Navigation Links
7 Programming

77.6.6 Sample cooling and subsequent PCR

If sensitive samples should remain cooled until the PCR takes place, the thermoblock must be preheated. This
can be carried out in two ways:

  1. Set the "Incu" menu to 4 C and "Heater is On".
  2. Adapt an initial program and connect via a "LINK" command.
    For this purpose, the temperature of the thermoblock is brought to 4 C using the first program. After the
    samples have been inserted into the cold thermoblock, the second program with the PCR protocol is
    called up via a LINK command.

Programming should take place as follows:

Program 1 e.g. COOL CNTRL TUBE
Lid
NOWAIT FIX
The entries in the first program are also valid for the programs which are added via LINK. The lid setting "Fix" must be selected. 1 Hold 4 C Enter

2. Link PCRPROT
end
The thermoblock cools until the processing program (e.g. PCRPROT) is started
via the LINK command by pressing .
Link to the second program with the PCR protocol. Program 2 PCRPROT CNTRL TUBE If the program also contains the "Tube control" command, further entries for
tube type and filling volume are no longer necessary; the entries made for the
first program are valid for all further programs.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. 7 Programming
2. 7 Programming
3. 7 Programming
4. 7 Programming
5. 7 Programming
6. Mastercycler Gradient Quick Programming Guide
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)...  Blueprint Bio, a company dedicated to identifying, protecting ... has closed its Series A funding round, according to ... "We have received a commitment from Forentis Fund that ... meet our current goals," stated Matthew Nunez . ... complete validation on the current projects in our pipeline, ...
(Date:6/23/2016)... , June 23, 2016 On ... session at 4,833.32, down 0.22%; the Dow Jones Industrial Average ... 500 closed at 2,085.45, down 0.17%. Stock-Callers.com has initiated coverage ... INFI ), Nektar Therapeutics (NASDAQ: NKTR ), Aralez ... Inc. (NASDAQ: BIND ). Learn more about these ...
(Date:6/23/2016)... ... 23, 2016 , ... Regulatory Compliance Associates® Inc. (RCA), a ... webinar on Performing Quality Investigations: Getting to Root Cause. This ... charge. , Incomplete investigations are still a major concern to the Regulatory Authorities ...
(Date:6/23/2016)... FRANCISCO , June 22, 2016  Amgen (NASDAQ: ... sponsorship of the QB3@953 life sciences incubator ... human health. The shared laboratory space at QB3@953 was ... overcome a key obstacle for many early stage organizations ... part of the sponsorship, Amgen launched two "Amgen Golden ...
Breaking Biology Technology:
(Date:4/14/2016)... 2016 BioCatch ™, the ... announced the appointment of Eyal Goldwerger as ... Goldwerger,s leadership appointment comes at a time of ... deployment of its platform at several of the world,s ... discerns unique cognitive and physiological factors, is a winner ...
(Date:3/31/2016)... March 31, 2016   ... or the "Company") LegacyXChange is excited to ... its soon to be launched online site for trading ... ) will also provide potential shareholders a sense ... technology to an industry that is notorious for fraud. ...
(Date:3/29/2016)... Florida , March 29, 2016 ... the "Company") LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased ... in ink used in a variety of writing instruments, ... Buyers of originally created collectibles from athletes on LegacyXChange ... forensic analysis of the DNA. Bill ...
Breaking Biology News(10 mins):