Navigation Links
7 Programming

77.6.6 Sample cooling and subsequent PCR

If sensitive samples should remain cooled until the PCR takes place, the thermoblock must be preheated. This
can be carried out in two ways:

  1. Set the "Incu" menu to 4 C and "Heater is On".
  2. Adapt an initial program and connect via a "LINK" command.
    For this purpose, the temperature of the thermoblock is brought to 4 C using the first program. After the
    samples have been inserted into the cold thermoblock, the second program with the PCR protocol is
    called up via a LINK command.

Programming should take place as follows:

Program 1 e.g. COOL CNTRL TUBE
The entries in the first program are also valid for the programs which are added via LINK. The lid setting "Fix" must be selected. 1 Hold 4 C Enter

The thermoblock cools until the processing program (e.g. PCRPROT) is started
via the LINK command by pressing .
Link to the second program with the PCR protocol. Program 2 PCRPROT CNTRL TUBE If the program also contains the "Tube control" command, further entries for
tube type and filling volume are no longer necessary; the entries made for the
first program are valid for all further programs.



Page: All 1 2

Related biology technology :

1. 7 Programming
2. 7 Programming
3. 7 Programming
4. 7 Programming
5. 7 Programming
6. Mastercycler Gradient Quick Programming Guide
Post Your Comments:

(Date:6/27/2016)... CLEVELAND , June 27, 2016  Global ... average 4.6 percent through 2020 to $7.2 billion.  ... (food and beverages, cleaning products, biofuel production, animal ... and biotechnology, diagnostics, and biocatalysts). Food and beverages ... gains driven by increasing consumption of products containing ...
(Date:6/27/2016)... ... June 27, 2016 , ... ... innovative medical technologies, services and solutions to the healthcare market. The company's primary ... various distribution, manufacturing, sales and marketing strategies that are necessary to help companies ...
(Date:6/24/2016)... Epic Sciences unveiled a liquid biopsy ... PARP inhibitors by targeting homologous recombination deficiency (HRD) ... test has already been incorporated into numerous clinical ... Over 230 clinical trials are investigating ... PARP, ATM, ATR, DNA-PK and WEE-1. Drugs targeting ...
(Date:6/23/2016)... , June 23, 2016   Boston ... of novel compounds designed to target cancer stemness ... has been granted Orphan Drug Designation from the ... treatment of gastric cancer, including gastroesophageal junction (GEJ) ... inhibitor designed to inhibit cancer stemness pathways by ...
Breaking Biology Technology:
(Date:6/20/2016)... , June 20, 2016 Securus Technologies, ... technology solutions for public safety, investigation, corrections and ... prisons involved, it has secured the final acceptance ... facilities for Managed Access Systems (MAS) installed. Furthermore, ... facilities to be installed by October, 2016. MAS ...
(Date:6/9/2016)... June 9, 2016 Paris ... Teleste,s video security solution to ensure the safety of people ... during the major tournament Teleste, an international ... and services, announced today that its video security solution will ... to back up public safety across the country. The system ...
(Date:6/2/2016)... 2, 2016   The Weather Company , an IBM ... an industry-first capability in which consumers will be able to ... ask questions via voice or text and receive relevant information ... Marketers have long sought an advertising solution that ... be personal, relevant and valuable; and can scale across millions ...
Breaking Biology News(10 mins):