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7 Programming

77.6.6 Sample cooling and subsequent PCR

If sensitive samples should remain cooled until the PCR takes place, the thermoblock must be preheated. This
can be carried out in two ways:

  1. Set the "Incu" menu to 4 C and "Heater is On".
  2. Adapt an initial program and connect via a "LINK" command.
    For this purpose, the temperature of the thermoblock is brought to 4 C using the first program. After the
    samples have been inserted into the cold thermoblock, the second program with the PCR protocol is
    called up via a LINK command.

Programming should take place as follows:

Program 1 e.g. COOL CNTRL TUBE
Lid
NOWAIT FIX
The entries in the first program are also valid for the programs which are added via LINK. The lid setting "Fix" must be selected. 1 Hold 4 C Enter

2. Link PCRPROT
end
The thermoblock cools until the processing program (e.g. PCRPROT) is started
via the LINK command by pressing .
Link to the second program with the PCR protocol. Program 2 PCRPROT CNTRL TUBE If the program also contains the "Tube control" command, further entries for
tube type and filling volume are no longer necessary; the entries made for the
first program are valid for all further programs.


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