Navigation Links
7 Programming

77.6.6 Sample cooling and subsequent PCR

If sensitive samples should remain cooled until the PCR takes place, the thermoblock must be preheated. This
can be carried out in two ways:

  1. Set the "Incu" menu to 4 C and "Heater is On".
  2. Adapt an initial program and connect via a "LINK" command.
    For this purpose, the temperature of the thermoblock is brought to 4 C using the first program. After the
    samples have been inserted into the cold thermoblock, the second program with the PCR protocol is
    called up via a LINK command.

Programming should take place as follows:

Program 1 e.g. COOL CNTRL TUBE
Lid
NOWAIT FIX
The entries in the first program are also valid for the programs which are added via LINK. The lid setting "Fix" must be selected. 1 Hold 4 C Enter

2. Link PCRPROT
end
The thermoblock cools until the processing program (e.g. PCRPROT) is started
via the LINK command by pressing .
Link to the second program with the PCR protocol. Program 2 PCRPROT CNTRL TUBE If the program also contains the "Tube control" command, further entries for
tube type and filling volume are no longer necessary; the entries made for the
first program are valid for all further programs.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. 7 Programming
2. 7 Programming
3. 7 Programming
4. 7 Programming
5. 7 Programming
6. Mastercycler Gradient Quick Programming Guide
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/3/2016)... -- With the growing need for better therapeutics, and ... such as monoclonal antibodies, recombinant protein therapeutics and ... are in high demand. Conventionally expression systems were ... of these therapeutics. However, due to issues with ... approaches and novel expression systems are currently being ...
(Date:2/3/2016)... , ... February 03, 2016 , ... ... established a new office dedicated to the North American healthcare market. , Aerocom ... to healthcare facilities. The company will provide new pneumatic tube systems or ...
(Date:2/3/2016)... -- Silk Therapeutics, Inc., today announced the closing of a $6 ... a total of $10.25 million in Series A funding based ... round was led by existing investor The Kraft Group of ... investors Lear Corporation and Highland Consumer Partners, as well as ... Richard Sackler , MD, with Summer Road, LLC; Erin ...
(Date:2/3/2016)... YORK. (PRWEB) , ... February 03, 2016 , ... ... of silicon (Si) and InGaAs chips and wafers, and InP epi wafers based ... ranging from silicon detectors–including photodiodes, photo transistors, and Avalanche photodiodes–to Si and InGaAs ...
Breaking Biology Technology:
(Date:2/2/2016)... Feb. 2, 2016 This BCC Research ... market by reviewing the recent advances in high ... drive the field forward. Includes forecast through 2019. ... the challenges and opportunities that exist in the ... solution developers, as well as IT and bioinformatics ...
(Date:2/1/2016)... February 1, 2016 Rising sales ... drive global touchfree intuitive gesture control market ... Rising sales of consumer electronics coupled with new technological ... size through 2020   ... with new technological advancements to drive global touchfree intuitive ...
(Date:1/25/2016)... Jan. 25, 2016   Unisys Corporation (NYSE: UIS ... F. Kennedy (JFK) International Airport, New York City ... identify imposters attempting to enter the United States ... them. pilot testing of the system at Dulles ... terminals at JFK during January 2016. --> pilot testing ...
Breaking Biology News(10 mins):