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7 Programming

7.6.5 PCR "Fingerprinting" (RAPD-PCR)

Variable heating and cooling rates, which can be programmed as desired with each temperature command,
enable the temperature-control speed to be aligned to the temperature.

With the RAPD-PCR process or with PCR with A/T-rich primer/template hybrids, it may be necessary to heat
up carefully after annealing.

In the example given, the elongation temperature is attained at a speed of 1 C/s.
Temperature-control speeds, which may be as slow as the user desires, can be selected by means of program
loops, with which a gradual change in temperature is obtained via temperature increments.

In the following example, the elongation temperature is raised slowly to 72 C after annealing with the aid of a
program loop.



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