Navigation Links
7 Programming

7.6.5 PCR "Fingerprinting" (RAPD-PCR)

Variable heating and cooling rates, which can be programmed as desired with each temperature command,
enable the temperature-control speed to be aligned to the temperature.

With the RAPD-PCR process or with PCR with A/T-rich primer/template hybrids, it may be necessary to heat
up carefully after annealing.

In the example given, the elongation temperature is attained at a speed of 1 C/s.
Temperature-control speeds, which may be as slow as the user desires, can be selected by means of program
loops, with which a gradual change in temperature is obtained via temperature increments.

In the following example, the elongation temperature is raised slowly to 72 C after annealing with the aid of a
program loop.



Page: All 1

Related biology technology :

1. 7 Programming
2. 7 Programming
3. 7 Programming
4. 7 Programming
5. 7 Programming
6. Mastercycler Gradient Quick Programming Guide
Post Your Comments:

(Date:11/25/2015)... , Nov. 25, 2015 /PRNewswire/ - Aeterna Zentaris Inc. ... its business and prospects remain fundamentally strong and ... (zoptarelin doxorubicin) recently received DSMB recommendation to continue ... following review of the final interim efficacy and ... Primary Endpoint in men with heavily pretreated castration- ...
(Date:11/24/2015)... Malaysia , Nov. 24, 2015  Asia-Pacific ... contract research organisation (CRO) market. The trend of ... in lower margins but higher volume share for ... capacity and scale, however, margins in the CRO ... Organisation (CRO) Market ( ), finds ...
(Date:11/24/2015)... ... 2015 , ... Copper is an essential micronutrient that all ... copper is also toxic to cells. With a $1.3 million award from the ... a systematic study of copper in the bacteria Pseudomonas aeruginosa (P. aeruginosa), a ...
(Date:11/24/2015)... , ... November 24, 2015 , ... This fall, global ... competitive events in five states to develop and pitch their BIG ideas to improve ... each state are competing for votes to win the title of SAP's Teen Innovator, ...
Breaking Biology Technology:
(Date:11/18/2015)... November 18, 2015 --> ... a new market report titled  Gesture Recognition Market - ... 2015 - 2021. According to the report, the global gesture recognition ... anticipated to reach US$29.1 bn by 2021, at a ... North America dominated the global gesture ...
(Date:11/17/2015)... 17, 2015 Pressure BioSciences, Inc. (OTCQB: PBIO) ... and sale of broadly enabling, pressure cycling technology ("PCT")-based ... today announced it has received gross proceeds of $745,000 ... Placement (the "Offering"), increasing the total amount raised to ... additional closings are expected in the near future. ...
(Date:11/12/2015)... --  Growing need for low-cost, easy to use, ... the way for use of biochemical sensors for ... clinical, agricultural, environmental, food and defense applications. Presently, ... applications, however, their adoption is increasing in agricultural, ... on improving product quality and growing need to ...
Breaking Biology News(10 mins):