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7 Programming

7.6.2 Hot Start PCR

The "Pause" command is used to interrupt the program. The most-recently entered temperature is
maintained. A text explaining the reason for the interruption may be entered as required. After confirmation by
pressing , the program is continued.

The "Hold" command enables all possible temperatures to be maintained until the program is continued by
pressing .

In the exampleof a Hot Start PCR listed below, the program is stopped folllowing initial denaturation in order to
add a reaction component. After the final elongation, the block is cooled down to room temperature.



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