( is study we demonstrate the high level...)96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array

is study, we demonstrate the high level of precision and accuracy that can be expected of the Sciclone ALH Bulk Reagent Dispenser and the Low Volume Head with the 384 Fixed-Cannula Array. We achieved accuracy levels of 0.03 % for a volume of 50μL of water with the Bulk Dispenser. The dispense precision for the fixed cannulas were 0.52%, 0.22% and 3.07% for volumes of 50μL, 20μL and 1μL respectively. And accuracy levels of less than 1% for volumes of 50μL and 20μL with the low volume head and a 384 fixed cannula array.

The experimental data shows that excellent pipetting performance can be obtained from the Sciclone ALH (low volume head) equipped with the 384 Fixed-Cannula Array. The results surpass the published factory specifications ( CV <= 8%) at 1μL dispense volumes, likely as a result of good pipetting technique. We believe this % CV can even be reduced by using degassed water. We hadn't the opportunity to do so at the test facility.

We have found that residual on the outside of the cannulas becomes the most influential liquid when determining CV's in low volume dispensing. Optimized touch offs in combination with time delays at tip touches and very slow draw backs out of liquids increases remarkably the performance of the pipettor.

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