Navigation Links
721.82

Multiporator Transfection Protocol Protocol No. 4308 915.045 11/2001 Cell line 721.82, EBV transfected B cell line, MHC class I positive,
MHC class II DR, DQ negative Transfection with Plasmid pEGFP-N1 (in bidistilled H2O) Electroporation buffer Eppendorf Hypoosmolar Electroporation Buffer (PH) Culture medium RPMI / 10% FCS Cuvette Eppendorf, 2 mm gap width, 400 l Temperature RT (20-25 C) Reference Dr. Kirsten Falk and Dr. Olaf Rtzschke Cellular Immunology
Max Delbrck Center for Molecular Medicine Robert-Rssle-Strasse 10 D-13125 Berlin-Buch
  1. Harvest the cells in the exponential growth phase and centrifuge them (for 5 minutes, 200 x g, at room temperature).
  2. Resuspend the cells in RPMI / 0.5% FCS, determine the number of cells and centrifuge them (for 5 minutes, 200 x g, at room temperature). Remove supernatant.
  3. Resuspend the cells in medium, set the cell number to 1 x 106 cells/ml and centrifuge them (for 5 minutes, 200 x g, at room temperature). Remove supernatant.

    Note: The overall incubation time in the Eppendorf Electroporation Buffer must not exceed 30 min
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. 721.221
Post Your Comments:
*Name:
*Comment:
*Email:
TAG:

(Date:1/15/2014)... January 15, 2014 This webinar ... on nonclinical and clinical safety assessment in biosimilars. ... guide a path for biosimilar drug development, however ... of biosimilarity with regards to quality, safety and ... of biosimilar drug development and registration, development strategies ...
(Date:1/15/2014)... NC (PRWEB) January 15, 2014 ... an enhancement to its Online Web Portal for Life ... now have the ability to specify the subject matter ... nearly 50 life science sub-domains. This will help reduce ... and scientific fields, and decrease the likelihood of error. ...
(Date:1/14/2014)... Doylestown, PA (PRWEB) January 14, 2014 ... – 11 p.m. , Location: Warrington Country Club, 1360 ... Hepatitis B Foundation, the only national nonprofit organization solely ... improving the quality of life for those affected worldwide, ... 11 at Warrington Country Club in Warrington, Pa. ...
(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... initiated coverage of Alliqua, Inc. (OTCQB: ALQA). Alliqua ... and marketing proprietary products to serve the wound ... , In late 2012, Alliqua was restructured ... launched the company’s new strategy to become a ...
Breaking Biology Technology:Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... AMSTERDAM, February 20 Elsevier announced today,that ... abstract and,citation (A&C) database, was chosen by ... in Research for Australia (ERA) initiative. , ... will assess research quality within Australia,s higher,education ...
... of the world,s most versatile and widely used chemicals. ... is commonly used as a bleach, an antiseptic and ... J. Kiely, hydrogen peroxide has eluded the best efforts ... environmentally friendly means of producing it. , "Hydrogen peroxide ...
... Safety Study in Patients with Severe Post-Operative Pain; ... and Support NDA SubmissionSYDNEY and BEDMINSTER, N.J., Feb. ... QRXPY), a clinical-stage specialty pharmaceutical company focused on ... and central nervous system (CNS) disorders, announced today ...
Cached Biology Technology:The Australian Research Council Selects Scopus for the Evaluation of the Australian Research Landscape 2The Australian Research Council Selects Scopus for the Evaluation of the Australian Research Landscape 3Gold-palladium nanoparticles achieve greener, smarter production of hydrogen peroxide 2Gold-palladium nanoparticles achieve greener, smarter production of hydrogen peroxide 3Gold-palladium nanoparticles achieve greener, smarter production of hydrogen peroxide 4QRxPharma Initiates Second Comparative Study for MoxDuo(TM) IR Dual-Opioid(TM) Pain Therapy 2QRxPharma Initiates Second Comparative Study for MoxDuo(TM) IR Dual-Opioid(TM) Pain Therapy 3QRxPharma Initiates Second Comparative Study for MoxDuo(TM) IR Dual-Opioid(TM) Pain Therapy 4
(Date:4/15/2014)... 2014, Irvine, Calif. UC Irvine neurobiologists have found ... when transplanted into the brains of mice with the ... is published in the journal Stem Cells Research ... to work in two different mouse models. , ... dementia, is associated with accumulation of the protein amyloid-beta ...
(Date:4/15/2014)... know as early as 2020 how high sea levels ... team of researchers including Florida International University scientist Rene ... Science Foundation,s (NSF) Florida Coastal Everglades Long-Term Ecological Research ... in ecosystems from coral reefs to deserts, mountains to ... sea level rise is one of the most certain ...
(Date:4/15/2014)... UC San Diego have succeeded in visualizing the ... for growth and resistance to drought. The achievement ... determine how the hormone helps plants respond to ... continuing increase in the atmosphere,s carbon dioxide, or ... in the April 15 issue of the scientific ...
Breaking Biology News(10 mins):UCI study finds modified stem cells offer potential pathway to treat Alzheimer's disease 2Long-term predictions for Miami sea level rise could be available relatively soon 2Biologists develop nanosensors to visualize movements and distribution of plant hormone 2
... earths system of mid-ocean ridges plays a pivotal role in ... heat flow from the interior, and the chemistry of rock, ... that scientists discovered the existence of vast plumbing systems under ... spit it back out from seafloor ventsa process that brings ...
... of the most common human parasites, Toxoplasma gondii, uses a ... increase its numbers and when to remain dormant, researchers at ... The scientists report this week in Nature that they ... (ABA), with a plant herbicide. Low doses of the herbicide ...
... Association of Water Companies (NAWC) held its annual ... Bob Iacullo presented the 2007 Management Innovation Awards. ... to share innovative approaches to issues relevant to ... to United Water for the entry Senior Design ...
Cached Biology News:Quakes under Pacific floor reveal unexpected circulatory system 2Quakes under Pacific floor reveal unexpected circulatory system 3Disrupting common parasites' ability to 'talk' to each other reduces infection 2Disrupting common parasites' ability to 'talk' to each other reduces infection 3United Water, in partnership with Stevens, wins Management Innovation Award 2
... (eIF4E-binding protein II) is involved in translation ... kidney. PHAS proteins bind to eIF-4E, the ... capped mRNA in vitro and in cells. ... of PHAS/eIF-4E complexes. This allows eIF-4E to ...
... 2 (VMAT2). An antibody made to the ... at ~55 kDa in postnuclear supernatants of ... wild type cells (2). Some additional bands, ... also detected and remain unaffected by the ...
... Anti-Matrix Metalloproteinase-2, Hinge Region By immunoblotting ... native protein. It reacts with free MMP-2 ... antibody buffered aqueous glycerol solution ... pH 7.4, containing 50% glycerol and 15 ...
Acetylcholinesterase from human and monkey....
Biology Products: