ... annual BIOCOM Investor Conference in San Diego, CA on ...
... 20 GeoVax Labs, Inc. (OTC Bulletin ... Atlanta-based, biopharmaceutical company developing human vaccines for diseases caused ... today announced that it will move its corporate headquarters ... University to Smyrna, Georgia, a northwest suburb of Atlanta, ...
Cached Biology Technology:Inverness Medical Innovations Schedules Conference Call for 10:00 a.m. ET October 27, 2009 to Discuss Third Quarter 2009 Results 2Transdel Pharmaceuticals to Present at Two Investor Conferences 2Transdel Pharmaceuticals to Present at Two Investor Conferences 3GeoVax Labs, Inc. Moving Operations to Smyrna, Georgia 2GeoVax Labs, Inc. Moving Operations to Smyrna, Georgia 3GeoVax Labs, Inc. Moving Operations to Smyrna, Georgia 4
(Date:7/27/2015)... , July 27, 2015  Synaptics Inc. (NASDAQ: ... interface solutions, today announced that Huawei has selected ... solution for its stylish smartwatch. Huawei chose the ... reliability, low power and highly responsive human interface ... Huawei designers also required a classic round watch ...
(Date:7/21/2015)... July 21, 2015 Today, ZTE announced its ... as well as expected revenues in 2015 that relate to sales ... revenue guidance of approximately 2,200 MSEK for 2015. Jörgen ... leading smartphone manufacturer in China and we ... 02 5 for Axon , ...
(Date:7/13/2015)... , July 13, 2015 Synaptics Inc. ... human interface solutions, today announced sampling of ClearPad ... display driver integration (TDDI) product targeting smartphones and ... to combine Synaptics , best-in-class touch controller ... driver technology developed in the company,s Japan Design ...
Breaking Biology News(10 mins):Synaptics Touchscreen Solution Powers New Huawei Watch 2Synaptics Touchscreen Solution Powers New Huawei Watch 3FPC's Touch Fingerprint Sensor FPC1025 in ZTE's Smartphone Axon 2Synaptics Announces Sampling of Second Generation TDDI Solutions 2Synaptics Announces Sampling of Second Generation TDDI Solutions 3
... If you carried a gene that increased your risk ... know? Do perceptions of stigma and discrimination against people ... disease? Sara Shostak, assistant professor in the department ... of epidemiology at Columbia University, and other collaborators were ...
... - Mercyhurst University biologists have received a $30,000 grant to ... woolly adelgid, a small, aphidlike insect that is decimating the ... considered one of the greatest threats to our eastern forests; ... Georgia," said Michael Elnitsky, assistant professor of biology, who wrote ...
... Institute at Oregon State University have discovered yet another reason ... is so good for you it provides not just ... mechanism of epigenetics. Epigenetics, an increasing focus of research ... but also to the way that diet, toxins and other ...
Cached Biology News:Grant to fund research into psychosocial impacts of epilepsy testing 2Grant to fund research into psychosocial impacts of epilepsy testing 3Mercyhurst research aims to predict impact of invasive forest pest 2Another mechanism discovered by which sulforaphane prevents cancer 2