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96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array

Rudy Willebrords1 and Bart Moyson2
1Johnson & Johnson Pharmaceutical Research & Development, a division of Janssen Pharmaceutica N.V., Laboratory of Virology, Beerse, Belgium
2Caliper Life Sciences Benelux NV, Teralfene, Belgium

Introduction

The purpose of this paper is to report the performance obtained by dispensing 1μL organic sample (DMSO) containing tartrazine into 384 well plates containing water. Precision and accuracy results are shown for this work.

Instrumentation
All testing was performed using a Sciclone Advanced Liquid Handler (ALH) with a 384 Fixed-Cannula Array (Caliper Life Sciences). A Bulk Reagent Dispenser (Caliper Life Sciences) facilitated the continuous delivery of water across the columns of a 384 Well, Flat Bottom, Untreated, Polystyrene Assay plates (COSTAR Catalogue # 9837). Absorbance was measured at two wavelengths using a SPECTRAmax (Molecular Devices Corporation) 2000/384 reader (690 nm is subtracted from the 450 nm).

Reagents
Tartrazine (Sigma) solution are prepared in dimethyl sulfoxide (DMSO) at 3.0 g/L and dispensed at 1μL.

Qualification of the Bulk Reagent Dispenser
Qualification of the bulk reagent dispenser is done to confirm the accuracy of the volume dispensed. This bulk dispenser is qualified by dispensing volumes of water into a plate, which is then weighed on a balance, and the accuracy calculated. For our work, the Bulk Reagent Dispenser on the ALH (Caliper Life Sciences) was used.

Qualification of the Fixed-Cannula Array
Qualification of the 384 fixed array is done to confirm the accuracy of the volume dispensed. When testing the ALH for 1μL volume dispensing, 20μL water is added to t
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