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96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array

he plates before or after (with chase) the tartrazine dispense. For our work, the 384 fixed-cannula array on the ALH (Caliper Life Sciences) was used.

Qualification Results
A gravimetric dispense test is done to determine the accuracy of the bulk (Table 1) and 384 fixed -cannula array (Table 2) dispense operations.

Fixed Cannula Precision Determination (1μL dispensing)

a. Water (20μL) was dispensed into each column of a 384-well plate with the Bulk Dispenser in a separate routine. Starting with a 3μL air gap, Tartrazine (5μL) was aspirated in a larger volume than needed (10μL). While still in the reservoir a 5μL volume was pushed out once. This leaves 5μL in the cannulas and, more importantly, residual on the outside of the cannulas. To control this the cannulas were touched off using a low volume dispense (0.2μL) on the surface of an upside down flat bottom plate followed by a small vertical tip touch. Finally the Tartrazine was consecutive dispensed (1μL) into each quadrant of a 384 well plate with the low volume head.

b. Starting with a 3μL air gap, a chase water volume of 20μL was aspirated followed by a 1μL air gap. Then tartrazine (1μL) was aspirated and finally the whole content of the cannulas was dispensed into a quadrant of a 384 well plate with the low volume head.

Precision Results
Absorbance was measured and the data exported to ExcelTM for CV determination. The results of the dispensed data for fixed cannula dispensing are shown below. The overall CV's that compromise every well of a 384 well plate are shown for each procedure tested. The average %CV per procedure is calculated in each table.

Discussion

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