77.6.6 Sample cooling and subsequent PCR
If sensitive samples should remain cooled until the PCR takes place, the
thermoblock must be preheated. This
can be carried out in two ways:
- Set the "Incu" menu to 4 C and "Heater is On".
- Adapt an initial program and connect via a "LINK" command.
For this purpose, the temperature of the thermoblock is brought to 4
C using the first program. After the
samples have been inserted into the cold thermoblock, the second program
with the PCR protocol is
called up via a LINK command.
Programming should take place as follows:
The entries in the first program are also
valid for the programs which are added via LINK. The lid setting "Fix"
must be selected.
1 Hold 4 C Enter
2. Link PCRPROT
The thermoblock cools until the processing program (e.g.
PCRPROT) is started
via the LINK command by pressing .
Link to the second program with the PCR protocol.
If the program also contains the "Tube control"
command, further entries for
tube type and filling volume are no longer necessary; the entries
made for the
first program are valid for all further programs.
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